Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-10-31T22:01:47.654Z Has data issue: false hasContentIssue false
  • English
  • Français

Solid-phase radioimmunoassay techniques for the detection of African swine fever antigen and antibody

Published online by Cambridge University Press:  15 May 2009

J. R. Crowther ,
R. C. Wardley  and
P. J. Wilkinson
J. R. Crowther
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey, U.K.
R. C. Wardley
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey, U.K.
P. J. Wilkinson
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey, U.K.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A solid phase radioimmunoassay (RIA) has been successfully developed to measure both African swine fever virus (ASFV) antigen and antibody. Studies show that the assay is reproducible and will detect limiting antigen concentrations equivalent to 50–500 HAD50/ml. Both direct and indirect antibody RIA have been developed and have proved to be approximately 100 times more sensitive than the complement fixation test at present available and 1000 times more sensitive than the immuno-electro-osmophoresis test for the detection of ASFV antibody.

Type
Research Article
Information
Journal of Hygiene , Volume 83 , Issue 2 , October 1979 , pp. 353 - 361
Copyright
Copyright © Cambridge University Press 1979

References

Akao, Y., Sano, R., Sugiwa, A. & Shimojo, H. (1962). Procomplementary activity of swine serum and its elimination with periodate treatment. Japanese Journal of Medical Science and Biology 15, 165–73.CrossRefGoogle ScholarPubMed
Almeida, J. D., Waterson, A. P. & Plowright, W. (1967). The morphological characteristics of African swine fever virus and its resemblance to Tipula iridescent virus. Archiv für die gesamte Virusforschung 20, 392–6.Google Scholar
Fahey, J. L. & Terry, E. W. (1973). Ion exchange chromatography and gel filtration. In Immunochemistry, 2nd ed., Vol. 1, Ch. 7. Oxford: Blackwell Publications.Google Scholar
Gilman, S. C. & Docherty, J. J. (1977). Detection of antibodies specific for Herpes Simplex virus in human sera by the enzyme linked immunosorbent assay. Journal of Infectious Diseases 136, 286–93.Google Scholar
Hess, W. R. (1971). African swine fever virus. Virology Monographs 9, 1–33.Google Scholar
Hess, W. R., Cox, B. F., Heuschele, W. P. & Stone, S. S. (1965). Propagation and modification of African swine fever virus in cell cultures. American Journal of Veterinary Research 26, 141–6.Google Scholar
Heuschele, W. P., Coggins, L. & Stone, S. S. (1966). Fluorescent antibody studies on African swine fever virus. American Journal of Veterinary Research 27, 477–84.Google ScholarPubMed
Malmquist, W. A. & Hay, D. (1960). Haemadsorption and cytopathic effect produced by African swine fever virus in swine bone marrow and buffy coat cultures. American Journal of Veterinary Research 21, 104–8.Google Scholar
Montgomery, R. E. (1921). On a form of swine fever occurring in British East Africa (Kenya Colony). Journal of Comparative Pathology 34, 159–91, 243–62.Google Scholar
Pan, I. C., Deboer, C. J. & Hess, W. R. (1972). African swine fever: application of immuno electro osmophoresis for the detection of antibody. Canadian Journal of Comparative Medicine 36, 309–16.Google Scholar
Pan, I. C., Trautman, R., Hess, W. R., Deboer, C. J., Tessler, J., Ordas, A., Sanchez Botija, C., Ovejero, J. & Sanchez, M. C. (1974). African swine fever: comparison of four serotests on porcine serum in Spain. American Journal of Veterinary Research 35, 787–90.Google Scholar
Rosenthal, J. D., Hayashi, K. & Watkins, A. L. (1973). Comparison of direct and indirect solid phase microradioimmunoassays for the detection of viral antigens and antiviral antibody. Applied Microbiology 25, 567–73.Google Scholar
Williams, C. A. & Chase, M. W. (1967). Methods in Immunology and Immunochemistry, Vol. 1, p. 391. New York and London: Academic Press.Google Scholar