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Serum of Bipolar Patients Induces Pro-inflammatory Activation of Macrophages

Published online by Cambridge University Press:  23 March 2020

S. Dubou Serafim ,
P. Ferrari ,
R. Colombo ,
L. Paul Géa ,
M. Migliorini Parisi ,
M. Becker ,
B.M. Ascoli ,
G. Fries ,
M. Kauer-Sant’anna  and
F. Kapczinski
...Show all authors
S. Dubou Serafim
Affiliation:
Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Psiquiatria e Ciências do Comportamento, Porto Alegre, Brazil
P. Ferrari
Affiliation:
Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Psiquiatria e Ciências do Comportamento, Porto Alegre, Brazil
R. Colombo
Affiliation:
Universidade de Caxias do Sul, Laboratory of Pharmacology and Physiology, Caxias do Sul, Brazil
L. Paul Géa
Affiliation:
Universidade Federal do Rio Grande do Sul, Department of Pharmacology and Postgraduate Program: Pharmacology and Therapeutics, Porto Alegre, Brazil
M. Migliorini Parisi
Affiliation:
Universidade Federal do Rio Grande do Sul, Postgraduate Program: Biochemistry, Porto Alegre, Brazil
M. Becker
Affiliation:
Universidade Federal do Rio Grande do Sul, Laboratory of Molecular Biology and Bioinformatics, Porto Alegre, Brazil
B.M. Ascoli
Affiliation:
Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Psiquiatria e Ciências do Comportamento, Porto Alegre, Brazil
G. Fries
Affiliation:
Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Psiquiatria e Ciências do Comportamento, Porto Alegre, Brazil
M. Kauer-Sant’anna
Affiliation:
Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Psiquiatria e Ciências do Comportamento, Porto Alegre, Brazil
F. Kapczinski
Affiliation:
Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Psiquiatria e Ciências do Comportamento, Porto Alegre, Brazil
F. Klamt
Affiliation:
Universidade Federal do Rio Grande do Sul, Department of Biochemistry, Porto Alegre, Brazil
F. T.C.R. Guma
Affiliation:
Universidade Federal do Rio Grande do Sul, Postgraduate Program: Biochemistry, Porto Alegre, Brazil
A. Ribeiro Rosa
Affiliation:
Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Psiquiatria e Ciências do Comportamento, Porto Alegre, Brazil
F.M. Barbé-Tuana
Affiliation:
Universidade Federal do Rio Grande do Sul, Postgraduate Program: Biochemistry, Porto Alegre, Brazil

Abstract

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Introduction

Evidence has suggested that immune imbalance is involved with bipolar disorder (BD); however, its precise mechanism is poorly understood.

Objective

This study investigated whether biochemical changes in the serum from BD patients could modulate the phenotype of macrophages.

Methods

Eighteen subjects with BD and healthy individuals (n = 5) were included in this study. The human monocyte cell line U-937 was activated with PMA (phorbol 12-myristate 13-acetate) and polarization was induced with RPMI-1640 media supplemented with 10% serum from each patient for 24 h. Gene expression of selected M1 and M2 markers was assessed by qPCR.

Results

Macrophages exposed to serum of manic and depressive BD patients displayed an increase of IL-1β (6.40 ± 3.47 and 9.04 ± 5.84 versus 0.23 ± 0.11; P < 0.05) and TNF-α (2.23 ± 0.91 and 2.03 ± 0.45 versus 0.62 ± 0.24; P = 0.002 and P = 0.004, respectively) compared to remitted group. In parallel, U-937 macrophages treated with serum of patients in acute episode displayed a down-regulation of CXCL9 (0.29 ± 0.20 versus 1.86 ± 1.61; P = 0.006) and CXCL10 expression (0.36 ± 0.15 and 0.86 ± 0.24 versus 1.83 ± 0.88; P < 0.000 and P = 0.04) compared to remitters.

Conclusions

Our results are consistent with previous studies showing that changes in peripheral blood markers could modulate M1/M2 polarization in BD. The evidence of macrophages as source of inflammatory cytokines might be helpful to unravel how the mononuclear phagocyte system can be involved in the etiology of BD.

Disclosure of interest

The authors have not supplied their declaration of competing interest.

Type
e-Poster walk: Anxiety disorders and somatoform disorders
Information
European Psychiatry , Volume 41 , Issue S1: Abstract of the 25th European Congress of Psychiatry , April 2017 , pp. S114
Copyright
Copyright © European Psychiatric Association 2017
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