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Changes in plasma cortisol and ascorbic acid in horses with and without recurrent airway obstruction upon exercise and ascorbic acid supplementation

Published online by Cambridge University Press:  09 March 2007

TL Cuff ,
RJ Williams ,
CM Deaton ,
NC Smith ,
BD Davies ,
MCG Davies-Morel ,
DJ Marlin  and
PA Harris
TL Cuff*
Affiliation:
Hartpury College, Hartpury House, Gloucester GL19 3BE, UK Centre for Equine Studies, Animal Health Trust, Lanwades Park, Kentford, Suffolk CB8 7UU, UK
RJ Williams
Affiliation:
Hartpury College, Hartpury House, Gloucester GL19 3BE, UK
CM Deaton
Affiliation:
Centre for Equine Studies, Animal Health Trust, Lanwades Park, Kentford, Suffolk CB8 7UU, UK
NC Smith
Affiliation:
Centre for Equine Studies, Animal Health Trust, Lanwades Park, Kentford, Suffolk CB8 7UU, UK
BD Davies
Affiliation:
The Institute of Rural Sciences, University of Wales, Llanbadarn Fawr, Aberystwyth, Ceredigion SY23 3AL, UK
MCG Davies-Morel
Affiliation:
The Institute of Rural Sciences, University of Wales, Llanbadarn Fawr, Aberystwyth, Ceredigion SY23 3AL, UK
DJ Marlin
Affiliation:
Centre for Equine Studies, Animal Health Trust, Lanwades Park, Kentford, Suffolk CB8 7UU, UK
PA Harris
Affiliation:
Equine Studies Group, WALTHAM Centre for Pet Nutrition, Waltham-on-the-Wolds, Leicestershire, UK
*
*[email protected]
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Abstract

Diminished basal plasma cortisol concentrations and a blunted cortisol response to exercise have been observed in human asthmatics. In horses with recurrent airway obstruction (RAO), plasma concentrations of cortisol at rest are not significantly different from those of healthy horses, but the effect of exercise on endogenous cortisol concentrations has not been described. Ascorbic acid is a non-enzymatic antioxidant with proposed immune-modulating properties. In man, supplementation with ascorbic acid has been shown to attenuate the exercise-induced increase in plasma cortisol following prolonged, submaximal exercise. The relationship between cortisol and ascorbic acid has not previously been investigated in the horse. In a blinded cross-over design, five horses with RAO and six healthy non-RAO controls performed a standard exercise test following 4 weeks of supplementation with either an antioxidant (providing 10 mg ascorbic acid kg−1 day−1) or a placebo (<1 mg ascorbic acid kg−1 day−1). Venous blood samples were obtained 1 h prior to exercise and at 0, 15, 60 min and 24 h thereafter. Exercise resulted in a significant increase in plasma cortisol concentrations in both groups of horses (P<0.05). Basal and post-exercise concentrations of plasma cortisol in the RAO group (136±16 and 210±16 μmol l−1, respectively) were not significantly different from those in the non-RAO group (129±43 and 218±30 μmol l−1, respectively). Antioxidant supplementation increased basal and post-exercise concentrations of plasma ascorbic acid in RAO and non-RAO horses (P<0.05) but had no effect on plasma cortisol concentration in either group, before or after exercise (RAO: rest 157±27 μmol l−1, post-exercise 222±21 μmol l−1; non-RAO: rest 140±11 μmol l−1, post-exercise 227±35 μmol l−1). In conclusion, RAO-affected horses in remission demonstrate the same cortisol response to exercise as healthy controls. Antioxidant supplementation had no impact on post-exercise concentrations of plasma cortisol in either healthy or RAO-affected horses in remission.

