Hostname: page-component-586b7cd67f-t7czq Total loading time: 0 Render date: 2024-11-30T19:00:10.276Z Has data issue: false hasContentIssue false
  • English
  • Français

Laboratory diagnosis of Mycoplasma pneumoniae infection: 3. Detection of IgM antibodies to M. pneumoniae by a modified indirect haemagglutination test

Published online by Cambridge University Press:  15 May 2009

T.-W. Kok ,
B. P. Marmion ,
G. Varkanis ,
D. A. Worswick  and
J. Martin
T.-W. Kok
Affiliation:
Division of Medical Virology, Institute of Medical and Veterinary Science, Adelaide and Department of Pathology, University of Adelaide
B. P. Marmion
Affiliation:
Division of Medical Virology, Institute of Medical and Veterinary Science, Adelaide and Department of Pathology, University of Adelaide
G. Varkanis
Affiliation:
Division of Medical Virology, Institute of Medical and Veterinary Science, Adelaide and Department of Pathology, University of Adelaide
D. A. Worswick
Affiliation:
Division of Medical Virology, Institute of Medical and Veterinary Science, Adelaide and Department of Pathology, University of Adelaide
J. Martin
Affiliation:
Department of Pulmonary Medicine, Adelaide Children's Hospital
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The indirect haemagglutination (IHA) test was compared with the complement-fixation (CF) test for the measurement of antibodies to Mycoplasma pneumoniae. A modification of the IHA was used to measure M. pneumoniae IgM antibodies. Sera were obtained from various groups of patients who were either culture or antigen positive for M. pneumoniae in nasopharyngeal aspirates or who had fourfold or greater increase in CF antibody or a titre ≥ 320.

Type
Research Article
Information
Epidemiology & Infection , Volume 103 , Issue 3 , December 1989 , pp. 613 - 623
Copyright
Copyright © Cambridge University Press 1989

References

REFERENCES

1.Kok, T-W, Varkanis, G, Marmion, BP, Martin, J, Esterman, A.Laboratory diagnosis of Mycoplasma pneumoniae infection. 1. Direct detection of antigen in respiratory exudates by enzyme immunoassay. Epidemiol Infect 1988; 101: 669–84.CrossRefGoogle ScholarPubMed
2.Taylor-Robinson, D, Sobeslavsky, O, Jensen, KE, Senterfit, LB, Chanock, RM.Serologic Response to Mycoplasma pneumoniae Infection. I. Evaluation of immunofluorescence, complement fixation, indirect haemagglutination and tetrazolium reduction inhibition tests for the diagnosis of infection. Am J Epidemiol 1966; 83: 287–98.CrossRefGoogle ScholarPubMed
3.Grayston, JT, Foy, HM, Kenny, G. The epidemiology of mycoplasma infections of the human respiratory tract. In: Hayflick, L, ed. The mycoplasmatales and the L-phase of bacteria. Amsterdam: North-Holland Publishing Co; 1969: 651–82.Google Scholar
4.Clyde, WA Jr M. pneumoniae in the pediatric population. In: mycoplasmas as agents of human disease. Waukegon, Illinois: Windermere Communications Inc; 1985: 51–64.Google Scholar
5.Foy, HM, Cooney, MK, McMahan, R, Grayston, JT.Viral and mycoplasmal pneumonia in a prepaid medical care group during an 8-yr Period. Am J Epidemiol 1973; 97: 93–102.CrossRefGoogle Scholar
6.Dowdle, WR, Robinson, RQ.An indirect hemagglutination test for diagnosis of Mycoplasma pneumoniae infections. Proc Soc Exptl Biol & Med 1964; 116: 947–50.CrossRefGoogle ScholarPubMed
7.Avrameas, S, Ternynck, T.The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochem 1969; 6: 53–66.CrossRefGoogle ScholarPubMed
8.Busolo, F, Tonin, E, Meloni, GA.Enzyme-linked immunosorbent Assay for serodiagnosis of M. pneumoniae infections. J Clin Microbiol 1983; 18: 432–5.CrossRefGoogle Scholar
9.Gardner, PS, McQuillin, J.Rapid virus diagnosis, application of immunofluorescence. Butterworths Publishing Co Ltd., 1974.Google Scholar
10.Hu, PC, Powell, DA, Albright, F, Gardner, DE, Collier, AM, Clyde, WA JrA solid-phase radioimmunoassay for detection of antibodies against Mycoplasma pneumoniae. J Clin Lab Immunol 1983; 11: 209–13.Google ScholarPubMed
11.Coombs, RRA, Easter, G, Matejtschuk, P, Wreghitt, TG.Red-cell IgM-antibody capture assay for the detection of Mycoplasma pneumoniae-specific IgM. Epidemiol Infect 1988; 100: 101–9.CrossRefGoogle ScholarPubMed
12.Biberfeld, G.Antibody responses in Mycoplasma pneumoniae infection in relation to serum immunoglobulins, especially IgM. Acta Path Microbiol Scand (Sect B) 1971; 79: 620–34.Google Scholar
13.Price, PC.Direct radioimmunoassay for the detection of IgM antibodies against Mycoplasma pneumoniae. J Immunol Meth 1980; 32: 261–73.CrossRefGoogle ScholarPubMed
14.Brunner, H, Prescott, B, Greenberg, H, James, WD, Horswood, RL, Chanock, RM.Unexpectedly high frequency of antibody to Mycoplasma pneumoniae in human sera as measured by sensitive techniques. J. Infect Dis 1977; 135: 524–30.CrossRefGoogle Scholar
15.Hu, PC, Juang, C-H, Collier, AM, Clyde, WA.Demonstration of antibodies to Mycoplasma pneumoniae attachment protein in human sera and respiratory secretions. Infect & Imm 1983; 41: 437–9.CrossRefGoogle ScholarPubMed
16.Marmion, BP. The mycoplasmas – new information on their properties and their pathogenicity for man. In: Waterson, AP, ed. Recent advances in mdical microbiology, J & A Churchill Ltd; 1967: 170–253.Google Scholar
17.Somerson, NL, James, WD, Walls, BE, Chanock, RM.Growth of Mycoplasma pneumoniae on a glass surface. Ann NY Acad Sci 1967; 143: 384–9.CrossRefGoogle ScholarPubMed