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Reducing the endotoxic activity of pertussis vaccine

Published online by Cambridge University Press:  25 March 2010

R. M. Bannatyne  and
Rose Cheung
R. M. Bannatyne
Affiliation:
Department of Bacteriology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8
Rose Cheung
Affiliation:
Department of Bacteriology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8
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Summary

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Unadsorbed, regular production pertussis vaccine was treated with polymyxin B sulphate at concentrations of 25,50 and 100 μg/ml. The toxic activity of treated and untreated vaccines was compared using both the limulus amoebocyte lysate test and the mouse-weight-gain test. Protective efficacy was also assessed by the mouse protection test. No discernible effect on either toxicity or efficacy of the pertussis vaccine was observed. When the vaccine was treated with 5000 μg/ml of polymyxin, endotoxic activity assessed by the limulus lysate test appeared to be abolished.

Type
Research Article
Information
Journal of Hygiene , Volume 87 , Issue 3 , December 1981 , pp. 377 - 381
Copyright
Copyright © Cambridge University Press 1981

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