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328 – Fuction of GRP78 in Hippocampus in a Rat Model of Posttraumatic Stress Disorder

Published online by Cambridge University Press:  15 April 2020

F. Han  and
Y. Shi
F. Han
Affiliation:
China Medical University, Shenyang, China
Y. Shi
Affiliation:
China Medical University, Shenyang, China

Abstract

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The mechanism of PTSD is not fully understood until now. Previous studies showed PTSD induced endoplasmic reticulum stress (ERS). Grp 78 plays important role in ERS.

Objectives:

To explore changes of Grp78 and Ca2+-CaM-CaMKIIα in hippocampus of PTSD rats may reveal part of the pathogensis.

Methods:

The models of PTSD were created by SPS, which is an established animal model for PTSD. Wistar rats were selected for this study and randomly divided into a normal control group and SPS groups of 1d, 4d and 7d. The expression of GRP78 was examined by immunofluorescence, western blotting and RT-PCR. The intracellular free calcium level was examined by fluorescence spectrophotometer. CaM and CaMKIIα were examined by RT-PCR. Apoptosis was examined by TUNEL and TEM.

Results:

The results showed the intracellular free calcium level reached the peak 1 day after SPS stimulation, then gradually decreased to normal level. The expression of CaM 1 day after SPS is the most and then decreased. CaMKIIα expression showed a significant down-regulation 1 day after SPS throughout and then gradually increased to normal level. Grp 78 reached the peak 4 day after SPS stimulation. TUNEL-positive cells significantly more than the normal control group and peaked at 7d after SPS stimuli, then gradually decreased to normal level. Furthermore, some cells had a characteristics change, including chromatin condensation, appearance of chromatin crescents, and nucleus fragmentation.

Conclusions:

The results suggest that Grp 78 and changes of Ca2+-CaM-CaMKIIα in hippocampal might play an important role in the pathology of PTSD.

Type
Abstract
Information
European Psychiatry , Volume 28 , Issue S1: Abstracts of the 21th European Congress of Psychiatry , 2013 , 28-E21
Copyright
Copyright © European Psychiatric Association 2013
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