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Replicon typing characterization of plasmids encoding resistance to gentamicin and apramycin in Escherichia coli and Salmonella typhimurium isolated from human and animal sources in Belgium

Published online by Cambridge University Press:  15 May 2009

P. Pohl ,
Y. Glupczynski ,
M. Marin ,
G. Van Robaeys ,
P. Lintermans  and
M. Couturier
P. Pohl
Affiliation:
Institut National de Recherches Vétérinaires, Groeselenberg 99, 1180 Brussels Belgium
Y. Glupczynski
Affiliation:
Service de Microbiologie, Hôpital Universitaire Brugmann, 4 Place A. van Gehuchten, 1020 Brussels, Belgium
M. Marin
Affiliation:
Institut National de Recherches Vétérinaires, Groeselenberg 99, 1180 Brussels Belgium
G. Van Robaeys
Affiliation:
Institut National de Recherches Vétérinaires, Groeselenberg 99, 1180 Brussels Belgium
P. Lintermans
Affiliation:
Institut National de Recherches Vétérinaires, Groeselenberg 99, 1180 Brussels Belgium
M. Couturier
Affiliation:
Service de Génétique, Université Libre de Bruxelles, 67 rue des Chevaux, 1640 Rhode-St-Genèse, Belgium.
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Escherichia coli and salmonella strains with plasmids conferring resistance to gentamicin and apramycin have been isolated with increasing frequency both from cattle and hospital patients in Belgium. The apramycin-gentamicin resistance plasmids were characterized in recipient strains by their profiles and molecular weights using agarose gel electrophoresis, by their antimicrobial resistance patterns and by replicon typing using a series of DNA probes specific for the genes controlling their systems of replication. Overall, most of the plasmids differed in their DNA electrophoretic patterns. Seventeen different antimicrobial resistance profiles were observed, and there were six different types of replicons. However, two replication genes predominated and had a preferential distribution in different bacterial species. The rep FIC.a plus rep Q multireplicon was found mainly in plasmids recovered from gentamicin- and apramycin-resistant E. coli while replicon of the type rep FIC.b largely prevailed in S. typhimurium. Identical replication genes were found in most animal and human strains, hence suggesting a high homology between apramycin- gentamicin plasmids in these communities. Finally, our results indicate that the rapid spread of apramycin-gentamicin-resistance in several species of Enterobacteriaceae isolated from animals and from humans in Belgium is not due to a single plasmid, but rather that the gene encoding AAC(3)-IV is carried by various replicons.

Type
Research Article
Information
Epidemiology & Infection , Volume 111 , Issue 2 , October 1993 , pp. 229 - 238
Copyright
Copyright © Cambridge University Press 1993

