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The sequential appearance of antibody and immunoglobins in nasal secretion after immunization of volunteers with live and inactivated influenza B virus vaccines

Published online by Cambridge University Press:  15 May 2009

Jean C. Downie
Jean C. Downie
Affiliation:
The Virus Research Laboratory of the University of Sheffield, Lodge Moor Hospital, Sheffield*
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Summary

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The sequential development of the immune response in nasal washings was studied in 54 volunteers immunized with either attenuated or inactivated influenza B/Eng/13/65 virus vaccines.

Eleven of the 15 volunteers given the inactivated vaccine by deep subcutaneous inoculation showed no rise in nasal wash protein or immunoglobins due to the immunization procedure nor was specific neutralizing antibody detected in their nasal washings after immunization. Neutralizing antibody was detected in nasal washings of three volunteers in this group who also showed a 20-fold or greater increase in serum haemagglutinin-inhibiting antibody after immunization and in one volunteer who had antibody present in pre-trial nasal washings.

Eleven of 15 volunteers who were successfully infected by the live attenuated vaccine showed a characteristic rise in protein and IgA and IgG immunoglobin concentrations in nasal washings 5–14 days after the administration of the live virus vaccine. Neutralizing antibody was detected in the nasal washings of these 11 volunteers and appeared at the same time as or 1–2 days after the initial rise of protein and immunoglobin. Neutralizing antibody was also detected in the nasal washings of one other volunteer who did not show a rise in protein or immunoglobin concentration in nasal washings after immunization.

IgA was detected (⋟ 3 mg./lOO ml.) in the majority (84%) of nasal wash specimens which had a protein concentration of 0·2 mg./ml. or greater while IgG was not detected (⋟ 4·5 mg./lOO ml.) until the protein concentration rose to 0·4 mg./ml. or greater. The geometric mean concentration for normal nasal wash protein in this study was 0·3 + 0·1 mg./ml.

Regression analysis indicated that the concentrations of both IgA and IgG immunoglobins were directly proportional to the protein concentration in nasal washings but that this relationship varied considerably between individuals.

Absorption studies indicated that neutralizing and haemagglutinin-inhibiting antibodies in nasal secretion to influenza B/Eng/13/65 virus were predominantly associated with the IgA class of immunoglobin.

Type
Research Article
Information
Journal of Hygiene , Volume 71 , Issue 3 , September 1973 , pp. 433 - 445
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

