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Protective Effect of Saikosaponin B On Damage of Cultured Sh-sy5y Cells in Vitro

Published online by Cambridge University Press:  15 April 2020

Y. Zhang ,
Q. Wu ,
F. Liu  and
T. Xu
Y. Zhang
Affiliation:
Laboratory, Xi'an mental health center, Xi'an, China
Q. Wu
Affiliation:
office, Xi'an mental health center, Xi'an, China
F. Liu
Affiliation:
science and education department, Xi'an mental health center, Xi'an, China
T. Xu
Affiliation:
student, Shaanxi university of chinese medicine, Xi'an, China

Abstract

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Introduction

Saikosaponin B is one of the main ingredients of Bupleurum. Among the many effects of Bupleurum, saikosaponin B may be contributing molecules.human neuroblastoma cell line SH-SY5Y is a tumor cells of low degree of differentiation. Its cell morphology, physiology and biochemical functions similar to normal nerve cells, are widely used to study the mechanism of diseases and drug, of the nervous system.

Objective

To investigate the effect of Saikosaponin B on SH-SY5Y cells.

Methods

Cultured SH-SY5Y cells and drawed cell growth curve. Then based on the cell growth curve, using hydrogen peroxide of different doses(110?120?130?140?150?160?180?200?220μmol/L) to treated SH-SY5Y cells. At same time, volume fraction 0.05 serum contained Saikosaponin B was added. Cultured SH-SY5Y cells were observed by morphology and tested by the MTT assay.

Results

Less than 140μmol/L hydrogen peroxide, SH-SY5Y cells does not be caused damage. Saikosaponin B of volume fraction 0.05 can relieve the damage of SH-SY5Y cells treated with 140μmol/L hydrogen peroxide, also can increase the survival of the SH-SY5Y cells.

Conclusion

Saikosaponin B can strongly protect the cultured SH-SY5Y cells from damage induced by hydrogen peroxide.

Keywords

Saikosaponin Bhydrogen peroxideSH-SY5Y cellsmorphologyMTT assay
Type
Article: 1616
Information
European Psychiatry , Volume 30 , Issue S1: Abstracts of the 23rd European Congress of Psychiatry , March 2015 , pp. 1
Copyright
Copyright © European Psychiatric Association 2015
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