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Investigation into rabbit infusion media for the growth of Mycoplasma gallisepticum antigens for inoculation into rabbits

Published online by Cambridge University Press:  15 May 2009

Janet M. Bradbury  and
F. T. W. Jordan
Janet M. Bradbury
Affiliation:
Poultry Research Section, University of Liverpool Veterinary Field Station ‘Leahurst’, Neston, Wirral, Cheshire
F. T. W. Jordan
Affiliation:
Poultry Research Section, University of Liverpool Veterinary Field Station ‘Leahurst’, Neston, Wirral, Cheshire
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Summary

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Serum cholesterol in rabbits was elevated, by intravenous inoculation of 20 % Tween 80, to amounts thought to have been sufficient to support growth of Mycoplasma gallisepticum when 20 % of such serum was added to rabbit infusion broth.

However, better growth of the organism was obtained with a supplement of 5 % rabbit serum and commercial cholesterol. Investigation showed that normal rabbit serum may be inhibitory, which would explain these findings. The commercial cholesterol was not antigenic.

Type
Research Article
Information
Journal of Hygiene , Volume 69 , Issue 1 , March 1971 , pp. 73 - 82
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Argaman, M. & Razin, S. (1965). Cholesterol and cholesterol esters in. mycoplasma. Journal of General Microbiology 38, 153.CrossRefGoogle ScholarPubMed
Butler, M. & Knight, B. C. J. G. (1960). The survival of washed suspensions of mycoplasma. Journal of General Microbiology 22, 470.CrossRefGoogle ScholarPubMed
Davies, G. (1969). Observations on the growth-inhibiting properties of some antisera to Mycoplasma mycoides. Journal of Comparative Pathology 79, 293.CrossRefGoogle Scholar
Edward, D. G. FF. (1954). Pleuropneumonia group of organisms: a review, together with some new observations. Journal of General Microbiology 10, 27.CrossRefGoogle ScholarPubMed
Edward, D. G. FF. & Fitzgerald, W. A. (1951 a). The isolation of organisms of the pleuropneumonia group from dogs. Journal of General Microbiology 5, 566.Google Scholar
Edward, D. G. FF. & Fitzgerald, W. A. (1951 b). Cholesterol in the growth of organisms of the pleuropneumonia group. Journal of General Microbiology 5, 576.CrossRefGoogle ScholarPubMed
Edward, D. G. FF. & Freundt, E. A. (1956).The classification and nomenclature of organisms of the pleuropneumonia group. Journal of General Microbiology 14, 197.CrossRefGoogle ScholarPubMed
Eng, J. (1967). Studies on the complement fixation test with Mycoplasma pneumoniae antigen. 1. Production of antigen. Acta pathologica et microbiologica scandinavica 71, 79.Google Scholar
Fernald, G. W., Clyde, W. A. Jr., & Denny, F. W. (1967). Factors influencing growth inhibition of Mycoplasma pneumoniae by immune sera. Proceedings of the Society for Experimental Biology and Medicine 126, 161.CrossRefGoogle ScholarPubMed
Friedrich, H. (1970). Zur Anwendung des Stoffwechselhemmtests (M1 test) bei Mycoplasma gallisepticum. Deutsche Tierärztliche Wochenschrift 77, 12.Google Scholar
Jordan, F. T. W. & Kulasegaram, P. (1968). Non-specific antibodies in chickens inoculated intratracheally with Mycoplasma gallisepticum. Journal of Comparative Pathology 78, 407.Google Scholar
Kabat, E. A. & Mayer, M. M. (1961). Experimental Immunochemistry, 2nd ed., p. 271. Springfield, Illinois: Charles C. Thomas.Google Scholar
Kellner, A., Correll, J. W. & Ladd, A. T. (1951). Sustained hyperlipemia induced in rabbits by means of intravenously injected surface-active agents. Journal of Experimental Medicine 93, 373.CrossRefGoogle ScholarPubMed
Kenny, G. E. & Grayston, J. T. (1965). Eaton pleuropneumonia-like organism (Mycoplasma pneumoniae) complement-fixing antigen. Extraction with organic solvents. Journal of Immunology 95, 19.CrossRefGoogle Scholar
Kulasegaram, P. (1967). Serological studies on M. gallisepticum. Ph.D. thesis, University of Liverpool.Google Scholar
Meynell, G. G. & Meynell, E. (1965). Theory and Practice in Experimental Bacteriology, p. 204. Cambridge University Press.Google Scholar
Moran, P. A. P. (1954 a). The dilution assay of viruses. I. Journal of Hygiene 52, 189.CrossRefGoogle Scholar
Moran, P. A. P. (1954 b). The dilution assay of viruses. II. Journal of Hygiene 52, 444.CrossRefGoogle Scholar
Moran, P. A. P. (1958). Another test for heterogeneity of host resistance in dilution assays. Journal of Hygiene 56, 319.Google Scholar
Nutor, B. L. (1969). The isolation of avian mycoplasma from field material and the serological response of turkeys to M. gallisepticum. M.V.Sc. thesis, University of Liverpool.Google Scholar
Rodwell, A. W. (1963). The steroid growth requirement of Mycoplasma mycoides. Journal of General Microbiology 32, 91.Google Scholar
Rodwell, A. W. & Abbot, A. (1961). The function of glycerol, cholesterol and long-chain fatty acids in the nutrition of Mycoplasma mycoides. Journal of General Microbiology 25, 201.CrossRefGoogle ScholarPubMed
Sammons, S. S., Gardner, E. Jr. & Dienst, R. B. (1968). A medium for growth and immunological studies on mycoplasma. Bacteriological Proceedings, p. 68.Google Scholar
Searcy, R. L. & Bergquist, L. M. (1960). A new colour reaction for the quantitation of serum cholesterol. Clinica chimica Acta 5, 192.Google Scholar
Smith, P. F. (1960). Nutritional requirements of PPLO and their relation to metabolic function. Annals of the New York Academy of Sciences 79, 508.Google Scholar
Smith, P. F., Lecce, J. G. & Lynn, R. J. (1954). A lipoprotein as a growth factor for certain pleuropneumonia-like organisms. Journal of Bacteriology 68, 627.CrossRefGoogle Scholar
Smith, S. C., Dunlop, W. R. & Strout, R. G. (1966). Effect of culture medium on antigenic structure of mycoplasma. Avian Diseases 10, 173.CrossRefGoogle ScholarPubMed
Taylor, J. (1962). The estimation of numbers of bacteria by tenfold dilution series. Journal of Applied Bacteriology 25, 54.Google Scholar
Taylor-Robinson, D., Purcell, R. H., Wong, D. C. & Chanock, R. M. (1966). A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. Journal of Hygiene 64, 91.CrossRefGoogle ScholarPubMed
Taylor-Robinson, D., Somerson, N. L., Turner, H. C. & Chanock, R. M. (1963). Serological relationships among human mycoplasmas as shown by complement-fixation and gel diffusion. Journal of Bacteriology 85, 1261.Google Scholar