Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-27T23:56:15.508Z Has data issue: false hasContentIssue false
  • English
  • Français

Preservation of Staphylococcus aureus with unstable antibiotic resistance by drying

Published online by Cambridge University Press:  15 May 2009

D. I. Annear  and
W. B. Grubb
D. I. Annear
Affiliation:
Department of Microbiology, Royal Perth Hospital, and Department of Microbiology, The University of Western Australia
W. B. Grubb
Affiliation:
Department of Microbiology, Royal Perth Hospital, and Department of Microbiology, The University of Western Australia
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Cultures of Staphylococcus aureus showing unstable resistance to kanamycin, streptomycin, lincomycin and penicillin have been preserved by drying. Particular attention has been paid to organisms showing linked resistance to pairs of these antibiotics. Recoveries of viable cells from the desiccates was high and the proportion of resistant and sensitive cells was maintained both during storage and during heating. The preservation of the organisms on ceramic beads stored in air over silica gel promises to be an effective and convenient method for maintaining staphylococcal desiccates during studies of unstable antibiotic resistance.

Type
Research Article
Information
Journal of Hygiene , Volume 71 , Issue 2 , June 1973 , pp. 411 - 416
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

Annear, D. I. (1962). Recoveries of bacteria after drying on cellulose fibres. Australian Journal of Experimental Biology and Medical Science 40, 1.CrossRefGoogle ScholarPubMed
Annear, D. I. (1964). Recoveries of bacteria after drying in glutamate and other substances. Australian Journal of Experimental Biology and Medical Science 42, 717.CrossRefGoogle ScholarPubMed
Annear, D. I. (1970). Recoveries of bacteria after drying and heating in glutamate foams. Journal of Hygiene 68, 457.CrossRefGoogle ScholarPubMed
Annear, D. I. (1971). Recoveries of Salmonella ndolo from desiccates exposed to 100° C. under various conditions with respect to desiccant, desiccant temperature and atmosphere. Journal of Hygiene 69, 141.CrossRefGoogle ScholarPubMed
Annear, D. I. & Grubb, W. B. (1972 a). Linked and unstable resistance to kanamycin and penicillin, and diffusible pigment production in an isolate of Staphylococcus aureus. Journal of Medical Microbiology 5, 109.CrossRefGoogle Scholar
Annear, D. I. & Grubb, W. B. (1972 b). Unstable resistance to kanamycin, lincomycin and penicillin in a methicillin resistant culture of Staphylococcus aureus. Pathology (in the Press).CrossRefGoogle Scholar
Grivell, A. R. & Jackson, J. F. (1969). Microbial culture preservation with silica gel. Journal of General Microbiology 58, 423.CrossRefGoogle ScholarPubMed
Grubb, W. B. & Annear, D. I. (1972). Linked extrachromosomal resistance to streptomycin and kanamycin in Staphylococcus aureus. Journal of Medical Microbiology (in the Press).CrossRefGoogle ScholarPubMed
Lange, B. J. & Boyd, W. J. R. (1968). Preservation of fungal spores by drying on porcelain beads. Phytopathology 58, 1711.Google Scholar
Polding, J. B. (1943). A simple method of preserving bacteria dried in vacuo. Journal of Pathology and Bacteriology 55, 502.CrossRefGoogle Scholar