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PCR-RFLP typing of ureC from Helicobacter pylori isolated in Argentina from gastric biopsies before and after treatment with clarithromycin

Published online by Cambridge University Press:  01 April 1997

G. G. STONE ,
D. SHORTRIDGE ,
R. K. FLAMM ,
J. BEYER ,
D. STAMLER  and
S. K. TANAKA
G. G. STONE
Affiliation:
Anti-Infective Research Division, Abbott Laboratories, Abbott Park, Illinois
D. SHORTRIDGE
Affiliation:
Anti-Infective Research Division, Abbott Laboratories, Abbott Park, Illinois
R. K. FLAMM
Affiliation:
Anti-Infective Research Division, Abbott Laboratories, Abbott Park, Illinois
J. BEYER
Affiliation:
Anti-Infective Research Division, Abbott Laboratories, Abbott Park, Illinois
D. STAMLER
Affiliation:
Abbott International Division, Abbott Laboratories, Abbott Park, Illinois
S. K. TANAKA
Affiliation:
Anti-Infective Research Division, Abbott Laboratories, Abbott Park, Illinois
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Abstract

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A clinical trial was conducted in Argentina to determine the efficacy of clarithromycin plus lansoprazole for the treatment of Helicobacter pylori in duodenal ulcers and non-ulcer dyspepsia. PCR–RFLP was conducted on an 820-bp amplified product of the ureC gene of H. pylori to determine the genetic heterogeneity of 83 pretreatment and 21 post-treatment isolates. Twelve different restriction patterns were observed when digested with Sau 3A or Hha I, resulting in 40 different RFLP types. Comparison of isolates before treatment to after treatment showed that 20 of 20 patients had the same RFLP type. In addition, the presence of the cytotoxin-associated gene (cagA) and the vacuolating gene (vacA) were determined. All pretreatment isolates were positive for vacA whereas 75% of the pretreatment isolates were positive for cagA. The results of this study indicate that a high degree of heterogeneity exists among H. pylori and that infection is not limited to a small number of RFLP types.

Type
Research Article
Information
Epidemiology & Infection , Volume 118 , Issue 2 , April 1997 , pp. 119 - 124
Copyright
© 1997 Cambridge University Press