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Methohexital affects neutrophil (PMN) dynamic free amino acid pool and immune functions in vitro

Published online by Cambridge University Press:  16 August 2006

J. Mühling ,
M. Fuchs ,
A. Sablotzki ,
M. Krüll ,
H. P. Ogal ,
S. Weiss ,
M. G. Dehne ,
J. Engel  and
G. Hempelmann
J. Mühling
Affiliation:
Department of Anaesthesiology and Intensive Care Medicine, Justus-Liebig-University, Giessen
M. Fuchs
Affiliation:
Dr Ing. Herbert Knauer GmbH, Berlin
A. Sablotzki
Affiliation:
Clinics of Anaesthesiology and Intensive Care Medicine, Martin-Luther-University, Halle, Wittenberg
M. Krüll
Affiliation:
Department of Internal Medicine/Infectious Diseases, Charité , Medical School of Humboldt-University, Berlin, Germany
H. P. Ogal
Affiliation:
Department of Anaesthesiology and Intensive Care Medicine, Justus-Liebig-University, Giessen
S. Weiss
Affiliation:
Department of Anaesthesiology and Intensive Care Medicine, Justus-Liebig-University, Giessen
M. G. Dehne
Affiliation:
Department of Anaesthesiology and Intensive Care Medicine, Justus-Liebig-University, Giessen
J. Engel
Affiliation:
Department of Anaesthesiology and Intensive Care Medicine, Justus-Liebig-University, Giessen
G. Hempelmann
Affiliation:
Department of Anaesthesiology and Intensive Care Medicine, Justus-Liebig-University, Giessen
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Abstract

Background and objective The objective of this study was to determine the dose as well as the duration of exposure–dependent effects of methohexital on neutrophil [polymorphonuclear leucocyte (PMN)] free amino acid profiles and, in a parallel study, on PMN immune functions.

Methods Whole blood samples were taken from 20 volunteers and incubated with methohexital [0 (control), 3.6, 26, 130 and 260 μgmL−1] for 10, 30, 60 or 120 min. PMN amino acid profiles were documented using advanced PMN separation and highperformance liquid chromatography procedures. Superoxide anion (O2−) and hydrogen peroxide production (H2O2), and activity of released myeloperoxidase (MPO), were determined photometrically.

Results After methohexital, significant dose (≥26 μg mL−1) as well as duration of exposure–dependent (≥30 min) increases in histidine, isoleucine, leucine, valine, methionine, serine, glycine, threonine, and decreases in glutamine, glutamate, aspartate, asparagine, arginine, ornithine, citrulline, alanine and taurine were observed (P ≤0.05). Concerning PMN immune functions, methohexital significantly decreased O2−, H2O2 formation and MPO (≥26 μgmL−1, ≥30 min, P≤0.05).

Conclusions Altogether, there is significant relevance to the pharmacological regimens which enhance the supply of methohexital in whole blood. In regards to our results, we suggest that considerable changes in PMN ‘dynamic free amino acid pool’, for example induced by methohexital, may be one of the determinants in cell nutrition adversely affecting PMN metabolism. It is partially through its effect on the PMN free amino acid pool that maleficent pharmacological stress may have an unintentional influence on PMN immune functions.

Keywords

amino acidsanaesthetics intravenousmethohexitalbarbituratesmethohexitalenzymesoxidoreductasesperoxidasesperoxidasefree radicalssuperoxidesimmune systemphagocytesneutrophilsleukocyesgranulocytesneutrophilsperoxidessuperoxideshydrogen peroxide
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 18 , Issue 6 , June 2001 , pp. 366 - 376
Copyright
2001 European Society of Anaesthesiology

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