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Monoclonal antibodies directed against the flagella of Campylobacter jejuni: cross-reacting and serotypic specificity and potential use in diagnosis

Published online by Cambridge University Press:  19 October 2009

D. G. Newell
D. G. Newell
Affiliation:
Experimental Pathology Laboratory, P. H. L. S., Centre for Applied Microbiology and Research, Porton Down, Salisbury SP4 0JG
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Nine monoclonal antibodies directed against the flagella of Campylobacter jejuni strain 81116 have been investigated for serotypic and cross-reacting activity using a panel of 17 Penner serotype strains of C. jejuni. Four monoclonal antibodies were exclusively specific for serotype-6 strains, which was the serotype of C. jejuni strain 81116. Two monoclonal antibodies cross-reacted with all the flagellated strains of C jejuni tested. One of these cross-reacting monoclonal antibodies, CF5, was found to react with all other Campylobacter species except C. sputorum bulbulus but it did not react with other bacterial entcropathogens. An antigencapture ELISA technique was established, using this monoclonal antibody, which could detect flagellar antigen in human faecal material. These anti-flagella monoclonal antibodies therefore may be valuable in the diagnosis and serotyping of C. jejuni in clinical material.

Type
Research Article
Information
Journal of Hygiene , Volume 96 , Issue 3 , June 1986 , pp. 377 - 384
Copyright
Copyright © Cambridge University Press 1986

References

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