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1175 – The Effect Of Mk-801 On The Erk-creb Signal System In Prenatally Stressed Offspring Rats

Published online by Cambridge University Press:  15 April 2020

L. Guan ,
X. Zhao ,
X. Zhang ,
H. Sun ,
D. Wang ,
Q. Su ,
G. Tang ,
L. Yang ,
Q. Song  and
N. Jia
...Show all authors
L. Guan
Affiliation:
Department of Pharmacology, Xi’an Jiaotong University, Xi’an, China
X. Zhao
Affiliation:
Department of Pharmacology, Xi’an Jiaotong University, Xi’an, China
X. Zhang
Affiliation:
Department of Pharmacology, Xi’an Jiaotong University, Xi’an, China
H. Sun
Affiliation:
Department of Pharmacology, Xi’an Jiaotong University, Xi’an, China
D. Wang
Affiliation:
Department of Biology, Northwest University, Xi’an, China
Q. Su
Affiliation:
Department of Neonatology, Xi’an, China
G. Tang
Affiliation:
Department of Biology, Northwest University, Xi’an, China
L. Yang
Affiliation:
Department of Neonatology, Xi’an, China
Q. Song
Affiliation:
Department of Biology, Northwest University, Xi’an, China
N. Jia
Affiliation:
Department of Human Anatomy and Embryology, Xi’an Jiaotong University, Xi’an, China
H. Li
Affiliation:
Department of Neonatology, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, China
Z. Zhu
Affiliation:
Department of Pharmacology, Xi’an Jiaotong University, Xi’an, China Department of Biology, Northwest University, Xi’an, China

Abstract

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Introduction

Studies revealed that prenatal stress (PS) may increase the vulnerability to depression in their offspring, and ERK-CREB signal system might play a role in its mechanism.

Objectives and aims

The present study investigated the effect of MK-801 on depressive-like behavior and its impacts on ERK2, CREB, Bcl-2 mRNA expression in PS female rat offspring.

Methods

The pregnant rats were randomly divided into three groups, the control group (Con) was left undisturbed, the PS-saline group (PS-saline) and the PS-MK-801 group (PS-MK-801) were subjected to restraint stress on days 14–20 of pregnancy three times daily for 45 min, and received an i.p. administration of saline or MK-801(sigma, 0.2 mg/kg) 30 min before the first stress respectively. Forced swimming test was undertaken to assess depressive-like behavior in one month female offspring. ERK2, CREB, Bcl-2 mRNA in the hippocampus, frontal cortex, and striatum were detected by RT-PCR.

Results

PS-saline spent significantly more immobile time compared to Con and PS-MK-801 (P < 0.05). ERK2 and CREB mRNA expression in hippocampus and frontal cortex was significantly decreased in PS-saline compared to Con and PS-MK-801 (P < 0.05), while in striatum CREB mRNA expression in PS-saline was lower than Con (P < 0.05). Bcl-2 mRNA expression in hippocampus and striatum was significantly decreased in PS-saline (P < 0.05), and in frontal cortex, its expression was significantly lower in PS-saline and PS-MK-801 (P < 0.05).

Conclusions

PS may suppress ERK-CREB signal pathway in female offspring rats, which could be partly prevented by MK- 801. (Supported by National Natural Science Foundation of China, No: 30970952).

Type
Abstract
Information
European Psychiatry , Volume 28 , Issue S1: Abstracts of the 21th European Congress of Psychiatry , 2013 , 28-E549
Copyright
Copyright © European Psychiatric Association 2013
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