Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-12-02T19:24:14.004Z Has data issue: false hasContentIssue false
  • English
  • Français

Sampling methods for monitoring changes in gonococcal populations

Published online by Cambridge University Press:  15 May 2009

K. M. Bindayna  and
C. A. Ison
K. M. Bindayna
Affiliation:
Department of Medical Microbiology, St Mary's Hospital Medical School, Paddington, London W2 1PG
C. A. Ison*
Affiliation:
Department of Medical Microbiology, St Mary's Hospital Medical School, Paddington, London W2 1PG
*
*Author for correspondence.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A total of 160 consecutive isolates of Neisseria gonorrhoeae was collected over a 3-month period. They were tested for their susceptibility to penicillin, erythromycin and spectinomycin and the auxotype and the serotype determined. We have evaluated two sampling methods, the collection of every fifth isolate and the first 20 isolates (10 male and 10 female) each month, to determine whether either is representative of the total population. There was no significant difference between either method of sampling and the total for detecting the predominant auxotypes and serovars or the distributions in antibiotic susceptibility. It is possible to monitor major changes in a gonococcal population, particularly susceptibility to antibiotics, using a sample of the total population.

Type
Special Article
Information
Epidemiology & Infection , Volume 103 , Issue 1 , August 1989 , pp. 203 - 209
Copyright
Copyright © Cambridge University Press 1989

References

REFERENCES

1.Cannon, JG, Sparling, PF. The genetics of the gonococcus. Ann Rev Microbiol 1984; 38: 111–23.CrossRefGoogle ScholarPubMed
2.Ashford, WA, Golash, RG, Hemming, VG. Penicillinase-producing Neisseria gonorrhoeae. Lancet 1976; 2: 657–8.CrossRefGoogle ScholarPubMed
3.Phillips, I.β-lactamase producing penicillin-resistant gonococcus. Lancet 1976; 2: 656–7.CrossRefGoogle ScholarPubMed
4.Brown, S, Warnnissorn, T, Biddle, J, Panikabutra, K, Traisupa, A.Antimicrobial resistance of Neisseria gonorrhoeae in Bangkok: Is single drug treatment passé? Lancet 1982; 2: 1366–8.CrossRefGoogle ScholarPubMed
5.Faruki, PH., Kohmescher, RN, McKinney, P, Sparling, PF. A community-based outbreak of infection with penicillin-resistant Neisseria gonorrhoeae not producing penicillinase (chromosomally mediated resistance). New Engl J Med 1985; 313: 607–11.CrossRefGoogle Scholar
6.Rice, RJ, Biddle, JW, JeanLouis, YA, DeWitt, WE, Blount, JH, Morse, SA. Chromsomally mediated resistance in Neisseria gonorrhoeae in the United States: results of surveillance and reporting, 1983–1984. J Infect Dis 1986; 153: 340–5.CrossRefGoogle Scholar
7.Dowsett, EG. Penicillin-resistant gonococci. Lancet 1980; 2: 202.Google ScholarPubMed
8.Ison, CA, Gedney, J, Easmon, CSF. Chromosomal resistance of gonococci to antibiotics. Genitourin Med 1987; 63: 239–43.Google ScholarPubMed
9.Catlin, BW. Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing. J Infect Dis 1973; 128: 178–94.CrossRefGoogle ScholarPubMed
10.Knapp, JS, Holmes, KK. Disseminated gonococcal infection caused by Neisseria gonorrhoeae with unique nutritional requirements. J Infect Dis 1975; 132: 204–8.CrossRefGoogle ScholarPubMed
11.Wang, SP, Holmes, KK, Knapp, JS, Ott, S, Kyzer, D.Immunologic classification of Neisseria gonorrhoeae with microimmunofluorescence. J Immunol 1977; 119: 795–803.CrossRefGoogle ScholarPubMed
12.Buchanan, TM, Hildebrandt, JF. Antigen-specific serotyping of Neisseria gonorrhoeae: characterisation based upon principal outer membrane protein. Infect Immun 1981; 32: 985–94.CrossRefGoogle ScholarPubMed
13.Sandstrom, EG, Danielsson, D.Serology of Neisseria gonorrhoeae. Classification by coagglutination. Acta Path Micro Scand Sect B 1980; 88: 27–38.Google ScholarPubMed
14.Knapp, JS, Tam, MR, Nowinski, RC, Holmes, KK, Sandstrom, EG. Serological classification of Neisseria gonorrhoeae with use of monoclonal antibodies to gonococcal outer membrane protein I. J Infect Dis 1984; 150: 44–8.CrossRefGoogle ScholarPubMed
15.Knapp, JS, Holmes, KK, Bonin, P, Hook, EW. IIIEpidemiology of gonorrhoea: distribution and temporal changes in auxotype/serovar classes of Neisseria gonorrhoeae. Sex Transm Dis 1987; 14: 26–32.CrossRefGoogle ScholarPubMed
16.Copley, CG, Egglestone, SI. Auxotyping of Neisseria gonorrhoeae isolated in the United Kingdom. J Med Microbiol 1983; 16: 295–302.CrossRefGoogle ScholarPubMed
17.Tam, MR, Buchanan, TM, Sandstrom, EG, Holmes, KK, Knapp, JS, Siadak, AW, Nowinski, RC. Serological classification of Neisseria gonorrhoeae with monoclonal antibodies. Infect Immun 1982; 36: 1042–53.CrossRefGoogle ScholarPubMed
18.Rice, RJ, Hook, EW III, Holmes, KK, Knapp, JS Evaluation of sampling methods for surveillance of Neisseria gonorrhoeae strain populations. In: Poolman, JT et al. , ed. Gonococci and meningococci. Dordrecht: Kluwer Academic Publishers, 167–73.Google Scholar
19.Woodford, N, Bindayna, KM, Easmon, CSF, Ison, CA.Associations between serotype and susceptibility to antibiotics of Neisseria gonorrhoeae. Genitourin Med 1989. In press.Google ScholarPubMed