Hostname: page-component-745bb68f8f-l4dxg Total loading time: 0 Render date: 2025-01-08T14:34:26.477Z Has data issue: false hasContentIssue false
  • English
  • Français

The measurement of the D-antigen in poliovirus preparations

Published online by Cambridge University Press:  15 May 2009

A. J. Beale  and
P. J. Mason
A. J. Beale
Affiliation:
Virus Research Unit, Glaxo Laboratories Ltd, Sefton Park, Stoke Poges, Bucks
P. J. Mason
Affiliation:
Virus Research Unit, Glaxo Laboratories Ltd, Sefton Park, Stoke Poges, Bucks
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A simple micro-method of measuring ‘D-’ and ‘C-’ antigen contents of poliovirus preparations is described. The D-antigen of poliovirus is almost certainly the component of the virus involved in eliciting neutralizing antibodies, and the D-antigen assay thus becomes a simple in vitro method for assessing the antigenic potency of poliovaccines and for investigating the influence of various factors upon the antigen. The value of the technique in following the concentration and purification of poliovirus preparations is illustrated in the studies reported in the accompanying paper by Fantes (1962). The results of experiments designed to test the validity of the idea that D-antigen is the antigen eliciting neutralizing antibodies are discussed by Beale (1961).

Type
Research Article
Information
Journal of Hygiene , Volume 60 , Issue 1 , March 1962 , pp. 113 - 121
Copyright
Copyright © Cambridge University Press 1962

References

REFERENCES

Baron, S. (1957). Ultracentrifuge concentration of poliovirus and effect of calf serum and gelatin. Proc. Soc. exp. Biol., N.Y., 95, 760.CrossRefGoogle ScholarPubMed
Beale, A. J. (1961). Lancet, ii, 1166.CrossRefGoogle Scholar
Crowle, A. J. (1958). A simplified micro double-diffusion agar precipitin technique. J. Lab. clin. Med. 52, 784.Google ScholarPubMed
Fantes, K. H. (1962). J. Hyg., Camb., 60, 123.CrossRefGoogle Scholar
Farrell, L. N., Wood, W., MacMorine, H. G., Shimada, F. T. & Graham, D. G. (1955). Preparation of poliomyelitis virus for the production of vaccine for the 1954 field trial. Canad. J. publ. Hlth, 46, 265.Google ScholarPubMed
Hummeler, K. & Hamparian, V. V. (1958). Studies on the complement-fixing antigens of poliomyelitis. I. Demonstration of type and group-specific antigens in native and heated viral preparations. J. Immunol. 81, 499.CrossRefGoogle ScholarPubMed
Kohn, J. (1959). A simple method for the concentration of fluids containing protein. Nature, Lond., 183, 1055.CrossRefGoogle ScholarPubMed
Le Bouvier, G. L. (1959). Poliovirus D and C antigens: their differentiation and measurement by precipitation in agar. Brit. J. exp. Path., 40, 452.Google ScholarPubMed
Le Bouvier, G. L., Schwerdt, C. E. & Schaffer, F. L. (1957). Specific precipitates in agar with purified poliovirus. Virology, 4, 590.CrossRefGoogle ScholarPubMed
Roizman, B., Höpken, W. & Mayer, M. M. (1958). Immunochemical studies of poliovirus. II. Kinetics of the formation of infectious and non-infectious Type 1 poliovirus in three cell strains of human derivation. J. Immunol. 80, 386.CrossRefGoogle Scholar
Schwerdt, C. E. & Schaffer, F. L. (1956). Purification of poliomyelitis viruses propagated in tissue culture. Virology, 2, 665.CrossRefGoogle Scholar
Warren, J., Hare, J. D. & Goldman, C. (1959). Complement-fixing antigens of living and inactivated poliovirus. II. Complement-fixing activity of poliomyelitis vaccine and its relations to immunogenic potency. Amer. J. publ. Hlth, 49, 53.CrossRefGoogle ScholarPubMed