Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-30T20:33:29.349Z Has data issue: false hasContentIssue false
  • English
  • Français

IN VITRO AND ECUADOR-FIELD PERFORMANCE OF VIRUS-TESTED AND VIRUS-INFECTED PLANTS OF TROPAEOLUM TUBEROSUM

Published online by Cambridge University Press:  10 July 2009

S. SORIA RE ,
C. TABOADA ,
R. VEGA GONZALEZ ,
T. EVANS ,
V. D. DAMSTEEGT  and
S. KITTO
S. SORIA RE
Affiliation:
Department of Plant and Soil Sciences, University of Delaware, Newark, DE 19716-2170, USA
C. TABOADA
Affiliation:
AMDE Corp., Las Tunas 104, Ambato, Ecuador
R. VEGA GONZALEZ
Affiliation:
AMDE Corp., Las Tunas 104, Ambato, Ecuador
T. EVANS
Affiliation:
Department of Plant and Soil Sciences, University of Delaware, Newark, DE 19716-2170, USA
V. D. DAMSTEEGT
Affiliation:
Foreign Disease-Weed Science Research Unit, Building 1301, Fort Detrick, Frederick, MD 21702, USA
S. KITTO*
Affiliation:
Department of Plant and Soil Sciences, University of Delaware, Newark, DE 19716-2170, USA
*
‡‡Corresponding author: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Ecuadorean mashua (Tropaeolum tuberosum) germplasm has been found to be widely infected with the potyvirus Tropaeolum mosaic virus (TropMV). The objective of this research was to produce virus-tested (VT) germplasm to compare growth in vitro and in the field with virus-infected (V) germplasm. Twenty-three of 25 apical dome-derived clones tested free of virus based on bioassays using Nicotiana benthamiana and Chenopodium quinoa. In vitro-generated plant tissue was just as effective for determining VT status as greenhouse-generated plant tissue. Genotype rather than virus-infection status appeared to have a greater effect on in vitro proliferation. There were no differences in in vitro rooting among the genotypes or between the VT clones compared to the V clones, with at least 90% of the microcuttings rooting. However, rooted microcuttings of V clones were taller than rooted microcuttings of VT clones. Plants were readily re-established in a greenhouse at the USDA, Foreign Disease-Weed Science Research Unit at Fort Detrick, USA. In field experiment 1, ca. 75% of the plants survived field transplanting and VT plants of genotype 1147 had greater tuber weight (928 g) than V plants (235 g). In field experiment 2, plant mortality was high one month after field transplanting. Genotypes 1093 (59%) and 1141 (54%) had higher survival than genotype 1147 (44%); however, survival did not differ between the VT (46%) and V (59%) plants of all genotypes. No differences were noted in field performance for the three genotypes after 10 months of growth. Although overall tuber yield among the V, VT and VTR (reinfected-VT plants) did not differ, V plants produced big tubers that weighed more than those from VT plants. Thirty-three percent of the VT plants became reinfected and 42% of the V plants tested negative after 10 months in the field based on double-antibody-sandwich ELISA.

