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The pathogenic properties of Fusobacterium and Bacteroides species from wallabies and other sources

Published online by Cambridge University Press:  19 October 2009

G. R. Smith ,
Janet C. Oliphant  and
R. Parsons
G. R. Smith
Affiliation:
Nuffield Laboratories of Comparative Medicine, Institute of Zoology, The Zoological Society of London, Regent's Park, London NWl 4RY
Janet C. Oliphant
Affiliation:
Nuffield Laboratories of Comparative Medicine, Institute of Zoology, The Zoological Society of London, Regent's Park, London NWl 4RY
R. Parsons
Affiliation:
Nuffield Laboratories of Comparative Medicine, Institute of Zoology, The Zoological Society of London, Regent's Park, London NWl 4RY
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Intracerebral inoculation was more effective than intraperitoneal, intravenous or subcutaneous inoculation as a means of producing lethal infections with Fusobacterium necrophorum in mice. Strains varied in virulence but, of five examined, two had LD50 values as low as ca. 8000 and 14000 viable organisms. Profuse bacterial multiplication in the brain was demonstrated. Intravenous vaccination with a single large dose of heat-killed whole culture or washed bacterial cells failed to protect against intracerebral challenge.

Intracerebral injection of other fusobacteria (F. nucleatum, F. varium and F. necrogenes) and of 22 strains belonging to 10 Bacteroides spp. was without apparent effect on mice, except for a slight transient illness in some animals given B. fragilis. This organism (five strains) differed from the other Bacteroides spp. tested, which included eight strains belonging to the fragilis group, in being eliminated more slowly from the mouse brain – a point that may be relevant to the special pathogenicity of B. fragilis in endogenous infections in man. There was no evidence that B. fragilis multiplied in the brain or that intravenous vaccination with a large dose of heat-killed homologous culture affected the rate at which it was eliminated.

Type
Research Article
Information
Journal of Hygiene , Volume 92 , Issue 2 , April 1984 , pp. 165 - 175
Copyright
Copyright © Cambridge University Press 1984

References

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