Keywords

recurrent airway obstructionchronic obstructive pulmonary diseaseheavesrespiratory diseasevitamin Cantioxidanthydrocortisone
Type
Research Article
Information
Equine and Comparative Exercise Physiology , Volume 2 , Issue 2 , May 2005 , pp. 105 - 112
Copyright
Copyright © Cambridge University Press 2005

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References

1Thornton, JR (1985) Hormonal responses to exercise and training. Veterinary Clinics of North America. Equine Practice 1(3): 477–496.CrossRefGoogle ScholarPubMed
2Landstra, AM, Postma, DS, Boezen, HM, van Aalderen, WM (2002) Role of serum cortisol levels in children with asthma. American Journal of Respiratory and Critical Care Medicine 165(5): 708–712.CrossRefGoogle ScholarPubMed
3Kapoor, U, Tayal, G, Mittal, SK, Sharma, VK and Tekur, U (2003) Plasma cortisol levels in acute asthma. Indian Journal of Pediatrics 70(12): 965–968.CrossRefGoogle ScholarPubMed
4Tremblay, MS, Copeland, JL, Van Helder, W (2004) Effect of training status and exercise mode on endogenous steroid hormones in men. Journal of Applied Physiology 96(2): 531–539.CrossRefGoogle ScholarPubMed
5Snow, DH and Rose, RJ (1981) Hormonal changes associated with long distance exercise. Equine Veterinary Journal 13(3): 195–197.CrossRefGoogle ScholarPubMed
6Lassourd, V, Gayrard, V, Laroute, V, Alvinerie, M, Benard, P, Courtot, D et al. (1996) Cortisol disposition and production rate in horses during rest and exercise. American Journal of Physiology 271 1 Pt 2: R25–R33.Google ScholarPubMed
7Marc, M, Parvizi, N, Ellendorff, F, Kallweit, E and Elsaesser, F (2000) Plasma cortisol and ACTH concentrations in the Warmblood horse in response to a standardized treadmill exercise test as physiological markers for evaluation of training status. Journal of Animal Science 78(7): 1936–1946.CrossRefGoogle ScholarPubMed
8Williams, RJ, Marlin, DJ, Smith, N, Harris, RC, Haresign, W, Davies Morel, MC (2002) Effects of cool and hot humid environmental conditions on neuroendocrine responses of horses to treadmill exercise. Veterinary Journal 164(1): 54–63.CrossRefGoogle ScholarPubMed
9Robson, PJ, Alston, TD and Myburgh, KH (2003) Prolonged suppression of the innate immune system in the horse following an 80 km endurance race. Equine Veterinary Journal 35(2): 133–137.CrossRefGoogle ScholarPubMed
10Hoffsis, GF and Murdick, PW (1970) The plasma concentrations of corticosteroids in normal and diseased horses. Journal of the American Veterinary Medical Association 157(11): 1590–1594.Google ScholarPubMed
11Mills, PC, Ng, JC, Kramer, H and Auer, DE (1997) Stress response to chronic inflammation in the horse. Equine Veterinary Journal 29(6): 483–486.CrossRefGoogle ScholarPubMed
12Fujitaka, M, Nomura, S, Sakura, N, Ueda, K, Matuura, R and Yumiba, C (2000) Morning and afternoon serum levels of cortisone and cortisol in asthmatic patients. Clinica Chimica Acta 299 (1–2): 101–108.CrossRefGoogle ScholarPubMed
13Kallenbach, JM, Panz, V, Girson, MS, Joffe, BI and Seftel, HC (1990) The hormonal response to exercise in asthma. European Respiratory Journal 3(2): 171–175.CrossRefGoogle ScholarPubMed
14Buske-Kirschbaum, A, von Auer, K, Krieger, S, Weis, S, Rauh, W and Hellhammer, D (2003) Blunted cortisol responses to psychosocial stress in asthmatic children: a general feature of atopic disease? Psychosomatic Medicine 65(5): 806–810.CrossRefGoogle ScholarPubMed
15Sly, RM, Joseph, F and Johnson, CM (1973) Effect of exercise upon plasma cortisol and airway obstruction in asthmatic children. Annals of Allergy 31(8): 371–374.Google ScholarPubMed
16Jaffe, P, Konig, P, Ijaduola, O, Walker, S and Godfrey, S (1973) Relationship between plasma cortisol and peak expiratory flow rate in exercise-induced asthma and the effect of sodium cromoglycate. Clinical Science and Molecular Medicine 45(4): 533–541.Google Scholar
