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Molecular typing of Acinetobacter baumannii–Acinetobacter calcoaceticus complex isolates from endemic and epidemic nosocomial infections

Published online by Cambridge University Press:  15 May 2009

P. Ratto ,
D. O. Sordelli ,
E. Abeleira ,
M. Torrero  and
M. Catalano
P. Ratto
Affiliation:
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina
D. O. Sordelli
Affiliation:
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina
E. Abeleira
Affiliation:
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina
M. Torrero
Affiliation:
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina
M. Catalano*
Affiliation:
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina
*
*Mariana Catalo, Departamento de Microbiología FCM-UBA, Paraguay 2155 P-12, (1121) Buenos Aires, Argentina.
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Ribotype, biotype and resistance phenotype were used to characterize 37 Acinetobacter baumannii–A. calcoaceticus complex isolates responsible for nosocomial infections in Buenos Aires. Nineteen isolates were recovered from endemic infections at 2 hospitals and 18 represent an intensive care unit outbreak that occurred in a third hospital. By ribotyping isolates were classified into five different clones of A. baumannii biotype 2, 3 of A. baumannii biotype 9, and 3 of Acinetobacter genospecies 13. Combination of the three epidemiological markers permitted categorization of 18 outbreak isolates into four probable strains: 2 A. baumannii biotype 2. named type I. and II, and 2 A. baumannii biotype 9. Type I (15 isolates) was the most prevalent strain at one hospital and was responsible for the outbreak. In conclusion, combined analysis of biotypes, resistance phenotypes, and ribotypes was an accurate approach for epidemiologic investigation of A. baumannii. Furthermore, ribotyping discriminated Acinetobacter genospecies 13 isolates which were phenotypically difficult to type.

Type
Special Article
Information
Epidemiology & Infection , Volume 114 , Issue 1 , February 1995 , pp. 123 - 132
Copyright
Copyright © Cambridge University Press 1995

References

REFERENCES

1.Bouvet, PJM, Grimont, PAD. Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov., and amended descriptions of Acinetobacter calcoaceticus and Acinetobacter Iwoffi. Int J Syst Bacteriol 1986; 36: 228–40.CrossRefGoogle Scholar
2.Bouvet, PJM, Jeanjean, S, Vieu, JP, Dijkshoorn, L. Species, biotype, and bacteriophage type determinations compared with envelope protein profiles for typing Acinetobacter strains. J Clin Microbiol 1990; 28: 170–6.CrossRefGoogle ScholarPubMed
3.Bouvet, PJM, Jeanjean, S. Delineation of new proteolytic genomic species in the genus Acinetobacter. Res Microbiol 1989; 140: 291–9.CrossRefGoogle ScholarPubMed
4.Bouvet, PJM, Grimont, PAD. Identification and biotyping of clinical isolates of Acinetobacter. Ann Inst Pasteur Microbiol. 1987; 138: 569–78.CrossRefGoogle ScholarPubMed
5.Crombach, WHJ, Dijkshoorn, L, van Noort-Klaasen, M, Niessen, J, van Knippenberg-Gordebeke, G. Control of an epidemic spread of multiresistant strain of Acinetobacter calcoaceticus in a hospital. Care Med 1989; 15: 166–70.Google ScholarPubMed
6.Dijkshoorn, L, Aucken, HM, Gerner-Smidt, P, Kaufmann, ME, Ursing, J.Pitt, TL. Correlation of typing methods for Acinetobacter isolates from hospital outbreak. J Clin Microbiol 1993; 31: 702–5.CrossRefGoogle Scholar
7.Jolly-Guillou, ML, Bergogne-Berezin, E, Vieu, JP. A study of the relationships between antibiotic resistance phenotypes, phage-typing and biotyping of 117 clinical isolates of Acinetobacter spp. J Hosp Infect 1990; 16: 49–58.CrossRefGoogle Scholar
8.Tjernberg, I, Lindh, E, Ursing, J. A quantitative bacterial dot–blot method for DNA–DNA hybridization and its correlation to hydroxyapatite method. Curr Microbiol 1987; 18: 77–81.CrossRefGoogle Scholar
9.Gerner-Smidt, P, Tjernberg, I, Ursing, J. Reliability of phenotypic tests for identification of Acinetobacter species. J Clin Microbiol 1991; 29: 277–82.CrossRefGoogle ScholarPubMed
10.Gerner-Smidt, P. Ribotyping of the Acinetobacter calcoaceticus–Acinetobacter baumannii complex. J Clin Microbiol 1992; 30: 2680–5.CrossRefGoogle ScholarPubMed
11.Gurner, E, Kropec, A, Huebner, J, Altwegg, M, Daschner, F. Ribotyping of Pseudomonas aeruginosa strains from surgical intensive care patients. J Infect Dis 1993; 167: 1216–20.Google Scholar
12.Andrew, HJ. Acinetobacter bacteriocin typing. J Hosp Infect 1986; 7: 169–75.CrossRefGoogle Scholar
13.Traub, WH. Acinetobacter baumannii serotyping for delineation of outbreaks of nosocomial cross-infection. J Clin Microbiol 1989; 27: 2713–6.CrossRefGoogle ScholarPubMed
14.Vila, J, Almela, M, Jimenez, MT de Anta. Laboratory investigation of hospital outbreak caused by two different multiresistant Acinetobacter calcoaceticus subsp. anitratus strains. J Clin Microbiol 1989; 27: 1086–9.CrossRefGoogle ScholarPubMed
15.Seifert, H, Schulze, A, Baginski, R, Pulverer, G. Plasmid DNA fingerprinting of Acinetobacter species other than Acinetobacter baumannii. J Clin Microbiol 1994; 32: 82–6.CrossRefGoogle ScholarPubMed
16.Gouby, A, Carles-Nurit, MJ, Bouziges, N, Bourg, G, Mesnard, R, Bouvet, PJM. Use of pulsed-field gel electrophoresis for investigation of hospital outbreak of Acinetobacter baumannii. J Clin Microbiol 1992; 30: 1588–91.CrossRefGoogle ScholarPubMed
17.National Committee for Clinical Laboratory Standards. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7-A2. National Committee for Clinical Laboratory Standards. Vilanova, Pennsylvania, 1990.Google Scholar
18.Sambrook, J, Fritsch, EF, Maniatis, T. Molecular cloning: a laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory, 1989.Google Scholar
19.Emori, GT, Robert, P. Overview of nosocomial infections, including the role of the microbiology laboratory. Clin Microbiol Rev 1993; 6: 428–42.CrossRefGoogle ScholarPubMed
20.Tenover, FC, Arbeit, R, Archer, G et al. , Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus. J Clin Microbiol 1994; 32: 407–15.CrossRefGoogle ScholarPubMed
21.Seifert, H, Schulze, A, Baginski, R, Pulverer, G. Comparison of four different methods for epidemiological typing of Acinetobacter baumannii. J Clin Microbiol 1994; 32: 1816–9.CrossRefGoogle ScholarPubMed