Alford, R. H., Rossen, R. D., Butler, W. T. & Kasel, J. A. (1967). Neutralizing and hemagglutination-inhibiting activity of nasal secretions following experimental human infection with A2 influenza virus. Journal of Immunology 98, 724.CrossRefGoogle ScholarPubMed
Artenstein, M. S., Bellanti, J. A. & Buescher, E. L. (1964). Identification of the antiviral substances in nasal secretion. Proceedings of the Society for Experimental Biology and Medicine 117, 558.CrossRefGoogle Scholar
Beare, A. S., Bynoe, M. L. & Tyrrell, D. A. J. (1968). Investigation into the attenuation of influenza viruses by serial passage. British Medical Journal iv, 482.CrossRefGoogle Scholar
Bellanti, J. A., Artenstein, M. S. & Buescher, E. L. (1965). Characterization of virus neutralizing antibodies in human serum and nasal secretions. Journal of Immunology 94, 344.CrossRefGoogle ScholarPubMed
Burnet, F. M., Lush, D. & Jackson, A. V. (1939). A virus inactivating agent from human nasal secretion. British Journal of Experimental Pathology 20, 377.Google Scholar
Butler, W. T., Rossen, R. D. & Waldmann, T. A. (1967). The mechanism of appearance of immunoglobulin A in nasal secretions in man. Journal of Clinical Investigation 46, 1883.CrossRefGoogle ScholarPubMed
Butler, W. T., Waldmann, T. A., Rossen, R. D., Douglas, R. G. Jr. & Couch, R. B. (1970). Changes in IgA and IgG concentrations in nasal secretions prior to the appearance of antibody during viral respiratory infection in man. Journal of Immunology 105, 584.CrossRefGoogle Scholar
Dixon, W. J. & Massey, F. J. (1957). Introduction to Statistical Analysis, 2nd ed.New York: McGraw-Hill.CrossRefGoogle Scholar
Downie, J. C. (1970). Neuraminidase and hemagglutinin-inhibiting antibodies in serum and nasal secretions of volunteers immunized with attenuated and inactivated influenza B/Eng/13/65 virus vaccines. Journal of Immunology 105, 620.CrossRefGoogle ScholarPubMed
Downie, J. C. & Stuart-Harris, C. H. (1970). The production of neutralizing activity in serum and nasal secretion following immunization with influenza B virus. Journal of Hygiene 68, 233.CrossRefGoogle ScholarPubMed
Fazekas De St Groth, S. & Donelley, M. (1950). Studies in experimental immunology of influenza. IV. The protective value of active immunization. Australian Journal of Experimental Biology and Medical Science 28, 61.CrossRefGoogle ScholarPubMed
Fazekas de St Groth, S. & Webster, R. G. (1966). Disquisitions on original antigenic sin. I. Evidence in man. Journal of Experimental Medicine 124, 331.CrossRefGoogle Scholar
Finter, N. B. (1967). Quantitative hemadsorption, a new assay technique. II. Assay of neutralizing antibodies to hemadsorbing viruses. Journal of Immunology 98, 88.CrossRefGoogle ScholarPubMed
Fluk, R. V., Fedson, D. S., Huber, M. A., Fitzpatrick, J. R. & Kasel, J. A. (1970). Antibody responses in serum and nasal secretions according to age of recipient and method of administration of A2/Hong Kong/68 inactivated influenza virus vaccine. Journal of Immunology 104, 8.CrossRefGoogle Scholar
Francis, T. Jr. (1941). The significance of nasal factors in epidemic influenza. Problems and Trends in Virus Research, pp. 41–54. University of Pennsylvania Press.Google Scholar
Kasel, J. A., Hume, E. B., Fluk, R. V., Togo, Y., Huber, M. A. & Hornick, R. B. (1969). Antibody responses in nasal secretions and serum of elderly persons following local or parenteral administration of inactivated influenza virus vaccine. Journal of Immunology 102, 555.Google ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with Folin phenol reagent. Journal of Biological Chemistry 193, 265.CrossRefGoogle ScholarPubMed
Mancini, C., Carbonara, A. O. & Heremans, J. F. (1965). Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2, 235.CrossRefGoogle ScholarPubMed
Mann, J. J., Waldman, R. H., Yogo, Y., Heiner, G. G., Dawkins, A. T. & Kasel, J. A. (1968). Antibody response in respiratory secretions of volunteers given live and dead influenza virus. Journal of Immunology 100, 726.CrossRefGoogle ScholarPubMed
Mostow, S. E., Sohoenbaum, S. C., Dowdlb, W. R., Coleman, M. T., Kaye, H. S., & Hierholzer, J. C. (1970). Studies on inactivated influenza vaccines. II. Effect of increasing dosage on antibody response and adverse reactions in man. American Journal of Epidemiology 92, 248.Google ScholarPubMed
Remington, J. S., Vosti, K. L., Lietze, A. & Zimmerman, A. L. (1964). Serum proteins and antibody activity in human nasal secretions. Journal of Clinical Investigation 43, 1613.CrossRefGoogle ScholarPubMed
Rossen, R. D., Butler, W. T., Cate, T. R., Szwed, C. F. & Couch, R. B. (1965). Protein composition of nasal secretion during respiratory virus infection. Proceedings of the Society for Experimental Biology and Medicine 119, 1169.CrossRefGoogle ScholarPubMed
Rossen, R. D., Butler, W. T., Waldman, R. H., Alford, R. H., Hornick, R. B., Togo, Y. & Kasel, J. A. (1970). The proteins in nasal secretion. II. A longitudinal study of IgA and neutralizing antibody levels in nasal washings from men infected with influenza virus. Journal of the American Medical Association 211, 1157.CrossRefGoogle Scholar
Rossen, R. D., Morgan, C., Hsu, K. C., Butler, W. T. & Rose, H. M. (1968). Localization of 11S external secretory IgA by immunofluorescence in tissues lining the oral and respiratory passages in man. Journal of Immunology 100, 706.CrossRefGoogle Scholar
Rossen, R. D., Schade, A. L., Butler, W. T. & Kasel, J. A. (1966). The proteins in nasal secretion: a longitudinal study of the γ-A globulin, γ-G globulin, albumin, siderophilin, and total protein concentrations in nasal washings from adult volunteers. Journal of Clinical Investigation 45, 768.CrossRefGoogle Scholar
Smith, C. B., Purcell, R. H., Bellanti, J. A. & Chanock, R. M. (1966). Protective effect of antibody to parainfluenza type 1 virus. New England Journal of Medicine 275, 1145.CrossRefGoogle ScholarPubMed
South, M. A., Cooper, M. D., Wollheim, F. A., Hong, R. & Good, R. A. (1966). The IgA System. I. Studies of the transport and immunochemistry of IgA in the saliva. Journal of Experimental Medicine 123, 615.CrossRefGoogle Scholar
Tomasi, T. B., Tan, E. M., Solomon, A. & Prendergast, R. A. (1965). Characteristics of an immune system common to certain external secretions. Journal of Experimental Medicine 121, 101.CrossRefGoogle ScholarPubMed
Waldman, R. H., Mann, J. J. & Kasel, J. A. (1968). Influenza virus neutralizing antibody in human respiratory secretions. Journal of Immunology 100, 80.CrossRefGoogle ScholarPubMed