17Linden, A, Art, T, Amory, H, Desmecht, D and Lekeux, P (1991). Effect of 5 different types of exercise, transportation and ACTH administration on plasma cortisol concentration in sport horses. In: Persson, SGB, Lindholm, A & Jeffcott, LB (eds), Equine Exercise Physiology 3. Davis, CA: ICEEP Publications. pp. 391–396.Google Scholar
18Clark, DK, Friend, TH and Dellmeier, G (1993) The effect of orientation during trailer transport on heart rate, cortisol and balance in horses. Applied Animal Behaviour Science 38: 179–189.CrossRefGoogle Scholar
19Alexander, SL, Irvine, CH, Livesey, JH and Donald, RA (1988) Effect of isolation stress on concentrations of arginine vasopressin, α-melanocyte-stimulating hormone and ACTH in the pituitary venous effluent of the normal horse. Journal of Endocrinology 116(3): 325–334.CrossRefGoogle ScholarPubMed
20Bureau, F, Bonizzi, G, Kirschvink, N, Delhalle, S, Desmecht, D, Merville, MP et al. (2000) Correlation between nuclear factor-κB activity in bronchial brushing samples and lung dysfunction in an animal model of asthma. American Journal of Respiratory and Critical Care Medicine 161 (4 Pt 1): 1314–1321.CrossRefGoogle Scholar
21Picandet, V, Leguillette, R and Lavoie, JP (2003) Comparison of efficacy and tolerability of isoflupredone and dexamethasone in the treatment of horses affected with recurrent airway obstruction (“heaves”). Equine Veterinary Journal 35(4): 419–424.CrossRefGoogle ScholarPubMed
22Lapointe, JM, Lavoie, JP and Vrins, AA (1993) Effects of triamcinolone acetonide on pulmonary function and bronchoalveolar lavage cytologic features in horses with chronic obstructive pulmonary disease. American Journal of Veterinary Research 54(8): 1310–1316.CrossRefGoogle ScholarPubMed
23Rush, BR, Trevino, IC, Matson, CJ and Hakala, JE (1999) Serum cortisol concentrations in response to incremental doses of inhaled beclomethasone dipropionate. Equine Veterinary Journal 31(3): 258–261.CrossRefGoogle ScholarPubMed
24Grimble, RF (1997) Effect of antioxidative vitamins on immune function with clinical applications. International Journal for Vitamin and Nutrition Research 67(5): 312–320.Google ScholarPubMed
25Palmer, FM, Nieman, DC, Henson, DA, McAnulty, SR, McAnulty, L, Swick, NS et al. 2003) Influence of vitamin C supplementation on oxidative and salivary IgA changes following an ultramarathon. European Journal of Applied Physiology 89(1): 100–107.CrossRefGoogle ScholarPubMed
26Peters, EM, Anderson, R, Nieman, DC, Fickl, H and Jogessar, V (2001) Vitamin C supplementation attenuates the increases in circulating cortisol, adrenaline and anti-inflammatory polypeptides following ultramarathon running. International Journal of Sports Medicine 22(7): 537–543.CrossRefGoogle ScholarPubMed
27Kitabchi, AE (1967) Ascorbic acid in steroidogenesis. Nature 215(108): 1385–1386.CrossRefGoogle ScholarPubMed
28Kitabchi, AE, Nathans, AH and Kitchell, CL (1973) Adrenal gland in vitamin E deficiency. 3. Inhibition of adrenocorticotropic hormone-induced steroidogenesis in isolated adrenal cells by ascorbic acid. Journal of Biological Chemistry 248(3): 835–840.CrossRefGoogle ScholarPubMed
29Gleeson, M, Robertson, JD and Maughan, RJ (1987) Influence of exercise on ascorbic acid status in man. Clinical Science (London) 73(5): 501–505.CrossRefGoogle ScholarPubMed
30Nieman, DC, Henson, DA, McAnulty, SR, McAnulty, L, Swick, NS, Utter, AC et al. (2002) Influence of vitamin C supplementation on oxidative and immune changes after an ultramarathon. Journal of Applied Physiology 92(5): 1970–1977.CrossRefGoogle ScholarPubMed
