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Serological response of chickens to Salmonella enteritidis infection

Published online by Cambridge University Press:  15 May 2009

H. Chart ,
B. Rowe ,
A. Baskerville  and
T. J Humphrey
H. Chart
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, Colindale, London NW9 5HT, UK
B. Rowe
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, Colindale, London NW9 5HT, UK
A. Baskerville
Affiliation:
Division of Pathology, Centre for Applied Medical Research, Porton Down, Salisbury, Wiltshire SP4 OJG, UK
T. J Humphrey
Affiliation:
Public Health Laboratory, Church Lane, Heavitree, Exeter, Devon EX2 5AD, UK
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Summary

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Fifty-eight sera, from 29 chickens originating from two layer flocks known to be naturally infected with Salmonella enteritidis phage type (PT) 4, were examined for antibodies to S. enteritidis. Using the techniques of immunoblotting and ELISA, antibodies to the lipopolysaccharide (LPS) of S. enteritidis were detected in 43 of 58 sera. Antibodies were of the IgG class and bound to the S. enteritidis LPS antigen 0=12. Bacterial agglutination reactions using whole-cell preparations of S. enteritidis and S. pullorum, correlated with anti-LPS antibody reactions as detected by immunoblotting and ELISA. A rapid means of screening chicken sera for antibodies to the LPS of S. enteritidis as an indicator of infection is discussed.

Type
Research Article
Information
Epidemiology & Infection , Volume 104 , Issue 1 , February 1990 , pp. 63 - 71
Copyright
Copyright © Cambridge University Press 1990

References

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