Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-04T18:59:03.808Z Has data issue: false hasContentIssue false

A modification of the growth-inhibition test and its use for detecting Mycoplasma mycoides var. mycoides

Published online by Cambridge University Press:  15 May 2009

G. Davies
Affiliation:
East African Veterinary Research Organization, Muguga, Kenya
W. C. S. Read
Affiliation:
East African Veterinary Research Organization, Muguga, Kenya
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A modification of the growth-inhibition test for identifying Mycoplasma species is described. The modification simplifies the screening of a large number of strains for one species. Experiments showed that it was effective and specific when used to identify M. mycoides var. mycoides. Its use in studying the epidemiology of contagious bovine pleuropneumonia is discussed.

This work was financed in part by the United States of America Agency for International Development under the terms of the CCTA/AID Joint Project 16 for research on contagious bovine pleuropneumonia.

We wish to thank Dr R. H. Leach for the various bovine mycoplasma cultures.

Type
Original Articles
Copyright
Copyright © Cambridge University Press 1968

References

Brown, R. D., Gourlay, R. N. & Macleod, A. K. (1965). The production of T1 broth culture contagious bovine pleuropneumonia vaccine. Bull. epizoot. Dis. Afr. 13, 149.Google ScholarPubMed
Bygrave, A. C., Moulton, J. E. & Shifrine, M. (1968). Clinical, serological and pathological findings in an outbreak of contagious bovine pleuropneumonia. Bull. epizoot. Dis. Afr. (in the press).Google Scholar
Clyde, W. A. (1964). Mycoplasma species identification based upon growth-inhibition by specific antisera. J. Immun. 92, 958.CrossRefGoogle ScholarPubMed
Edward, D. G. FF. & Fitzgerald, W. A. (1954). Inhibition of growth of pleuropneumonia-like organisms by antibody. J. Path. Bact. 68, 23.CrossRefGoogle ScholarPubMed
Report, F.A.O. (1967). Report of the third meeting of the FAO/OIE/OAU Expert Panel on contagious bovine pleuropneumonia. Khartoum.Google Scholar
Gourlay, R. N. (1964). Antigenicity of Mycoplasma mycoides. I. Examination of body fluids from cases of contagious bovine pleuropneumonia. Res. vet. Sci. 5, 473.CrossRefGoogle Scholar
Huijmans-Evers, A. G. M. & Ruys, A. C. (1956). Micro-organisms of the pleuropneumonia group (family of Mycoplasmataceae) in man. II. Serological identification and discussion of pathogenicity. Antonie van Leeuwenhoek 22, 377.Google Scholar
Leach, R. H. (1967). Comparative studies of mycoplasma of bovine origin. Ann. N.Y. Acad. Sci. 143, 305.CrossRefGoogle ScholarPubMed
Lemcke, R. M. (1964). The serological differentiation of Mycoplasma strains (pleuro-pneumonia-like organisms) from various sources. J. Hyg., Camb. 62, 199.CrossRefGoogle ScholarPubMed
Longley, E. O. (1951). Contagious Bovine Pleuropneumonia—a Study of the Disease in Nigeria. Colon. Publ. no. 7, London: H.M.S.O.Google Scholar
Newing, R. & Macleod, A. K. (1958). Rep. E. Afr. vet. Res. Org. 1956–7 Available from the Librarian, E.A.V.R.O.Google Scholar
Piercy, S. E. & Knight, G. T. (1957). The preparation, titration and challenge of avianised bovine pleuropneumonia vaccines. Bull. epizoot. Dis. Afr. 5, 161.Google Scholar
Priestley, F. W. (1952). Observations on immunity to CBPP with special reference to the bacteriocidal action of blood. Brit. vet. J. 108, 153.CrossRefGoogle Scholar
Razin, S., Cosenza, B. J. & Tourtellotte, M. E. (1967). Filamentous growth of mycoplasma. Ann. N.Y. Acad. Sci. 143, 66.CrossRefGoogle ScholarPubMed
Reich, P. R., Somerson, N. L., Rose, J. A. & Weissman, S. M. (1966). Genetic relatedness among mycoplasmas as determined by nucleic acid homology. J. Bact. 91, 153.CrossRefGoogle ScholarPubMed
Stanbridge, E. & Hayflick, L. (1967). Growth-inhibition test for identification of mycoplasma species utilising dried antiserum-impregnated paper discs. J. Bact. 93, 1392.CrossRefGoogle ScholarPubMed
Turner, A. W. (1959). Infectious Diseases of Animals (by Stableforth and Galloway), p. 440. London: Butterworths Ltd.Google Scholar
Turner, A. W. (1962). Detection of Mycoplasma mycoides antigen and antibody by means of precipitin tests as aids to diagnosis of bovine contagious pleuropneumonia. Aust. vet. J. 38, 335.CrossRefGoogle Scholar