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Longitudinal monitoring of the dynamics of infections due to Bartonella species in UK woodland rodents

Published online by Cambridge University Press:  30 May 2001

R. J. BIRTLES ,
S. M. HAZEL ,
M. BENNETT ,
K. BOWN ,
D. RAOULT  and
M. BEGON
R. J. BIRTLES
Affiliation:
Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK Unité des Rickettsies, UPRESA 6020, Faculté de Médecine, 27 Boulevard Jean Moulin, 13385 Marseille cédex 5, France
S. M. HAZEL
Affiliation:
Population Biology Research Group, School of Biological Sciences and Centre for Comparative Infectious Diseases, University of Liverpool, PO Box 147, Liverpool L69 3BX, UK Department of Veterinary Clinical Science and Animal Husbandry and Centre for Comparative Infectious Diseases, University of Liverpool, Leahurst, Chester High Road, Cheshire CH64 7TE, UK
M. BENNETT
Affiliation:
Department of Veterinary Clinical Science and Animal Husbandry and Centre for Comparative Infectious Diseases, University of Liverpool, Leahurst, Chester High Road, Cheshire CH64 7TE, UK
K. BOWN
Affiliation:
Population Biology Research Group, School of Biological Sciences and Centre for Comparative Infectious Diseases, University of Liverpool, PO Box 147, Liverpool L69 3BX, UK Department of Veterinary Clinical Science and Animal Husbandry and Centre for Comparative Infectious Diseases, University of Liverpool, Leahurst, Chester High Road, Cheshire CH64 7TE, UK
D. RAOULT
Affiliation:
Unité des Rickettsies, UPRESA 6020, Faculté de Médecine, 27 Boulevard Jean Moulin, 13385 Marseille cédex 5, France
M. BEGON
Affiliation:
Population Biology Research Group, School of Biological Sciences and Centre for Comparative Infectious Diseases, University of Liverpool, PO Box 147, Liverpool L69 3BX, UK Department of Veterinary Clinical Science and Animal Husbandry and Centre for Comparative Infectious Diseases, University of Liverpool, Leahurst, Chester High Road, Cheshire CH64 7TE, UK
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Abstract

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Blood samples were repeatedly collected from 12 sympatric woodland rodents over a 12-month period and DNA extracts from each were incorporated into a bartonella-specific PCR targeting a fragment of the 16S/23S rRNA intergenic spacer region (ISR). The composition of each amplicon was analysed using restriction enzyme analysis (REA) and base sequence comparison. Bartonella DNA was detected in 70 of 109 samples. Eleven samples contained DNA derived from more than one strain. Sequence analysis of 62 samples found 12 sequence variants (ISR genotypes) that were provisionally assigned to 5 different species, 2 of which were newly recognized. Up to five different species were detected in each animal. On about two-thirds of occasions, a species detected 1 month was not there the next, but never was a genotype superseded by another of the same species. However, a genotype could be re-encountered months later in the same animal, even if interim samples contained other genotypes. Our results suggest that although most animals are bacteraemic most of the time, specific infections are often superseded and that a complex and dynamic epidemiology of bartonella bacteraemias exists in woodland rodents.

Type
Research Article
Information
Epidemiology & Infection , Volume 126 , Issue 2 , April 2001 , pp. 323 - 329
Copyright
© 2001 Cambridge University Press