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Neutral-Red in the Routine Examination of Water

Published online by Cambridge University Press:  15 May 2009

Ernest E. Irons
Ernest E. Irons
Affiliation:
Bacteriological Laboratory of the University of Chicago
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The use of neutral-red culture media has recently been suggested for the detection of B. coli in water supplies. Rothberger (1) grew a number of organisms on media containing various organic dyes, and found that while some species were able to bring about a reduction of the colouring matter in the medium, others produced no change. This difference in reaction was particularly marked in the case of B. typhosus and B. coli in media containing neutral-red. B. coli casused the reduction of the neutral-red to yellow with green fluorescence, whilst B. typhosus produced no change of colour beyond an occasional fading of the red. After testing a number of races of B. coli and B. typhosus, Rothberger proposed neutral-red media for the differentiation of the two organisms. Scheffler (2) confirmed in the main the work of Rothberger, and further observed that the reaction is by no means specific for B. coli. In addition to a number of bacterial species not commonly found in water, which gave the reaction, Scheffler found 3 of 13 organisms in spring and river waters, and 8 of 18 intestinal organisms from man which, though not belonging to the colon group, gave the neutral-red reaction.

Type
Research Article
Information
Journal of Hygiene , Volume 2 , Issue 3 , July 1902 , pp. 314 - 319
Copyright
Copyright © Cambridge University Press 1902

References

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