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Soluble antigens obtained from influenza virus by treatment with non-ionic detergent

Published online by Cambridge University Press:  15 May 2009

M. J. Corbel  and
C. J. M. Rondle
M. J. Corbel
Affiliation:
Department of Bacteriology and Immunology, London School of Hygiene and Tropical Medicine, Keppel Street, W.C. 1
C. J. M. Rondle
Affiliation:
Department of Bacteriology and Immunology, London School of Hygiene and Tropical Medicine, Keppel Street, W.C. 1
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Highly purified influenza virus was degraded using anionic and non-ionic detergents. Best results were obtained using the non-ionic detergent Triton N101. Tests showed that virus extracts contained neuraminidase and a substance that reacted specifically with rabbit antibody to virus haemagglutinin (specific serum blocking substance). Haemagglutination-inhibiting antibody was produced when virus extracts were inoculated into guinea-pigs. Immunodiffusion tests showed that extracts were complex. Host-specific material was regularly found. Under appropriate conditions S-antigen was detected as a single line pattern component. Two or more virus-specific materials were also present. One of these was probably neuraminidase and the other the specific serum blocking substance.

Type
Research Article
Information
Journal of Hygiene , Volume 68 , Issue 1 , March 1970 , pp. 81 - 96
Copyright
Copyright © Cambridge University Press 1970

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