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Gene cloning

Published online by Cambridge University Press:  13 July 2009

Abstract

The experimental observations and techniques upon which the ‘Recombinant DNA’ revolution was founded are introduced; these often came from esoteric research, but nevertheless have promoted a dramatic change in both biotechnology and our molecular understanding of eukaryotic organisms. The basic approaches to the cloning and analysis of foreign genes in E. coli are described, followed by some indication of how the technologies continue to evolve, thereby enabling the current attack on major problems in agriculture and medicine.

Type
Research Article
Copyright
Copyright © Academia Europaea 1996

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References

REFERENCES

1.Murray, K. (1976) Biochemical manipulation of genes. Endeavour XXXV, 129133.CrossRefGoogle Scholar
2.Old, R. W. and Primrose, S. B. (1994) Principles of Gene Manipulation—An Introduction to Genetic, Engineering, 5th Ed, Blackwell Scientific, Oxford.Google Scholar
3.Kaiser, K., Murray, N. E. and Whittaker, P. A. (1995) DNA Cloning Vol. I, Edition II, edited by Glover, D. M. and Hames, B. D., IRL Press.Google Scholar
4.Hohn, B. and Collins, J. (1988) Ten years of cosmids. Tibtech 6, 293298.CrossRefGoogle Scholar
5.Nasmyth, K. and Sulston, J. (1987) High-altitude walking with YACs. Nature 328, 380381.CrossRefGoogle ScholarPubMed
6.Venter, J. C., Smith, H. O. and Hood, L. (1996) A new strategy for genome sequencing. Nature 381, 364365.CrossRefGoogle ScholarPubMed