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Restricted variability of a 17 nucleotide stretch within the 5′-noncoding region of poliovirus genome

Published online by Cambridge University Press:  15 May 2009

T. Pöyry ,
L. Kinnunen  and
T. Hovi
T. Pöyry
Affiliation:
Enterovirus Laboratory, Department of Virology, and Molecular Biology Unit, Virology Group, National Public Health Institute, Helsinki, Finland
L. Kinnunen
Affiliation:
Enterovirus Laboratory, Department of Virology, and Molecular Biology Unit, Virology Group, National Public Health Institute, Helsinki, Finland
T. Hovi
Affiliation:
Enterovirus Laboratory, Department of Virology, and Molecular Biology Unit, Virology Group, National Public Health Institute, Helsinki, Finland
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Summary

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The outbreak of poliomyelitis in Finland in 1984 was caused by a wild strain of poliovirus 3 with uncommon molecular and antigenic properties. We prepared a synthetic oligonucleotide probe complementary to nucleotides 494–510 in the 5′-noncoding part of the genome of a representative strain of the outbreak. This short nucleotide stretch was found to be relatively well conserved within the outbreak and uncommon among 82 independent poliovirus isolates. It may thus be a useful marker for screening isolates to identify those requiring more detailed genetic comparison. The sequences of the corresponding region of the genome are known for 32 separate poliovirus strains and 3 coxsackie B virus strains and show 6 fully conserved nucleotides that could assume a constant hairpin-loop position in a hypothetical secondary structure of the RNA. This could explain the persistence of a particular 17 nucleotide sequence for 40 years in nature in this highly variable region of the poliovirus genome.

Type
Research Article
Information
Epidemiology & Infection , Volume 103 , Issue 3 , December 1989 , pp. 671 - 683
Copyright
Copyright © Cambridge University Press 1989

References

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