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Isolation of a non-haemadsorbing, non-cytopathic strain of African swine fever virus in Madagascar

Published online by Cambridge University Press:  16 July 2001

M. GONZAGUE ,
F. ROGER ,
A. BASTOS ,
C. BURGER ,
T. RANDRIAMPARANY ,
S. SMONDACK  and
C. CRUCIERE
M. GONZAGUE
Affiliation:
AFSSA-Lerpaz, 22, rue Pierre Curie, BP67, 94703 Maisons-Alfort Cedex, France
F. ROGER
Affiliation:
CIRAD-EMVT, TA 30/G, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France
A. BASTOS
Affiliation:
ARC-Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort 0110, South Africa Department of Zoology & Entomology, University of Pretoria, Pretoria 0002, South Africa
C. BURGER
Affiliation:
AFSSA-Lerpaz, 22, rue Pierre Curie, BP67, 94703 Maisons-Alfort Cedex, France
T. RANDRIAMPARANY
Affiliation:
Institut Pasteur de Madagascar, BP 1274, Antananarivo 101, Madagascar
S. SMONDACK
Affiliation:
AFSSA-Lerpaz, 22, rue Pierre Curie, BP67, 94703 Maisons-Alfort Cedex, France
C. CRUCIERE
Affiliation:
AFSSA-Lerpaz, 22, rue Pierre Curie, BP67, 94703 Maisons-Alfort Cedex, France
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Abstract

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African swine fever (ASF) suspected clinically in Madagascar (1998–9) was confirmed by polymerase chain reaction (PCR) and nucleotide sequencing, following virus isolation. No haemadsorption or cytopathic effect could be detected following leukocyte inoculation, but viral growth in cells was confirmed by PCR. Detection of ASF virus genome was carried out by amplification of a highly conserved region coding for the p72 protein. Nucleotide sequencing of the amplicon revealed 99·2% nucleotide identity between the recent Malagasy strains and a virus recovered from the 1994 outbreak in Mozambique (SPEC265). A serological survey performed on 449 sera, revealed that only 5·3% of the sera taken from pigs between 1998 and 1999 were positive.

Type
Research Article
Information
Epidemiology & Infection , Volume 126 , Issue 3 , June 2001 , pp. 453 - 459
Copyright
© 2001 Cambridge University Press