31Enwonwu, CO, Sawiris, P and Chanaud, N (1995) Effect of marginal ascorbic acid deficiency on saliva level of cortisol in the guinea pig. Archives of Oral Biology 40(8): 737–742.CrossRefGoogle ScholarPubMed
32Pintauro, SJ and Bergan, JG (1982) Effects of ascorbic acid on in vitro steroidogenesis in guinea pigs. Journal of Nutrition 112(3): 584–591.CrossRefGoogle ScholarPubMed
33Doulas, NL, Constantopoulos, A and Litsios, B (1987) Effect of ascorbic acid on guinea pig adrenal adenylate cyclase activity and plasma cortisol. Journal of Nutrition 117(6): 1108–1114.CrossRefGoogle ScholarPubMed
34Civen, M, Leeb, JE, Wishnow, RM and Morin, RJ (1980) Effects of dietary ascorbic acid and vitamin E deficiency on rat adrenal cholesterol ester metabolism and corticosteroidgoenesis. International Journal for Vitamin and Nutrition Research 50(1): 70–78.Google ScholarPubMed
35Peters, EM and Bateman, ED (1983) Ultramarathon running and upper respiratory tract infections. An epidemiological survey. South African Medical Journal 64(15): 582–584.Google ScholarPubMed
36Peters, EM, Goetzsche, JM, Grobbelaar, B and Noakes, TD (1993) Vitamin C supplementation reduces the incidence of postrace symptoms of upper-respiratory-tract infection in ultramarathon runners. American Journal of Clinical Nutrition 57(2): 170–174.CrossRefGoogle ScholarPubMed
37Kohut, ML, Boehm, GW and Moynihan, JA (2001) Prolonged exercise suppresses antigen-specific cytokine response to upper respiratory infection. Journal of Applied Physiology 90(2): 678–684.CrossRefGoogle ScholarPubMed
38Belayat, F, Meniai, K, Michaux, C, Kafidi, N, Coignoul, F and Dewaele, A (1998) In vitro effect of glucocorticoids on phagocytic function of sheep alveolar macrophages. Veterinary Journal 155(2): 177–181.CrossRefGoogle ScholarPubMed
39Nieman, DC (2000) Exercise immunology: future directions for research related to athletes, nutrition, and the elderly. International Journal of Sports Medicine 21 (Suppl. 1): S61–S68.CrossRefGoogle ScholarPubMed
40Stull, CL and Rodiek, AV (2000) Physiological responses of horses to 24 hours of transportation using a commercial van during summer conditions. Journal of Animal Science 78(6): 1458–1466.CrossRefGoogle ScholarPubMed
41Burns, JJ (1957) Missing step in man, monkey and guinea pig required for the biosynthesis of l -ascorbic acid. Nature 180(4585): 553.CrossRefGoogle ScholarPubMed
42Snow, DH and Frigg, M (1990) Bioavailability of ascorbic acid in horses. Journal of Veterinary Pharmacology and Therapeutics 13(4): 393–403.CrossRefGoogle ScholarPubMed
43Deaton, CM, Marlin, DJ, Roberts, CA, Smith, N, Harris, PA, Kelly, FJ et al. (2002) Antioxidant supplementation and pulmonary function at rest and exercise. Equine Veterinary Journal Supplement 34 58–65.CrossRefGoogle Scholar
44Deaton, CM, Marlin, DJ, Smith, NC, Harris, PA, Roberts, CA, Schroter, RC et al. (2004) Pulmonary epithelial lining fluid and plasma ascorbic acid concentrations in horses affected by recurrent airway obstruction. American Journal of Veterinary Research 65(1): 80–87.CrossRefGoogle ScholarPubMed
45Marlin, DJ, Fenn, K, Smith, N, Deaton, CD, Roberts, CA, Harris, PA et al. (2002) Changes in circulatory antioxidant status in horses during prolonged exercise. Journal of Nutrition 132 (6 Suppl. 2): 1622S–1627S.CrossRefGoogle ScholarPubMed
46Deaton, CM, Marlin, DJ, Smith, NC, Harris, PA, Schroter, RC and Kelly, FJ (2004) Antioxidant supplementation in horses affected by recurrent airway obstruction. Journal of Nutrition 134 (8): 2065S–2067S.CrossRefGoogle ScholarPubMed
47Breuner, CW and Orchinik, M (2002) Plasma binding proteins as mediators of corticosteroid action in vertebrates. Journal of Endocrinology 175(1): 99–112.CrossRefGoogle ScholarPubMed
48Le Roux, CW, Sivakumaran, S, Alaghband-Zadeh, J, Dhillo, W, Kong, WM and Wheeler, MJ (2002) Free cortisol index as a surrogate marker for serum free cortisol. Annals of Clinical Biochemistry 39(4): 406–408.CrossRefGoogle ScholarPubMed
