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The inhibition of growth of vaccinia and cowpox viruses in RK 13 cells

Published online by Cambridge University Press:  15 May 2009

Derrick Baxby  and
C. J. M. Rondle
Derrick Baxby
Affiliation:
Department of Bacteriology, Liverpool University, Liverpool 3, England
C. J. M. Rondle
Affiliation:
Department of Bacteriology, Liverpool University, Liverpool 3, England
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The growth of vaccinia and cowpox in RK13 cells was studied by measurement of infective virus production, gel diffusion and haemagglutination. The effects of virus growth inhibitors on the normal course of infection were then followed. Although the results with the two viruses were essentially similar some differences were detected between them. Inhibitors of DNA synthesis permitted production of many virus-specific soluble antigens. Compounds which inhibit DNA function and compounds that affected energy-yielding reactions had more dramatic effects. The different results obtained suggested that the synthesis of virus-specific materials was sequential and a possible part of the sequence is suggested.

Type
Original Articles
Information
Journal of Hygiene , Volume 66 , Issue 2 , June 1968 , pp. 191 - 205
Copyright
Copyright © Cambridge University Press 1968

References

Appleyard, G., Hume, V. B. M. & Westwood, J. C. N. (1965). The effect of thiosemicarbazones on the growth of rabbitpox virus in tissue cultures. Ann. N.Y. Acad. Sci. 130, 92.CrossRefGoogle Scholar
Appleyard, G. & Westwood, J. C. N. (1964). The growth of rabbitpox virus in tissue culture. J. gen. Microbiol. 37, 391.CrossRefGoogle ScholarPubMed
Appleyard, G., Westwood, J. C. N. & Zwartouw, H. T. (1962). The toxic effect of rabbitpox virus. Virology 18, 159.CrossRefGoogle ScholarPubMed
Bauer, D. J. (1961). The zero effect dose (E0) as an absolute numerical index of antiviral chemotherapeutic activity in the poxvirus group. Br. J. exp. Path. 42, 201.Google Scholar
Baxby, D. (1967). The growth and titration of vaccinia and cowpox viruses, and production of their soluble antigens in tissue culture. Ph.D. thesis, Liverpool University.Google Scholar
Baxby, D. & Rondle, C. J. M. (1965). Plaque formation by vaccinia virus in tissue cultures inhibited by 5-fluorodeoxyuridine. J. gen. Microbiol. 38, 423.Google Scholar
Baxby, D. & Rondle, C. J. M. (1967). The relative sensitivity of chick and rabbit tissues for the titration of vaccinia and cowpox viruses. Arch. ges. Virusforsch. 20, 263.Google Scholar
Beale, A. J., Christofinis, G. C. & Furminger, I. G. S. (1963). Rabbit cells susceptible to rubella. Lancet ii, 640.CrossRefGoogle Scholar
Bubel, H. C. & Wolff, D. A. (1965). Proflavine inhibition of vaccinia virus synthesis. J. Bact. 89, 977.Google Scholar
Easterbrook, K. B. (1961). Analysis of the early stages of vaccinia virus infection in KB cells using sodium azide. Virology 15, 417.CrossRefGoogle ScholarPubMed
Easterbrook, K. B. (1962). Interference with the maturation of vaccinia virus by isatin-β-thiosemicarbazone. Virology 27, 245.CrossRefGoogle Scholar
Easterbrook, K. B. & Davern, C. I. (1963). The effect of 5-bromodeoxyuridine on the multiplication of vaccinia virus. Virology 17, 509.CrossRefGoogle Scholar
Fenner, F. (1958). The biological characteristics of several strains of vaccinia, cowpox and rabbitpox viruses. Virology 5, 502.Google Scholar
Fujio, Y. (1963). Effects of actinomycin on growth and haemagglutinin production of vaccinia virus. Biken J. 6, 197.Google Scholar
Gispen, R. (1955). Analysis of poxvirus antigens by means of double-diffusion. A method for direct serological differentiation of cowpox. J. Immun. 74, 134.CrossRefGoogle ScholarPubMed
Hume, V. B. M., Westwood, J. C. N. & Appleyard, G. (1965). The antiviral activity of rutilantin A. J. gen. Microbiol. 38, 143.Google Scholar
Joklik, W. K. (1966). The poxviruses. Bact. Rev. 30, 33.CrossRefGoogle ScholarPubMed
Keir, H. M., Subak-Sharpe, H., Sheddon, W. I. H., Watson, D. H. & Wildy, P. (1966). Immunological evidence for a specific DNA polymerase produced after infection with herpes simplex virus. Virology 30, 155.Google Scholar
Kersten, H. (1962). Action of mitomycin C on nucleic acid metabolism in tumour and bacterial cells. Biochim. biophys. Acta 55, 558.Google Scholar
Kersten, H., Kersten, W., Leopold, G. & Schniedeb, B. (1964). Effect of mitomycin C on DNA-ase and RNA in E. coli. Biochim. biophys. Acta 80, 521.Google Scholar
Loh, P. C. & Payne, F. E. (1965). Effects of 5-fluorodeoxyuridine on the synthesis of vaccinia virus. Virology 25, 575.Google Scholar
Marquardt, J., Holme, E. & Lycke, E. (1965). Immunoprecipitating factors of vaccinia virus. Virology 27, 170.Google Scholar
McCarthy, K. & Dumbell, K. R. (1961). Chorioallantoic inoculation of eggs, an improved method. Virology 14, 488.CrossRefGoogle ScholarPubMed
McCarthy, K. & Helbert, D. (1960). Comparison of haemagglutinins of variola, alastrim, vaccinia, cowpox and ectromelia. J. Path. Bact. 79, 416.CrossRefGoogle Scholar
Oda, M. (1963). Vaccinia virus-HeLa cell interaction. II. The effect of mitomycin C on the production of infective virus, complement-fixing antigen and haemagglutinin. Virology 21, 533.Google Scholar
Pricer, W. E. Jr. & Weissbach, A. (1964). The effect of lysogenic induction with mitomycin C on the DNA and DNA polymerase of E. coli K 12 λ. Biochem. biophys. Res. Commun. 14, 91.CrossRefGoogle Scholar
Rondle, C. J. M. & Dumbell, K. R. (1962). Antigens of cowpox virus. J. Hyg., Comb. 60, 41.Google Scholar
Rosenkranz, H. S., Rose, H. M., Morgan, C. & Hsu, K. C. (1966). The effect of hyd-roxyurea on virus development. II. Vaccinia virus. Virology 28, 510.Google Scholar
Salzman, N. P., Shatkin, A. J. & Sebring, E. D. (1963). Viral protein and DNA synthesis in vaccinia virus infected HeLa cells. Virology 19, 542.CrossRefGoogle Scholar
Smadel, J. E. & Shedlovsky, T. (1942). Antigens of vaccinia. Ann. N.Y. Acad. Sci. 43, 35.CrossRefGoogle Scholar
Thompson, R. L. (1947). The effect of metabolites, metabolite antagonists and enzyme inhibitors on the growth of the vaccinia virus in Maitland-type tissue cultures. J. Immun. 55, 345.Google Scholar
Wilcox, W. C. & Ginsberg, H. S. (1962). Effect of proflavine on the synthesis of adenovirus 5 and associated soluble antigens. J. Bact. 84, 526.CrossRefGoogle ScholarPubMed
Williamson, J. D. (1963). Vaccinia soluble antigen. Ph.D. thesis, Liverpool University.Google Scholar
Williamson, J. D. & Rondle, C. J. M. (1964). A virus-specific hapten from vaccinia infected sheep dermis. Biochem. J. 90, 13 P.Google Scholar