Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-27T20:49:53.692Z Has data issue: false hasContentIssue false
  • English
  • Français

Isolation of Legionella pneumophila from water supplies: comparison of methods based on the guinea-pig and culture media

Published online by Cambridge University Press:  19 October 2009

R. B. Fitzgeorge  and
P. J. Dennis
R. B. Fitzgeorge
Affiliation:
PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 OJG, UK
P. J. Dennis
Affiliation:
PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 OJG, UK
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The detection of Legionella pneumophila in water via guinea-pig intraperitoneal injection has been compared with direct isolation of these organisms on semiselective BCYEα media. Both techniques were of similar sensitivity, detecting as few as 102L. pneumophila in 10 ml of water. However, at this concentration, detection using guinea-pig intraperitoneal injection was via the indirect parameters of antibody production and immunofluorescent microscopy. Isolation of L. pneumophila from guinea-pig tissue for further investigation required > 107 organisms injected and in this respect is relatively much less sensitive than direct isolation on semiselective BCYEα media. Since L. micdadei and L. longbeachae are inhibited by one of the selective supplements used, other supplements and techniques need to be developed in order to avoid possible inhibition of other Legionella species.

Type
Research Article
Information
Journal of Hygiene , Volume 91 , Issue 2 , October 1983 , pp. 179 - 187
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Dennis, P. J., Taylor, J. A., Fitzgeoroe, R. B., Bartlett, C. L. R. & Barrow, G. I. (1982). Legionella pneumophila in water plumbing systems. Lancet i, 949–951.CrossRefGoogle Scholar
Edelstein, P. H. (1981). Improved semiselective medium for isolation of L. pneumophilafrom contaminated clinical and environmental specimens. Journal of Clinical Microbiology 14, 298–303.CrossRefGoogle ScholarPubMed
Edelstein, P. H., Snitzer, J. B. & Finegold, S. M. (1982). Isolation of L. pneumophilafrom hospital potable water specimens: comparison of direct plating with guinea-pig inoculaton. Journal of Clinical Microbiology 15, 1092–1096.CrossRefGoogle Scholar
Fitzoeoroe, R. B., Baskerville, A., Broster, M., Hambleton, P. & Dennis, P. J. (1983). Aerosol infection of animals with strains of Legionella pneumophilaof different virulence: comparison with intrnperitoneal and intranasal routes of infection. Journal of Hygiene 90, 81–89.Google Scholar
Fliermans, C. B., Cherry, W. B., Orrison, L. H., Smith, S. J., Tison, D. L. & Pope, D. M. (1981). Ecological distribution of Legionella pneumophila. Applied Environmental Microbiology 41, 9–16.CrossRefGoogle ScholarPubMed
Lattimer, G. L., Luther, V., Rhodes, III., Salventi, J. F. & Cepil, B. R. (1980). Isolation of Legionella pneumophila from clinical specimens: salutary effects of lung tissue dilution. American review of respiratory diseases 122, 101–105.Google ScholarPubMed
Lattimer, G. L. & Ormsbee, R. A. (1981). Legionnaires11 Disease, p. 13. New York, Basel: Marcel Dekker.Google Scholar
M. R. C.(1974). The Accredition and Recognition Schemes for Supplies of Laboratory Animals. Medical Research Council Laboratory Animals Centre, Manual Series No. 1. Carshalton, Surrey.Google Scholar
Orrison, L. H., Cherry, W. B. & Milan, D. (1981). Isolation of Legionella pneumophila from cooling tower water by filtration. Applied Environmental Microbiology 14, 1902–1905.Google Scholar
Town, J. O'H., Swann, R. A. & Bartlett, C. L. R. (1981). Isolation of Legionella pnemnophila from water systems: methods and preliminary results. British Medical Journal 282, 515–517.Google Scholar
Wadowsky, R. M. & Yee, R. B. (1981). Glycine containing selective medium for isolation of Legionellacae from environmental water supplies. Journal of Applied and Environmental Microbiology 14, 768–772.CrossRefGoogle Scholar