Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-03T05:02:00.418Z Has data issue: false hasContentIssue false
  • English
  • Français

Langat virus encephalitis in mice I. The effect of the administration of specific antiserum

Published online by Cambridge University Press:  15 May 2009

H. E. Webb ,
D. G. D. Wight ,
G. S. Platt  and
C. E. G. Smith
H. E. Webb
Affiliation:
St Thomas's Hospital, London, S.E. 1
D. G. D. Wight
Affiliation:
St Thomas's Hospital, London, S.E. 1
G. S. Platt
Affiliation:
Microbiological Research Establishment, Porton, Salisbury, Wilts.
C. E. G. Smith
Affiliation:
Microbiological Research Establishment, Porton, Salisbury, Wilts.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. When mice are infected intraperitoneally with Langat virus only a small proportion develop clinical encephalitis, but all mice have substantial titres of virus in the brain and also incontrovertible histological evidence of encephalitis.

2. When specific antibody is given intraperitoneally or intravenously to mice during the first 3 days after intraperitoneal infection with Langat virus, the viraemia (normally maximal during this period) is depressed, the production of antibody is depressed or delayed, and the incidence of clinical encephalomyelitis is increased significantly.

3. Specific antibody given intraperitoneally or intracerebrally before infection, protects the animals from encephalitis.

4. These findings are discussed in terms of the histology of the central nervous system of the affected mice.

We are very grateful to Miss S. J. Illavia, B.Sc., and to Miss G. E. Fairbairn for their skilled technical assistance; and to Mr S. Peto of the Microbiological Research Establishment for statistical advice.

This work was made possible by a generous grant from the Wellcome Trust and the Endowment Funds of St Thomas's Hospital

Type
Original Articles
Information
Journal of Hygiene , Volume 66 , Issue 3 , September 1968 , pp. 343 - 354
Copyright
Copyright © Cambridge University Press 1968

References

Berge, T. O., Gleiser, C. E. G., Gochenour, W. S., Miesse, M. L. & Tigertt, W. D (1961). Studies on the virus of Venezuelan equine encephalomyelitis: II. Modification by specific immune serum of response of central nervous system of mice. J. Immun. 87, 509–17.CrossRefGoogle ScholarPubMed
Clarke, D. H. & Casals, J. (1958). Techniques for haemagglutination and haemagglutination inhibition with arthropod borne viruses. Am. J. trop. Med. Hyg. 7, 561–73.CrossRefGoogle ScholarPubMed
Holden, P., Muth, D. & Skinner, R. B. (1966). Arbovirus haemagglutination inhibitors in avian sera—inactivation with protamine sulphate. Am. J. Epidem. 84, 67–73.CrossRefGoogle Scholar
Reed, L. J. & Muench, H. (1938). A simple method of estimating fifty per cent endpoints. Am. J. Hyg. 27, 493–7.Google Scholar
Sever, J. L. (1962). Application of a microtechnique to viral serological investigations. J. Immun. 88, 320–9.CrossRefGoogle ScholarPubMed
Webb, H. E. & Smith, C. E. G. (1966). Relation of immune response to development of central nervous system lesions in virus infections of man. Br. med. J. ii, 1179–81.CrossRefGoogle Scholar
Webb, H. E., Wetherley-Mein, G., Smith, C. E. G. & Mcmahon, D. (1966). Leukaemia and neoplastic processes treated with Langat and Kyasanur Forest disease viruses: a clinical and laboratory study of 28 patients. Br. med. J. i, 258–66.CrossRefGoogle Scholar
Webb, H. E., Wight, D. G. D., Wiernik, G., Platt, G. S. & Smith, C. E. G. (1968). Langat virus encephalitis in mice. II. Theeffects of irradiation. J. Hyg., Camb. 66, 355–64.CrossRefGoogle Scholar
Weigand, M. & Hotchin, J. (1961). Studies of lymphocytic choriomeningitis in mice: II. A comparison of the immune status of newborn and adult mice surviving inoculation. J. Immun. 86, 401–6.CrossRefGoogle Scholar