49Bottoms, GD, Roesel, OF, Rausch, FD and Akins, EL (1972) Circadian variation in plasma cortisol and corticosterone in pigs and mares. American Journal of Veterinary Research 33(4): 785–790.Google ScholarPubMed
50Toutain, PL, Oukessou, M, Autefage, A and Alvinerie, M (1988) Diurnal and episodic variations of plasma hydrocortisone concentrations in horses. Domestic Animal Endocrinology 5(1): 55–59.CrossRefGoogle ScholarPubMed
51Larsson, M, Edqvist, LE, Ekman, L and Persson, S (1979) Plasma cortisol in the horse, diurnal rhythm and effects of exogenous ACTH. Acta Veterinaria Scandinavica 20(1): 16–24.CrossRefGoogle ScholarPubMed
52Kumar, L, Miklich, DR and Morris, HG (1971) Plasma 17-OH corticosteroid concentrations in children with asthma. Journal of Pediatrics 79(6): 955–962.CrossRefGoogle ScholarPubMed
53Wilson, WD, Kingery, S and Snow, DH (1991). The effect of training on adrenocortical function in Thoroughbred racehorses. In: Persson, SGB, Lindholm, A & Jeffcott, LB (eds), Equine Exercise Physiology 3. Davis, CA: ICEEP Publications.Google Scholar
54Leisti, S, Finnila, MJ and Kiuru, E (1979) Effects of physical training on hormonal responses to exercise in asthmatic children. Archives of Disease in Childhood 54(7): 524–528.CrossRefGoogle ScholarPubMed
55Art, T, Kirschvink, N, Smith, N, Votion, D and Lekeux, P (1999) Cardiorespiratory measurements and indices of oxidative stress in exercising COPD horses. Equine Veterinary Journal Supplement 30: 83–87.CrossRefGoogle Scholar
56White, A, Estrada, M, Walker, K, Wisnia, P, Filgueira, G, Valdes, F et al. (2001) Role of exercise and ascorbate on plasma antioxidant capacity in thoroughbred race horses. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology 128(1): 99–104.CrossRefGoogle ScholarPubMed
57Packer, JE, Slater, TF and Willson, RL (1979) Direct observation of a free radical interaction between vitamin E and vitamin C. Nature 278(5706): 737–738.CrossRefGoogle ScholarPubMed
58Piercy, RJ, Hinchcliff, KW, DiSilvestro, RA, Reinhart, GA, Baskin, CR, Hayek, MG et al. (2000) Effect of dietary supplements containing antioxidants on attenuation of muscle damage in exercising sled dogs. American Journal of Veterinary Research 61(11): 1438–1445.CrossRefGoogle ScholarPubMed
59Singh, A, Papanicolaou, DA, Lawrence, LL, Howell, EA, Chrousos, GP and Deuster, PA (1999) Neuroendocrine responses to running in women after zinc and vitamin E supplementation. Medicine and Science in Sports and Exercise 31(4): 536–542.CrossRefGoogle ScholarPubMed
60Peake, JM (2003) Vitamin C: effects of exercise and requirements with training. International Journal of Sport Nutrition and Exercise Metabolism 13(2): 125–151.CrossRefGoogle ScholarPubMed
61Nieman, DC, Peters, EM, Henson, DA, Nevines, EI and Thompson, MM (2000) Influence of vitamin C supplementation on cytokine changes following an ultramarathon. Journal of Interferon & Cytokine Research 20(11): 1029–1035.CrossRefGoogle ScholarPubMed
62Kipp, DE and Rivers, JM (1987) Uptake and release of adrenal ascorbic acid in the guinea pig after injection of ACTH. Journal of Nutrition 117(9): 1570–1575.CrossRefGoogle ScholarPubMed
63Laney, PH, Levy, JA and Kipp, DE (1990) Plasma cortisol and adrenal ascorbic acid levels after ACTH treatment with a high intake of ascorbic acid in the guinea pig. Annals of Nutrition & Metabolism 34(2): 85–92.CrossRefGoogle ScholarPubMed
64Thompson, D, Williams, C, McGregor, SJ, Nicholas, CW, McArdle, F, Jackson, MJ et al. (2001) Prolonged vitamin C supplementation and recovery from demanding exercise. International Journal of Sport Nutrition and Exercise Metabolism 11(4): 466–481.CrossRefGoogle ScholarPubMed