References

REFERENCES

1.Sojka, WJ, Wray, C, Pritchard, GC, Hedges, RW. Gentamicin resistant salmonella. Vet Rec 1982; 111: 512.Google Scholar
2.Sojka, WJ, Wray, C, McLaren, I. Survey of drug resistance in Salmonellae isolated from animals in England and Wales from 1979 to 1981. Brit Vet J 1984; 140: 576–91.CrossRefGoogle ScholarPubMed
3.Chaslus-Danela, E, Lafont, J-P. Resistance to gentamicin and apramycin in Escherichia coli from calves in France. Vet Rec 1985; 177: 90–1.CrossRefGoogle Scholar
4.Chaslus-Danela, E, Martel, J, Carlier, C, Lafont, J-P, Courvalin, P. Emergence of aminoglycoside 3-N-acetyltransferase IV in Escherichia coli and Salmonella typhimurium isolated from animals in France. Antimicrob Agents Chemother 1986; 29: 239–43.CrossRefGoogle Scholar
5.Chaslus-Dancla, E, Glupczynski, Y, Gerbaud, G, Lagorce, M, Lafont, J-P, Courvalin, P. Detection of apramycin resistant Enterobacteriaceae in hospital isolates. FEMS Microbiol Lett 1989; 61: 261–5.CrossRefGoogle Scholar
6.Wray, C, Beedell, YE, McLaren, IM. A survey of antimicrobial resistance in Salmonellae isolated from animals in England and Wales during 1984–1987. Br Vet J 1991; 147: 356–69.CrossRefGoogle ScholarPubMed
7.Davies, J, O'Connor, S. Enzymatic modification of aminoglycoside antibiotics: 3-N-acetyltransferase with broad specificity that determines resistance to the novel aminoglycoside apramycin. Antimicrob Agents Chemother 1978; 14: 69–72.CrossRefGoogle Scholar
8.Pohl, P, Verlinden, M, Lintermans, P, Van Robaeys, G, Stockmans, F. Antibiogrammes des entérobactéries pathogènes pour les animaux d'élevage et les pigeons, isolées en Belgique de 1986 à 1990. Ann Méd Vét 1991; 135: 101–8.Google Scholar
9.Glupczynski, Y, Dewit, J, Hare, RS, Miller, HG, Yourassowsky, E. Emergence of aminoglycoside 3-N-Acetyltransferase type IV in Escherichia coli isolated from patients in Belgium. Abstr Ann Meet Am Soc Microbiol 1988; A– 82: 14.Google Scholar
10.Gomez-Lus, R, Rivera, MJ, Gomez-Lus, ML, et al. Resistance to apramycin in two enterobacterial clinical isolates: detection of a 3-N-acetyltransferase IV. J Chemother 1990; 12: 241–3.CrossRefGoogle Scholar
11.Gomez-Lus, R, Gil, J, Gomez-Lus, ML, Castillo, J, Bouza, E, Rubio, MC. Resistance to apramycin in Klebsiella pneumoniae isolated from patients: detection of 3-N-acetyl-transferase IV. 27th Intersc Conf Antimicrob Agents Chemother 1987; abstr. no. 400.Google Scholar
12.Braü, B, Pilz, U, Piepersberg, W. Genes for gentamicin-(3)-N-acetyltransferases III and IV: I-nucleotide sequence of the AAC(3)-IV gene and possible involvement of an ISI40 element in its expression. Mol Gen Genet 1984; 193: 179–87.CrossRefGoogle Scholar
13.Chaslus-Dancla, E, Pohl, P, Meurisse, M, Marin, M, Lafont, J-P. High genetic homology between plasmids of human and animal origins conferring resistance to the aminoglycosides gentamicin and apramycin. Antimicrob Agents Chemother 1991; 35: 590–3.CrossRefGoogle Scholar
14.Couturier, M, Bex, F, Bergquist, PL, Maas, WK. Identification and classification of bacterial plasmids. Microbiol Rev 1988; 52: 375–95.CrossRefGoogle ScholarPubMed
15.Pohl, P, Thomas, J. Nouveau milieu d'isolement et de détection des Salmonella. Ann Inst Pasteur de Lille 1966; 126A: 435–48.Google Scholar
16.Ewing, WH. Differentiation of Enterobacteriaceae by biochemical reactions. In: Ewing, WH, ed. Identification of Enterobacteriaceae, 4th ed. New York: Elsevier, 1986. 47–72.Google Scholar
17.National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial disk susceptibility tests. Approved standard 1990; Vol. 10, nr. 7. National Committee for Clinical Laboratory Standards, Villanova, Pennsylvania.Google Scholar
18.Shimizu, K, Kumada, T, Hsieh, WC et al. Comparison of aminoglycoside resistance patterns in Japan, Formosa, and Korea, Chile, and the United States. Antimicrob Agents Chemother 1985; 28: 282–8.CrossRefGoogle ScholarPubMed
19.Lesage, DD, Gerbaud, GB, Chabbert, YA. Carte génétique et structure chez Escherichia coli K12 d'un plasmide de résistance isolé de Salmonella ordonez. Ann Inst Pasteur Microbiol 1975; 126A: 435–48.Google ScholarPubMed
20.Anderson, ES, Lewis, MJ. Characterization of a transfer factor associated with drug resistance in Salmonella typhimurium. Nature 1965; 208: 843–9.CrossRefGoogle ScholarPubMed
21.Popoff, MY, Myras, I, Coynault, C, Lasselin, C, Pardon, P. Molecular relationship between virulence plasmids of Salmonella serotypes typhimurium and dublin and large plasmids of other Salmonella serotypes. Ann Inst Pasteur Microbiol 1984; 135A: 389–98.CrossRefGoogle ScholarPubMed
22.Portnoy, DA, Moseley, SL, Falkow, S. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun 1981; 31: 775–82.CrossRefGoogle ScholarPubMed
23.Macrina, FL, Kopecko, DJ, Jones, KR, Ayers, DH, McGowen, SM. A multiple plasmid containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid 1978; 1: 417–20.CrossRefGoogle ScholarPubMed
24.Thiry, G. Plasmids of the epiphytic bacterium Erwinia uredovora. J Gen Microbiol 1984: 130, 1623–31.Google Scholar
25.Salauze, D, Otal, I, Gomez-Lus, R, Davies, J. Aminoglycoside acetyltransferase 3–IV (aacC4) and hygromycin B 4-I phosphotransferase (hphB) in bacteria isolated from human and animal sources. Antimicrob Agents Chemother 1990; 34: 1915–20.CrossRefGoogle ScholarPubMed
26.Hedges, RW, Rodriguez-Lemoine, V, Datta, N. R-factors from Serratia marcescens. J Gen Microbiol 1975; 86: 88–92.CrossRefGoogle ScholarPubMed