Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-08T06:33:10.039Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular characterization and antibiotic susceptibility of Vibrio cholerae non-O1

Published online by Cambridge University Press:  15 May 2009

A. Dalsgaard ,
O. Serichantalergs ,
C. Pitarangsi  and
P. Echeverria
A. Dalsgaard
Affiliation:
Laboratory of Fish Diseases, Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, Denmark
O. Serichantalergs
Affiliation:
Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
C. Pitarangsi
Affiliation:
Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
P. Echeverria
Affiliation:
Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A collection of 64 clinical and environmental Vibrio cholerae non-O1 strains isolated in Asia and Peru were characterized by molecular methods and antibiotic susceptibility testing. All strains were resistant to at least 1 and 80% were resistant to two or more antibiotics. Several strains showed multiple antibiotic resistance (≥ three antibiotics). Plasmids most often of low molecular weight were found in 21/64 (33%) strains. The presence of plasmids did not correlate with antibiotic resistance or influence ribotype patterns. In colony hybridization studies 63/64 (98%) V. cholerae non-O1 strains were cholera toxin negative, whereas only strains recovered from patients were heat-stable enterotoxin positive. Forty-seven Bgl I ribotypes were observed. No correlation was shown between ribotype and toxin gene status. Ribotype similarity was compared by cluster analysis and two main groups of 13 and 34 ribotypes was found. Ribotyping is apparently a useful epidemiological tool in investigations of V. cholerae non-O1 infections.

Type
Special Article
Information
Epidemiology & Infection , Volume 114 , Issue 1 , February 1995 , pp. 51 - 63
Copyright
Copyright © Cambridge University Press 1995

