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Numerical analysis of SDS–PAGE protein patterns of Serratia marcescens: a comparison with other typing methods

Published online by Cambridge University Press:  15 May 2009

B. Holmes ,
M. Costas  and
L. L. Sloss
B. Holmes
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, England
M. Costas
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, England
L. L. Sloss
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, England
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Twenty-five cultures comprising 18 clinical isolates of Serratia marcescens from two hospitals, the type strain of S. marcescens, two reference strains of S. marinorubra, the type or a reference strain of three other Serratia species and a reference strain of undetermined species, were characterized by one-dimensional sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) of whole-cell proteins. The protein patterns were highly reproducible and were used as the basis of a numerical analysis which divided the clinical isolates into eight protein types. Comparison with O-serotyping indicated that the level of discrimination by SDS–PAGE was similar. As with O-serotyping, a secondary scheme, such as phage typing, is necessary to differentiate strains of the same protein type. We conclude that high-resolution SDS–PAGE of proteins provides an effective adjunct to other methods for typing isolates of S. marcescens.

Type
Special Article
Information
Epidemiology & Infection , Volume 105 , Issue 1 , August 1990 , pp. 107 - 117
Copyright
Copyright © Cambridge University Press 1990

References

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