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Association between GSTP1 gene polymorphism and serum α-GST concentrations undergoing sevoflurane anaesthesia

Published online by Cambridge University Press:  01 March 2008

C. Kaymak ,
B. Karahalil ,
N. N. Ozcan  and
D. Oztuna
C. Kaymak*
Affiliation:
Kirikkale University, Faculty of Medicine, Department of Anaesthesiology and Reanimation, Kirikkale
B. Karahalil
Affiliation:
University of Gazi, Faculty of Pharmacy, Department of Toxicology, Ankara
N. N. Ozcan
Affiliation:
Ministry of Health, Ankara Training and Research Hospital, Department of Anaesthesiology and Reanimation, Ankara
D. Oztuna
Affiliation:
University of Ankara, Faculty of Medicine, Department of Biostatistics, Ankara, Turkey
*
Correspondence to: Cetin Kaymak, Department of Anaesthesiology and Reanimation, Medical Faculty, Kirikkale University, Kirikkale 71100, Turkey. E-mail: [email protected]; Tel: +90 3182252485; Fax: +90 3182244697
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Summary

Background and objectives

The measurement of α-glutathione-S-transferase enzyme is one of the most sensitive indicators of hepatocellular function. Variation in the glutathione-S-transferase P1 gene clusters has been intensively investigated and polymorphism has been described. The aim of the study was to assess whether an association exists between glutathione-S-transferase P1 gene polymorphism and serum α-glutathione-S-transferase concentrations for the first postoperative day in patients who underwent anaesthesia with sevoflurane.

Methods

In all, 54 unrelated patients were enrolled in this study. Anaesthesia was induced with thiopental and fentanyl. Vecuronium was used for neuromuscular relaxation before endotracheal intubation. Anaesthesia was maintained with sevoflurane in a gas mixture containing 50% nitrous oxide in oxygen. Peripheral venous blood samples to determine serum α-glutathione-S-transferase concentrations were collected before induction (T1), at the end of anaesthesia (T2) and at 24-h postoperatively (T3). Enzyme-linked immunosorbent assay (ELISA) immunoassay was used to measure α-glutathione-S-transferase levels. Genomic DNA was isolated from serum samples using a genomic DNA purification kit. In order to detect the variants of glutathione-S-transferase P1, polymerase chain reaction – restriction fragment length polymorphism analysis was employed.

Results

Early postoperative serum α-glutathione-S-transferase levels for all patients were significantly increased when compared with preanaesthetic and 24-h postoperatively (P < 0.05). Serum α-glutathione-S-transferase concentrations, in individuals with glutathione-S-transferase P1 Ile105Val genotypes (heterozygote gene), remained elevated at 24 h (P < 0.05), whereas levels in individuals with glutathione-S-transferase P1 Ile105Ile (wild gene) decreased (P > 0.05).

Conclusions

Although α-glutathione-S-transferase levels were elevated in all patients after sevoflurane anaesthesia, levels remained high at 24 h in patients with glutathione-S-transferase P1 Ile105Val genotypes compared to controls.

Keywords

GLUTATHIONE S-TRANSFERASEGSTP1 POLYMORPHISMANAESTHESIA INHALATIONALSEVOFLURANE
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 25 , Issue 3 , March 2008 , pp. 193 - 199
Copyright
Copyright © European Society of Anaesthesiology 2008

