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On the Nature of Bacterial Lag

Published online by Cambridge University Press:  15 May 2009

William Jas. Penfold
William Jas. Penfold
Affiliation:
(From the Bacteriological Department, Lister Institute, London.)
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(1) If B. coli be subcultured into another sample of the same medium when growing at full pace, it will continue to grow at the same pace.

(2) If the maximum rate of growth be interrupted by a short application of cold, growth will recommence without lag on the temperature being raised. If the cold be long continued, lag will tend to reappear.

(3) Differences in the size of inoculum have practically no effect on lag in the case of large inoculums, in the case of small ones, on the other hand, diminution of the seeding has the effect of lengthening lag, and this lengthening effect is more marked the smaller the seedings become.

(4) Lowering the temperature lengthens the lag. The effect is very similar to the effect on growth.

(5) The older a parent culture (within limits) the longer the lag.

(6) The length of lag varies with the medium even if adaptation has been arranged for beforehand.

(7) Heat-stable products in B. coli cultures on peptone water have, in the case of overnight cultures, but little effect on lag.

(8) After washing the bacteria for two hours with saline in order to remove possible inhibiting agents, it was found that the lag, on subculture, still occurred and was indeed slightly longer.

(9) If a peptone water culture of B. coli be centrifuged, it is found that the few bacteria remaining in the supernatant commence to grow again at a quick rate but not without a period of lag.

Type
Research Article
Information
Journal of Hygiene , Volume 14 , Issue 2 , July 1914 , pp. 215 - 241
Copyright
Copyright © Cambridge University Press 1914

References

REFERENCES

Buchner, Longard and Riedlin, (1887). Ueber die Vermehrungsgeschwindigkeit der Bakterien. Centralbl. f. Bakteriol. II. 1.Google Scholar
Chick, H. (1912). The Bactericidal Properties of Blood Serum. Journ. of Hygiene., XII. 414.Google Scholar
Coplans, M. (1909, 1910). Influences affecting the growth of Micro-Organisms—Latency: Inhibition: Mass action. Journ. of Pathology., XIV.Google Scholar
Eijkmann, (1904). Ueber thermolabile Stoffwechselproducte als Ursache der natürlichen Wachstumhemmung der Microorganismen. Centralbl. f. Bakteriol. Abt. I, Originale, XXXVII. 436.Google Scholar
Lane-Claypon, (1909). Multiplication of Bacteria and the Influence of Temperature and some other conditions thereon. Journ. of Hygiene., IX.Google Scholar
Müller, Max (1895). Ueber den Einfluss von Fieber temperaturen auf die Wachstumgeschwindigkeit und die Virulenz des Typhus Bacillus. Zeitschr. f. Hygiene und Infektionskr. XX. 245.Google Scholar
Rahn, (1906). Ueber den Einfluss der Stoffwechselprodukte auf das Wachstum der Bacterien. Centralbl. f. Bakteriol. Abt. II, Originale, XVI.Google Scholar