Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-30T21:36:56.176Z Has data issue: false hasContentIssue false
  • English
  • Français

Rubella serology: a comparison of four methods for exclusion of non-specific serum inhibitors

Published online by Cambridge University Press:  25 March 2010

T. Traavik ,
O. Spanne  and
S. Mennen
T. Traavik
Affiliation:
Institute of Medical Biology, University of Tromsø, N-9001 Tromsø, Norway
O. Spanne
Affiliation:
Institute of Medical Biology, University of Tromsø, N-9001 Tromsø, Norway
S. Mennen
Affiliation:
Department of Microbiology, Regional Hospital and University Clinic, Tromsø
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The ability of the pyrogenic silica Aerosil 380R to exclude non-specific serum inhibitors (NSI) of rubella virus haemagglutination was evaluated. The developed procedure was compared with the kaolin, heparin/MnCl2 and dextran sulphate/CaCl2 methods.

Aerosil and kaolin were found superior for the elimination of non-specific inhibitors and high density lipoproteins (HDL). The other methods left NSI and HDL in a majority of the sera, occasionally in high titres. Aerosil seemed to be somewhat more efficient than kaolin in NSI and HDL exclusion. The Aerosil method offers the opportunity to detect sera with rubella antibody titres < 10. Among eight such sera, six were shown to contain rubella antibodies, while two were false positives.

Type
Research Article
Information
Journal of Hygiene , Volume 86 , Issue 3 , June 1981 , pp. 315 - 327
Copyright
Copyright © Cambridge University Press 1981