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References

1.Hussey, AJ, Aldrige, LM, Paul, D, Ray, DC, Beckett, GJ, Allan, LG. Serum glutathione-S-transferase concentration as a measure of hepatocellular integrity following a single general anesthetic with halothane, enflurane or isoflurane. Br J Anaesth 1988; 60: 130–135.CrossRefGoogle ScholarPubMed
2.Hussey, AJ, Howie, J, Allan, LG et al. . Impaired hepatocellular integrity during general anesthesia, as assessed by measurement of serum glutathione-S-transferase. Clin Chim Acta 1986; 161: 19–28.CrossRefGoogle Scholar
3.Ray, DC, Bomont, R, Mizushima, A et al. . Effect of sevoflurane on serum concentrations of glutathione-S-transferase. Br J Anaesth 1996; 77: 404–407.CrossRefGoogle Scholar
4.Kenna, JG, Jones, RM. The organ toxicity of inhaled anesthetics. Anesth Analg 1995; 81: 51–66.CrossRefGoogle ScholarPubMed
5.Hayes, JD, Flanagen, JU, Jowsey, IR. Glutathione transferases. Ann Rev Pharmacol Toxicol 2005; 45: 51–88.CrossRefGoogle ScholarPubMed
6.Yin, Z, Ivanov, VN, Habelhah, H, Tew, K, Ronai, Z. glutathione-S-transferase p elicits protection against H2O2-induced cell death via coordinated regulation of stress kinases. Cancer Res 2000; 60: 4053–4057.Google ScholarPubMed
7.Ruscoe, JE, Rosario, LA, Wang, T et al. . Pharmacologic or genetic manipulation of glutathione-S-transferase P1-1 (GSTpi) influences cell proliferation pathways. Pharmacol Exp Ther 2001; 298: 339–345.Google ScholarPubMed
8.Gudmundsdottir, K, Tryggvadottir, L, Eyfjord, JE. GSTM1, GSTT1, and GSTP1 genotypes in relation to breast cancer risk and frequency of mutations in the p53 gene. Cancer Epidemiol Biomarkers Prev 2001; 10: 1169–1173.Google ScholarPubMed
9.Ryberg, D, Skaug, V, Hewer, A et al. . Genotypes of glutathione transferase M1 and P1 and their significance for lung DNA adduct levels and cancer risk. Carcinogenesis 1997; 18: 1285–1289.CrossRefGoogle ScholarPubMed
10.Forsberg, L, de Faire, U, Morgenstern, R. Oxidative stress, human genetic variation, and disease. Arch Biochem Biophys 2001; 389: 84–93.CrossRefGoogle ScholarPubMed
11.Strange, RC, Lear, JT, Fryer, AA. glutathione-S-transferase polymorphisms: Influence on susceptibility to cancer. Chem. Biol Interact 1998; 111: 351–364.CrossRefGoogle ScholarPubMed
12.Burim, RV, Canallle, R, Martinelli, LC, Takahashi, CS. Polymorphisms in glutathione-S-transferases GSTM1, GSTT1 and GSTP1 and cytochromes P450 CYP2E1 and CYP1A1 and susceptibility to cirrhosis or pancreatitis in alcoholics. Mutagenesis 2004; 19: 291–298.CrossRefGoogle ScholarPubMed
13.Zhong, S, Zhou, SF, Chen, X et al. . Relationship between genotype and enzyme activity of glutathione-S-transferases M1 and P1 in Chinese. Eur J Pharm Sci 2006; 28: 77–85.CrossRefGoogle ScholarPubMed
14.Higuchi, H, Adachi, Y, Wada, H, Kanno, M, Satoh, T. Comparison of serum α glutathione-S-transferase concentrations during after low-flow sevoflurane or isoflurane anesthesia. Acta Anaesthesiol Scand 2001; 45: 1226–1229.CrossRefGoogle Scholar
15.Darling, JR, Murray, JM, McBride, DR, Trinick, TR, Fee, JP. Serum glutathione-S-transferase concentrations and creatinine clearance after sevoflurane anaesthesia. Anaesthesia 1997; 52: 121–126.CrossRefGoogle ScholarPubMed
16.Hayes, JD, McLellan, LI. Glutathione and glutathione-dependent enzymes represent a co-ordinately regulated defense against oxidative stress. Free Radic Res 1999; 31: 273–300.CrossRefGoogle ScholarPubMed
17.Strange, RC, Spiteri, MA, Ramachandran, S, Fryer, AA. glutathione-S-transferase family of enzymes. Mutat Res 2001; 482: 21–26.CrossRefGoogle ScholarPubMed
