Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-24T19:02:23.207Z Has data issue: false hasContentIssue false
  • English
  • Français

The late detection of respiratory syncytial virus in cells of respiratory tract by immunofluorescence

Published online by Cambridge University Press:  15 May 2009

P. S. Gardner ,
Joyce McQuillin  and
Rosemary McGuckin
P. S. Gardner
Affiliation:
Department of Virology, Royal Victoria Infirmary, and University of Newcastle upon Tyne, Newcastle upon Tyne, 1
Joyce McQuillin
Affiliation:
Department of Virology, Royal Victoria Infirmary, and University of Newcastle upon Tyne, Newcastle upon Tyne, 1
Rosemary McGuckin
Affiliation:
Department of Virology, Royal Victoria Infirmary, and University of Newcastle upon Tyne, Newcastle upon Tyne, 1
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Paired nasopharyngeal secretions were studied in 27 infants infected with respiratory syncytial virus, one taken at onset of illness and one about 7 days later. Both specimens were examined by immunofluorescence and tissue culture for respiratory syncytial virus. In 25 out of 27 (93%) specific fluorescence was still present in cells of the convalescent specimen but was much duller. Virus was difficult to isolate in convalescent specimens; only 8 out of 27 (26 %) proved to be positive. Eight single secretions which were taken late in a respiratory illness were also shown to have this altered fluorescence with absence of virus isolation. Preliminary experiments using antihuman globulin suggest that the findings may be due to the attachment of local secretory antibody to the cells causing ‘blocking’ of staining reaction.

Type
Research Article
Information
Journal of Hygiene , Volume 68 , Issue 4 , December 1970 , pp. 575 - 580
Copyright
Copyright © Cambridge University Press 1970

References

REFERENCES

Gardner, P. S. & McQuillin, J. (1968). The application of the immunofluorescent antibody technique in the rapid diagnosis of respiratory syncytial virus infection. British Medical Journal iii, 340.CrossRefGoogle Scholar
Gardner, P. S., McQuillin, J. & Court, S. D. M. (1970). Speculation on pathogenesis in death from respiratory syncytial virus infection. British Medical Journal i, 327.CrossRefGoogle Scholar
Kim, H. W., Bellanti, J. A., Arrobio, J. O., Mills, J., Brandt, C. D., Chanock, R. M. & Parrott, R. H. (1969). Respiratory syncytial virus neutralizing activity in nasal secretions following natural infection. Proceedings of the Society for Experimental Biology and Medicine 131, 658.CrossRefGoogle ScholarPubMed
McQuillin, J. & Gardner, P. S. (1968). Rapid diagnosis of respiratory syncytial virus infection by immunofluorescent antibody techniques. British Medical Journal i, 602.CrossRefGoogle Scholar
Scott, R. & Gardner, P. S. (1970). Respiratory syncytial virus neutralizing activity in nasopharyngeal secretions. Journal of Hygiene 68, 581.Google ScholarPubMed
Smith, C. B., Purcell, R. H., Bellanti, J. A. & Chanock, R. M. (1966). Protective effect of antibody to parainfluenza type 1 virus. New England Journal of Medicine 275, 1145.CrossRefGoogle ScholarPubMed
Sturdy, P. M., McQuillin, J. & Gardner, P. S. (1969). A comparative study of methods for the diagnosis of respiratory virus infections in childhood. Journal of Hygiene 67, 659.Google ScholarPubMed