References

REFERENCES

Allen, R. & Hedlund, K. (1976). Simple procedure for the removal of nonspecific inhibitors of rubella virus hemagglutination. Journal Clinical Microbiology 2, 524–7.CrossRefGoogle Scholar
Beaumont, J. L., Carlson, L. A., Cooper, G. R., Fejfar, Z., Fredrickson, D. S. & Strasser, T. (1970). Classification of hyperlipidaemias and hyperlipidproteinaemias. Bulletin World Health Organization 43, 891–915.Google Scholar
Bernfeld, P., Nisselbaum, J. S., Berkeley, B. J. & Hanson, R. W. (1960). The influence of chemical and physiochemical nature of macromolecular polyanions on their interaction with human serum-lipoproteins. Journal Biological Chemistry. 235, 2852–9.CrossRefGoogle Scholar
Biano, S. A., Chang, T. W. & Daniels, J. B. (1974). Rubella hemagglutination inhibition: removal of non-specific agglutination due to manganous chloride. Applied Microbiology 28, 992–4.CrossRefGoogle Scholar
Blom, H. & Haukenes, G. (1974). Identification of non-specific serum inhibitors of rubella virus hemagglutination. Medical Microbiology and Immunology 159, 271–7.CrossRefGoogle Scholar
Chang, T. W. & Weinstein, L. (1972). Studies of non-specific inhibition of the hemagglutinin of rubella virus and isolation and identification of the inhibitor. American Journal of the Medical Sciences 263, 233–9.CrossRefGoogle Scholar
Cooper, L. Z., Matters, B., Rosenblum, J. K. & Krugman, S. (1969). Experience with a modified rubella hemagglutination antibody test. JAMA 207, 89–93.CrossRefGoogle Scholar
Ellis, M. L. & Campbell, J. B. (1977). Use of sodium polyanetholesulfate-CaCl2 for removal of serum non-specific inhibitors of rubella hemagglutination: Comparison with other polyanion-divalent cation combinations. Journal of Clinical Microbiology 6, 348–58.CrossRefGoogle Scholar
Feldman, H. A. (1968). Removal by heparin-MnCl2, of non-specific rubella hemagglutinin serum inhibitor. Proceedings of the Society of Experimental Biology 127, 570–3.CrossRefGoogle Scholar
Halonen, P. E., Ryan, J. M. & Stewart, J. A. (1967). Rubella hemagglutinin prepared with alkaline extraction of virus grown in suspension culture of BHK 21 cells. Proceedings of the Society of Experimental Biology 125, 162–7.CrossRefGoogle ScholarPubMed
Haukenes, G. (1973). A rubella haemagglutination inhibitor simulating antibody. Acta pathologica et microbiologica scandinavica Section B 81, 719–23.CrossRefGoogle ScholarPubMed
Haukenes, G. & Blom, H. (1975). False positive rubella virus haemagglutination inhibition reactions: Occurrence and disclosure. Medical Microbiology and Immunology 161, 99–106.CrossRefGoogle ScholarPubMed
Ho, W. K. K. & Shortridge, K. F. (1977). Serum lipids as inhibitors of hemagglutination by rubella virus. Lipids 12, 85–91.CrossRefGoogle ScholarPubMed
Iwasa, S. & Hori, M. (1976). Improved rubella hemagglutination test: inactivation of non-immunoglobulin hemagglutination inhibitors by phospholipase C. Journal of Clinical Microbiology 4, 461–6.CrossRefGoogle ScholarPubMed
Kalimo, K. O. K., Meurman, O. H., Halonen, P. E., Ziola, B. R., Viljanen, M. K., Granfors, K. & Toivanen, P. (1976). Solid-phase radioimmunoassay of rubella virus IgG and IgM antibodies. Journal of Clinical Microbiology 4, 117–23.CrossRefGoogle Scholar
Liebhaber, H. (1970). Measurement of rubella antibody by hemagglutination inhibition. II. Characteristics of an improved HAI test employing a new method for the removal of non-immunoglobulin HA inhibitors from serum. Journal of Immunology 104, 826–34.CrossRefGoogle ScholarPubMed
Nelson, D. B., Quiring, E. P. & Inhorn, S. L. (1972). Improved dextran sulfate-calcium chloride method for the removal of non-specific inhibitors with modifications for non-specific agglutinin removal in the rubella hemagglutination inhibition test. Applied Microbiology 27, 767–70.CrossRefGoogle Scholar
Schmidt, N. J. & Lennette, E. H. (1970). Variables in the rubella hemagglutination-inhibition test system and their effect on antigen and antibody titers. Applied Microbiology 19, 491–504.CrossRefGoogle ScholarPubMed
Schneweis, K. E., Wolff, M. H. & Marklein, G. (1972), Sindbis-Virus zur Kontrolle der vollstandigen Beseitigung unspezifischer Inhibitoren beim Røteln-Hemagglutinasjons-test und deren Eliminierung. Zeistschrift für Immunitäts Forschung 144, 75–87.Google Scholar
Shortridge, K. F., Biddle, F. & Pepper, D. S. (1972). Rubella virus non-specific hemagglutination inhibitor: evidence for the role of glycolipid bound to low density (LDL) lipoprotein. Clinica chimica acta 42, 285–94.CrossRefGoogle Scholar
Shortridge, K. F. & Ho, W. K. K. (1974). Human serum lipoproteins as inhibitors of hemagglutination for selected togaviruses. Journal of General Virology 23, 113–16.CrossRefGoogle ScholarPubMed
Shortridge, K. F. & Ho, W. K. K. (1976). Comparison of the activities of hemagglutination by different togaviruses for human serum lipoproteins and their constituents. Journal of General Virology 33, 523–7.CrossRefGoogle ScholarPubMed
Siebke, J. C., Kjeldsberg, E. & Traavik, T. (1972). On the adsorption of Australia antigen to a colloidal silica and some possible applications. Acta pathologica et microbiologica scandinavica Section B 80, 935–6.Google ScholarPubMed
Skaug, K.Ørstavik, I. & Ulstrup, J. C. (1975). Application of the passive haemolysis test for the determination of rubella virus antibodies. Acta Pathologica microbiologica et scandinavica Section B 83, 367–72.CrossRefGoogle ScholarPubMed
Steinmann, J. (1977 a). Lipoproteins as serum inhibitors in rubella hemagglutination inhibition test and their elimination. Zbl. Bakt. Hyg. I. Abt. Orig. A, 239, 10–17.Google ScholarPubMed
Steinmann, J. (1977 b). Serum-treatment in the rubella HI-test by means of silicic acid. Zbl. Bakt. Hyg., I. Abt. Orig. A. 239, 157–61.Google ScholarPubMed
Stephan, W. (1971). Hepatitis free and stable human serum for intravenous therapy. Vox. Sanguinis 20, 442–57.CrossRefGoogle ScholarPubMed
Strannegård, O., Grillner, L. & Lindberg, I. M. (1975). Haemolysis-in-gel test for the demonstration of antibodies to rubella virus. Journal of Clinical Microbiology 1, 491–4.CrossRefGoogle Scholar
Traavik, T. (1975). A method for production of antisera to hepatitis B antigen subtypes, and the distribution of subtypes d and y among Norwegian hepatitis B patients. Acta Pathologica microbiologica et scandinavica Section B 83, 153–6.CrossRefGoogle ScholarPubMed
Traavik, T. (1977). Improvement of arbovirus HA antigens by treatment with a colloidal silica gel and sonication. Archives of Virology 54, 223–9.CrossRefGoogle ScholarPubMed
Traavik, T. (1978). Runde virus: Further characteristics and a method for purification. Acta pathologica microbiologica et scandinavica Section B. 86, 349–53.CrossRefGoogle Scholar
Traavik, T., Mehl, R. & Wiger, R. (1978). California encephalitis group viruses isolated from mosquitoes collected in Southern and Arctic Norway. Acta pathologica microbiologica et scandinavica Section B 86, 335–41.CrossRefGoogle Scholar
Traavik, T., Siebke, J. C. & Kjeldsberg, E. (1972). A sensitive modification of the Ouchterlony technique detection of hepatitis-associated antigen in a closed hexagonal system. Acta pathologica microbiologica et scandinavica Section B 80, 773–4.CrossRefGoogle Scholar
U.S. Public Health Service (1975). CDC standard rubella hemagglutination-inhibition test. Center for Disease Control, Washington D.C.Google Scholar
Voller, A. & Bidwell, D. E. (1975). A simple method for detecting antibodies to rubella British Journal of Experimental Pathology 56, 338–9.Google ScholarPubMed