18.Adler, V, Yin, Z, Fuchs, SY et al. . Regulation of JNK signaling by GSPpi. EMBO J 1999; 18: 1321–1334.CrossRefGoogle Scholar
19.Redick, JA, Jakoby, WB, Baron, J. Immunohistochemical localization of glutathione-S-transferase in liver of untreated rats. J Biol Chem 1982; 257: 15200–15203.CrossRefGoogle ScholarPubMed
20.Sundberg, AGM, Nilsson, R, Appelkvist, EL, Dallner, G. Immunohistochemical localization of alpha and pi class glutathione transferases in human tissue. Pharm Tox 1993; 72: 321–331.CrossRefGoogle Scholar
21.Trull, AK, Facey, SP, Rees, GW et al. . Serum glutathione-S-transferase – a sensitive marker of hepatocellular damage associated with acute liver allograft rejection. Transplantation 1994; 58: 1345–1351.Google ScholarPubMed
22.Johansson, AS, Stenberg, G, Widersten, M et al. . Structure-activity relationship and thermal stability of human glutathione transferase P1-1 governed by the H-site residue 105. J Mol Biol 1998; 278: 687–698.CrossRefGoogle ScholarPubMed
23.Alves-Silva, J, da Silva Santos, M, Guimaraes, PE et al. . The ancestry of Brazilian mtDNA lineages. Am J Hum Genet 2000; 67: 444–461.CrossRefGoogle ScholarPubMed
24.Watson, MA, Stewart, RK, Smith, GB, Massey, TE, Bell, DA. Human glutathione-S-transferase P1 polymorphisms: relationship to lung tissue enzyme activity and population frequency distribution. Carcinogenesis 1998; 19: 275–280.CrossRefGoogle ScholarPubMed
25.Ghobadloo, SM, Yaghmaei, B, Bakayev, V et al. . GSTP1, GSTM1, and GSTT1 genetic polymorphisms in patients with cryptogenic liver cirrhosis. J Gastrointest Surg 2004; 8: 423–427.CrossRefGoogle ScholarPubMed
26.Frink, EJ, Morgan, SE, Coetzee, A, Contzen, F, Jr.Brown, BR. The effects of sevoflurane, halothane, enflurane and isoflurane on hepatic blood flow and oxygenation in chronically instrumented greyhound dogs. Anesthesiology 1992; 76: 85–90.CrossRefGoogle ScholarPubMed
27.Conzen, PF, Vollmar, B, Habazettl, H et al. . Systemic and regional hemodynamics of isoflurane and sevoflurane in rats. Anesth Analg 1992; 74: 79–88.CrossRefGoogle ScholarPubMed
28.Bernard, JM, Doursout, MF, Wouters, P et al. . Effects of sevoflurane and isoflurane on hepatic circulation in the chronically instrumented dog. Anesthesiology 1992; 77: 541–545.CrossRefGoogle ScholarPubMed
29.Hongo, T. Sevoflurane reduced but isoflurane maintained hepatic blood flow during anesthesia in man. J Anesth 1994; 8: 55–59.CrossRefGoogle ScholarPubMed
30.Nishiyama, T, Yokoyama, T, Hanaoka, K. Effects of sevoflurane and isoflurane anesthesia on arterial ketone body ratio and liver function. Acta Anaesth Scand 1999; 43: 347–351.CrossRefGoogle ScholarPubMed
31.Kanaya, N, Nakayama, M, Fujita, S, Namiki, A. Comparison of the effects of sevoflurane, isoflurane and halothane on indocyanine green clearance. Br J Anaesth 1995; 74: 164–167.CrossRefGoogle ScholarPubMed
32.Kobayashi, M. the effects of sevoflurane and isoflurane on hepatic blood flow in man. Masui 1996; 45: 281–286.Google ScholarPubMed
33.Crawford, MW, Lerman, J, Saldivia, V, Orrego, H, Carmichael, FJ. The effects of adenosine-induced hypotension on systemic and splanchnic hemodynamics during halothane or sevoflurane anesthesia in the rat. Anesthesiology 1994; 80: 159–167.CrossRefGoogle ScholarPubMed
34.Gonowski, C, Laster, M, Eger EI, II, Ferelı, L, Kerschmann, R. Toxicity of compound A in rats: effect of a three-hour administration. Anesthesiology 1994; 80: 556–565.CrossRefGoogle Scholar
35.Frink, EJ. The hepatic effects of sevoflurane. Anesth Analg 1995; 81: 46–50.CrossRefGoogle ScholarPubMed