Type
Research Article
Information
Experimental Agriculture , Volume 45 , Issue 4 , October 2009 , pp. 483 - 497
Copyright
Copyright © Cambridge University Press 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Alfredo, G., Dueñas, R. O., Cabrera, C.N. and Hermann, M. (2003). Mashua (Tropaeolum tuberosum Ruíz and Pav.). Promoting the Conservation and Use of Underutilized and Neglected Crops. 25. International Potato Center, Lima, Peru/International Plant Genetic Resources Institute, Rome, Italy.Google Scholar
Bartels, R. (1954). Serologische untersuchungen über das Verhalten des Kartoffel-A-virus in Tabakpflanzen. Phytopathologische Zeitschrift 21:395–40.Google Scholar
Bitterlin, M. W., Gonsalves, G. D. and Cummins, J. N. (1984). Irregular distribution of tomato ringspot virus in apple trees. Plant Disease 68:567–571.CrossRefGoogle Scholar
Brown, C. R., Kwiatkowski, S., Martin, M. W. and Thomas, P. E. (1988). Eradication of PVS from potato clones through excision of meristems from in vitro, heat-treated shoot tips. American Potato Journal 65:633–638.Google Scholar
Clark, C. A. and Hoy, M. W. (2006). Effects of common viruses on yield and quality of Beauregard sweetpotato in Louisiana. Plant Disease 90:83–88.CrossRefGoogle ScholarPubMed
De Vries-Paterson, R. M., Evans, T. A. and Stephens, C. T. (1992). The effect of asparagus virus infection on asparagus tissue culture. Plant Cell, Tissue and Organ Culture 31:31–35.Google Scholar
Delhey, R. and Monasterios, T. (1977). A mosaic disease of isañu (Tropaeolum tuberosum) from Bolivia. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz 84:224–231.Google Scholar
Estrada, R., Manya, W., Pulache, C., Sanchez, H. and Yonamine, T. (1986). Maintenance, micropropagation and seed production of the Andean tuber crops: oca, olluco and mashua. In Abstracts of the International Congress of Plant Tissue and Cell Culture. University of Minnesota, Minneapolis, USA. August 3–8, 1986, 103.Google Scholar
Fandiño, T. J., Torres, O. and Perea-Dallos, M. (1987). Morfogénesis y microporpagación de Tropaeolum tuberosum (Ruíz y Pavón). Boletín Científico de la Asociación Colombiana de Estudios Vegetales In Vitro (ACEVIV) 1:29–35.Google Scholar
Food and Agriculture Organization. (1990). Guía para el manejo de plagas en cultivos Andinos subexplotados. Santiago (Chile), Food and Agriculture Organization of the United Nations, p. 110.Google Scholar
Guimarães, R. L. and Flores, H. E. (2005). Tropaeolum mosaic potyvirus (TropMV) reduces yield of Andean mashua (Tropaeolum tuberosum) accessions. HortScience 40:1405–1407.Google Scholar
Gutiérrez, D. L., Fuentes, S. and Salazar, L. F. (2003). Sweetpotato virus disease (SPVD): Distribution, incidence, and effect on sweetpotato yield in Peru. Plant Disease 87:297–302.CrossRefGoogle ScholarPubMed
King, S. R. and Gershoff, S. N. (1987). Nutritional evaluation of three under exploited Andean tubers: Oxalis tuberosa (Oxalidaceae), Ullucus tuberosus (Basellaceae), and Tropaeolum tuberosum (Tropaeolaceae). Economic Botany 41:503–511.CrossRefGoogle Scholar
Lakshmanan, P., Geijskes, R. J., Aitken, K. S., Grof, C. L. P., Bonnett, G. D. and Smith, G. R. (2005). Invited Review: Sugarcane biotechnology: The challenges and opportunities. In Vitro Cellular & Developmental Biology – Plant 41:345–363.CrossRefGoogle Scholar
Matthews, R. E. F. (1992). Fundamentals of Plant Virology, 3rd edn. San Diego: Academic Press, Inc.Google Scholar
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum 15:473–497.Google Scholar
National Research Council. (1989). Lost Crops of the Incas: Little Known Plants of the Andes with Promise for Worldwide Cultivation. Washington, D. C.: National Academy Press.Google Scholar
Perea-Dallos, M., Fandiño, T. J. and Torres, O. (1986). Morphogenesis and micropropagation of Tropaeolum cell culture. Abstracts of the International Congress of Plant Tissue and Cell Culture. University of Minnesota, Minneapolis, USA. August 3–8, 1986, 104.Google Scholar
Robson, J. D., Wright, M. G. and Almeida, R. P. P. (2006). Within-plant distribution and binomial sampling of Pentalonia nigronervosa (Hemiptera: Aphididae) on banana. Journal of Economic Entomology 99:2185–2190.CrossRefGoogle ScholarPubMed
Sánchez-Navarro, J. A., Carmen Cañizares, M., Cano, E.A. and Pallás, V. (2007). Plant tissue distribution and chemical inactivation of six carnation viruses. Crop Protection 26:1049–1054.Google Scholar
Satterthwaite, F. C. (1946). An approximate distribution of estimates of variance components. Biometrics Bulletin 2:110–114.Google Scholar
Soria, S. (1996). Identification and elimination of viruses from mashua (Tropaeolum tuberosum, Ruiz and Pavon) and methods for its micropropagation. MSc Thesis, University of Delaware, Newark, USA.Google Scholar
Soria, S., Rojas, R., Damsteegt, V. D., McDaniel, L., Kitto, S. and Evans, T. A. (1998). Occurrence and partial characterization of a new mechanically transmissible virus in mashua from the Ecuadorean highlands. Plant Disease 82:69–73.Google Scholar
Tapia, M. E. (1990). Cultivos Andinos subexplotados y su aporte a la alimentación. Santiago, Food and Agriculture Organization of the United Nations. p. 205.Google Scholar
Walkey, D. G. A., Creed, C., Delaney, H. and Whitwell, J.D. (1981). Studies on the reinfection and yield of virus-tested and commercial stocks of rhubarb cv. Timperley Early. Plant Pathology 31:253–261.CrossRefGoogle Scholar
Wright, N. S. (1970). Combined effects of potato virus X and S on yield of Netted Gem and White Rose potatoes. American Potato Journal 47:475–478.Google Scholar
Wright, N. S. (1977). The effect of separate infections by potato viruses X and S on Netted Gem potato. American Potato Journal 54:147–149.Google Scholar
Xu, P., Chen, F., Mannas, J. P., Feldman, T., Sumner, L. W. and Roossinck, M. J. (2008). Virus infection improves drought tolerance. New Phytologist 180:911–921.Google Scholar
Zapata, C., Miller, J. C. Jr., and Smith, R. H. (1995). An in vitro procedure to eradicate potato viruses X, Y, and S from Russet Norkotah and two of its strains. In Vitro Cellular and Developmental Biology – Plant 31:153–159.Google Scholar