References

REFERENCES

1.Kaper, J, Lockman, H, Colwell, RR, Joseph, SW. Ecology, serology, and enterotoxin production of Vibrio cholerae in Chesapeake bay. Appl Environ Microbiol 1979; 37: 91–103.CrossRefGoogle ScholarPubMed
2.Kaysner, CA, Abeyta, CJR, Wekell, MM, Depaola, A Jr, Stott, RF, Leitch, JM. Incidence of Vibrio cholerae from estuaries of the United States West coast. Appl Environ Microbiol 1987; 53: 1344–8.CrossRefGoogle ScholarPubMed
3.Adkins, HJ, Escamilla, J, Santiago, LT, Rañoa, C, Echeverria, P, Cross, JH. Two year survey of etiologic agents of diarrheal disease at San Lazaro hospital, Manila, Republic of the Philippines. J Clin Microbiol 1987; 25: 1143–7.CrossRefGoogle Scholar
4.Desenclos, JA, Klontz, KC, Wolfe, LE, Hoecheri, S. The risk of Vibrio illness in the Florida raw oyster eating population, 1981–1988. Am J Epidemiol 1991; 134: 290–7.CrossRefGoogle ScholarPubMed
5.Piersimoni, C, Morbiducci, V, Scalise, G. Non-O1 Vibrio cholerae gastroenteritis and bacteriaemia. Lancet 1991; 337: 791–2.CrossRefGoogle Scholar
6.Bagchi, K, Echeverria, P, Arthur, JD, Sethabutr, O, Serichantalergs, O, Hoge, CW. Epidemic of diarrhoea caused by Vibrio cholerae non-O1 that produced heat-stable toxin among Khmers in a camp in Thailand. J Clin Microbiol 1993; 31: 1315–7.CrossRefGoogle Scholar
7.Pitrak, LD, Gindorf, JD. Bacteriaemic cellulitis caused by non-serogroup O1 Vibrio cholerae acquired in a freshwater inland lake. J Clin Microbiol 1989; 27: 2874–6.CrossRefGoogle Scholar
8.W.H.O. Epidemic diarrhoea due to Vibrio cholerae non-O1. Weekly Epidemiol Rec 1993; 68: 141–2.Google Scholar
9.Newland, JW, Voll, MJ, McNicol, LA. Serology and plasmid carriage in Vibrio cholerae. Can J Microbiol 1984; 30: 1149–56.CrossRefGoogle ScholarPubMed
10.Amaro, C, Aznar, R, Garay, E, Alcaide, E. R plasmids in environmental Vibrio cholerae non-O1 strains. Appl Environ Microbiol 1988; 54: 2771–6.CrossRefGoogle ScholarPubMed
11.Barja, JL, Santos, Y, Huq, I, Colwell, RR, Toranzo, AE. Plasmids and factors associated with virulence in environmental isolates of Vibrio cholerae non-O1 in Bangladesh. J Med Microbiol 1990; 33: 107–14.CrossRefGoogle ScholarPubMed
12.Grimont, F, Grimont, PAD. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur/Microbiol 1986; 137B: 165–75.CrossRefGoogle ScholarPubMed
13.Popovic, T, Bopp, CA, Olsvik, Ö, Wachsmuth, K. Epidemiologic application of a standardized ribotype scheme for Vibrio cholerae O1. J Clin Microbiol 1993; 31: 2474–82.CrossRefGoogle ScholarPubMed
14.Aznar, R, Ludwig, W, Schleifer, K-H. Ribotyping and randomly amplified polymorphic DNA analysis of Vibrio vulnificus biotypes. System Appl Microbiol 1993; 16: 303–9.CrossRefGoogle Scholar
15.Pedersen, K, Larsen, JL. rRNA gene restriction patterns of Vibrio anguillarum serogroup O1. Dis Aquat Org 1993; 16: 121–6.CrossRefGoogle Scholar
16.Hoge, CW, Sethabuth, O, Bodhidatta, L, Echeverria, P, Robertson, DC, Morris, JG Jr. Use of a synthetic oligonucleotide probe to detect strains of non serovar O1 Vibrio cholerae carrying the gene for heat-stable enterotoxin (NAG-ST). J Clin Microbiol 1990; 28: 1473–6.CrossRefGoogle ScholarPubMed
17.Morris, GJ Jr., Takeda, T, Tall, BD et al. , Experimental non-O group 1 Vibrio cholerae gastroenteritis in humans. J Clin Invest 1990; 85: 697–705.CrossRefGoogle ScholarPubMed
18.Colwell, RR. Vibrios in the environment. New York: John Wiley & Sons, 1984.Google Scholar
19.Baumann, P, Schubert, RHW. Bergey's manual of systematic bacteriology, vol. 1. Baltimore: The Williams & Wilkins Co., 1984: 516–50.Google Scholar
20.Sakazaki, R. Bacteriology of vibrio and related organisms, In: Barua, D, Greenough, WR, eds. Cholera, New York: Plenum Publishing Company, 1992: 37–55.Google Scholar
21.Bauer, AW, Kirby, MM, Sherris, JC, Turch, M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Path 1966; 36: 493–6.CrossRefGoogle ScholarPubMed
22.Kado, CI, Liu, ST. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981; 145: 1365–73.CrossRefGoogle ScholarPubMed
23.Olsen, JE, Larsen, JLL. Restriction fragment length polymorphism of the Vibrio anguillarum serovar O1 virulence plasmid. Appl Environ Microbiol 1990; 56: 3130–2.CrossRefGoogle ScholarPubMed
24.Wright, AC, Guo, Y, Johnson, JA, Nataro, JP, Morris, JG Jr. Development and testing of a non-radioactive DNA oligonucleotide probe that is specific for Vibrio cholerae cholera toxin. J Clin Microbiol 1992; 30: 2302–6.CrossRefGoogle Scholar
25.Ogawa, A, Kato, J, Watanabe, H, Nair, BG, Takeda, T. Cloning and nucleotide sequence of a heat-stable enterotoxin gene from Vibrio cholerae non-O1 isolated from a patient with traveler's diarrhea. Infect Immun 1990; 58: 3325–9.CrossRefGoogle ScholarPubMed
26.Arita, M, Takeda, T, Honda, T, Miwatani, T. Purification and characterization of Vibrio cholerae non-O1 heat-stable enterotoxin. Infect Immun 1986; 52: 45–9.CrossRefGoogle ScholarPubMed
27.Maniatis, T, Fritsch, EF, Sambrook, J. In: Molecular cloning: a laboratory manual. Cold Spring Harbor: Cold Spring Harbor Laboratory, 1982: 122–3.Google Scholar
28.Maas, R. An improved colony hybridization method with a significant increased sensitivity for detection of single genes. Plasmid 1983; 10: 296–8.CrossRefGoogle Scholar
29.Faruque, SM, Albert, J. Genetic relation between Vibrio cholerae O1 strains in Ecuador and Bangladesh. Lancet 1992; 339: 740–1.CrossRefGoogle ScholarPubMed
30.Southern, EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975; 98: 503–17.CrossRefGoogle ScholarPubMed
31.Olsen, JE, Brown, DJ, Baggesen, DL, Bisgaard, M. Biochemical and molecular characterization of Salmonella enterica serovar berta, and comparison of methods for typing. Epidemiol Infect 1992; 108: 243–60.CrossRefGoogle ScholarPubMed
32.Hunter, PR, Gaston, MA. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J Clin Microbiol 1988; 26: 2465–6.CrossRefGoogle ScholarPubMed
33.Blanc, DS, Siegrist, HH, Sahli, R, Francioli, P. Ribotyping of Pseudomonas aeruginosa: discriminatory power and usefulness as a tool for epidemiological studies. J Clin Microbiol 1993; 31: 71–7.CrossRefGoogle ScholarPubMed
34.Albert, MJ, Siddique, AK, Islam, MS et al. , Large outbreak of clinical cholera due to Vibrio cholerae non-O1 in Bangladesh. Lancet 1993; 341: 704–5.CrossRefGoogle ScholarPubMed
35.Shehabi, AA, Drexler, H, Richardson, SH. Virulence mechanisms associated with clinical isolates of non-O1 Vibrio cholerae. Zbl Bakt Hyg 1986; 261: 232–9.Google ScholarPubMed
36.Ramamurthy, T, Bag, PK, Pal, A et al. , Virulence patterns of Vibrio cholerae non-O1 strains isolated from hospitalised patients with acute diarrhoea in Calcutta, India. J Med Microbiol 1993; 39: 310–7.CrossRefGoogle ScholarPubMed
37.Koblavi, S, Grimont, F, Grimont, PAD. Clonal diversity of Vibrio cholerae O1 evidenced by rRNA gene restriction patterns. Res Microbiol 1990; 141: 645–57.CrossRefGoogle ScholarPubMed