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The specific IgM response to Mycoplasma pneumoniae infection: interpretation and application to early diagnosis

Published online by Cambridge University Press:  19 October 2009

J. H. Moule ,
E. O. Caul  and
T. G. Wreghitt
J. H. Moule
Affiliation:
Bristol Public Health Laboratory, Myrtle Road, Kingsdown, Bristol BS2 8EL
E. O. Caul
Affiliation:
Bristol Public Health Laboratory, Myrtle Road, Kingsdown, Bristol BS2 8EL
T. G. Wreghitt
Affiliation:
Clinical Microbiology and Public Health Laboratory, Level 6, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QW
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Specific IgM antibody production in patients with serologically proven Mycoplasma pneumoniae infection by the complement fixation (CF) test was studied using a μ-capture ELISA. Sera from 79% of patients were found to be IgM positive. Patients could be divided into two groups relating to the amount of specific antibody produced. High levels of specific IgM (≥10 units) were more commonly found in younger patients. Seventy-six per cent of patients under the age of 20 produced relatively high levels of IgM compared to 35% of patients over the age of 20. In contrast, the number of patients who produced low or undetectable levels of IgM ( < 10 units) was found to increase with age. This trend was found to be significant which suggests that low or undetectable levels of IgM may be due to reinfection with M. pneumoniae. Specific IgM was found to appear in the serum at approximately 7 days after the onset of symptoms, peaking at between 10 and 30 days, and then falling to undetectable levels at an estimated 12–26 weeks post onset of symptoms. Twenty-eight per cent of acute-phase sera (CF titres < 256) from patients whose sera subsequently showed a fourfold or greater rise in M. pneumoniae CF antibody titre were IgM positive. Thus using μ-capture ELISA a diagnosis of M. pneumoniae infection may often be made more rapidly than by the complement fixation test.

Type
Research Article
Information
Epidemiology & Infection , Volume 99 , Issue 3 , December 1987 , pp. 685 - 692
Copyright
Copyright © Cambridge University Press 1987

References

REFERENCES

Biberfeld, G. (1971). Antibody responses in Mycoplasma pneumoniae infection in relation to serum immunoglobulins, especially Igm. Acta Pathologica et Microbiologica Scandinavica, section B 79, 620–634.Google Scholar
Bradstreet, C. M. P. & Taylor, C. E. D. (1962). Technique of complement-fixation test applicable to the diagnosis of virus diseases. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 21, 96–104.Google Scholar
Chamberlain, P. & Saeed, A. A. (1983). A study of the specific Igm antibody response in Mycoplasma pneumoniae infection in man. Journal of Hygiene 90, 207–211.CrossRefGoogle ScholarPubMed
Chanock, R. M., Hayflick, L. & Barile, M. D. (1962). Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proceedings of the National Academy of Sciences of the United States of America 48, 41–49.CrossRefGoogle ScholarPubMed
Dussaix, E., Slim, A. & Tournier, P. (1983). Comparison of enzyme-linked immunosorbent assay (ELISA) and complement fixation test for detection of Mycoplasma pneumoniae antibodies. Journal of Clinical Pathology 36, 228–232.CrossRefGoogle ScholarPubMed
Forsyth, B. R. & Chanock, R. M. (1966). Mycoplasma pneumonia. Annual Review of Medicine 17, 371–382.CrossRefGoogle ScholarPubMed
Foy, H. M., Kenny, G. E., McMahan, R., Mansy, A. M. & Grayston, J. T. (1970). Mycoplasma pneumoniae pneumonia in an urban area. Journal of the American Medical Association 214, 1666–1672.CrossRefGoogle Scholar
Foy, H. M., Kenny, G. E., Sefi, R., Ochs, H. D. & Allan, I. D. (1977). Second attacks of pneumonia due to Mycoplasma pneumoniae. Journal of Infectious Diseases 135, 673–677.CrossRefGoogle ScholarPubMed
Foy, H. M., Kenny, G. E., Cooney, M. K., Allan, I. D. & Van Belle, G. (1983). Naturally acquired immunity to pneumonia due to Mycoplasma pneumoniae. Journal of Infectious Diseases 147, 967–973.CrossRefGoogle ScholarPubMed
Hu, P. C., Powell, D. A., Albright, F., Gardner, D. E., Collier, A. M. & Clyde, W. A. (1983). A solid-phase radioimmunoassay for detection of antibodies against Mycoplasma pneumoniae. Journal of Clinical and Laboratory Immunology 11, 209–213.Google ScholarPubMed
Leinikki, P. O., Panzar, P. & Tykka, H. (1978). Immunoglobulin M antibody response against Mycoplasma pneumoniae lipid antigen in patients with acute pancreatitis. Journal of Clinical Microbiology 8, 113–118.CrossRefGoogle ScholarPubMed
Nakamura, S., Ebisawa, L., Kitamoto, O. & Sato, T. (1970). Persistence of serum antibody following Mycoplasma pneumoniae infection. American Review of Respiratory Diseases 101, 620–622.Google ScholarPubMed
Peto, R., Pike, M. C., Armitage, P., Breslow, N. E., Cox, D. R., Howard, S. V., Mantel, N., McPherson, K., Peto, J. & Smith, P. G. (1977). Design and analysis of randomized clinical trials which require prolonged observations of each patient. 2. Analysis and examples. British Journal of Cancer 35, 1–39.CrossRefGoogle Scholar
Price, P. C. (1980). Direct radioimmunoassay for the detection of Igm antibodies against Mycoplasma pneumoniae. Journal of Immunological Methods 32, 261–273.CrossRefGoogle ScholarPubMed
Raisanen, S. M., Suni, J. & Leinikki, P. (1980). Serological diagnosis of Mycoplasma pneumoniae infection by enzyme immunoassay. Journal of Clinical Pathology 33, 836–840.CrossRefGoogle ScholarPubMed
Sillis, M. & Andrews, B. (1978). A simple test for Mycoplasma pneumoniae Igm. Zentralblatt für Bakteriologie, Mirobiologie, Infektionskrankheiten und Hygiene, Abteilung 1: Originate, Reihe A: Medizinische Mikrobiologie und Parasitologie 241, 239–240.Google Scholar
Skaug, K., Eng, J., Orstavik, I. & Haug, K. W. (1976). The diagnostic value of determination of Igm antibodies against Mycoplasma pneumoniae infections by the indirect immunofluorescent antibody test. Acta Pathologica et Microbiologica Scandinavica, section B 84, 170–176.Google ScholarPubMed
Watson, G. I. (1977). The treatment of Mycoplasma pneumoniae infections. Scottish Medical Journal 22, 331–335.CrossRefGoogle ScholarPubMed
Wreghitt, T. G. & Sillis, M. (1985). A μ-capture ELISA for detecting Mycoplasma pneumoniae Igm: comparison with indirect immunofluorescence and indirect ELISA. Journal of Hygiene 94, 217–227.CrossRefGoogle ScholarPubMed