Hostname: page-component-788cddb947-2s2w2 Total loading time: 0 Render date: 2024-10-14T21:10:04.888Z Has data issue: false hasContentIssue false
  • English
  • Français

Further studies on the growth of rubella virus in human embryonic organ cultures: preliminary observations on interferon production in these cultures

Published online by Cambridge University Press:  15 May 2009

Jennifer M. Best ,
J. E. Banatvala  and
M. E. Smith
Jennifer M. Best
Affiliation:
Department of Clinical Virology, St Thomas's Hospital and Medical School, London, S.E.1
J. E. Banatvala
Affiliation:
Department of Clinical Virology, St Thomas's Hospital and Medical School, London, S.E.1
M. E. Smith
Affiliation:
Department of Ophthalmology, Chelmsford and Essex Hospital
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Organ cultures prepared from 15 different organs obtained from 43 fetuses were consistently found to support the growth of rubella virus, irrespective of the gestational age of the fetus or the strain of rubella virus inoculated. Although rubella virus replicated in fetal lenses, adult lenses did not support the growth of rubella virus. Organs obtained from four fetuses between 8 and 17 weeks gestational age produced similar titres of an inhibitor which had the characteristics of interferon. The use of Trowell T8 medium and incubation in a mixture of 5% CO2 in oxygen provided the most suitable conditions for the maintenance of most organ cultures. Under these circumstances it was possible to obtain adequate histological preparations from these organs, but light microscopy studies revealed no significant differences in sections of rubella inoculated and control organ cultures.

Type
Research Article
Information
Journal of Hygiene , Volume 69 , Issue 2 , June 1971 , pp. 223 - 232
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Almeida, J. D. & Tyrrell, D. A. J. (1967). The morphology of three previously uncharacterized human respiratory viruses that grow in organ culture. Journal of General Virology 1, 175.CrossRefGoogle ScholarPubMed
Best, J. M. & Banatvala, J. E. (1967). A comparison of RK-13, vervet monkey kidney and patas monkey kidney cell cultures for the isolation of rubella virus. Journal of Hygiene 65, 263.CrossRefGoogle ScholarPubMed
Best, J. M., Banatvala, J. E. & Moore, B. M. (1968). Growth of rubella virus in human embryonic organ cultures. Journal of Hygiene 66, 407.CrossRefGoogle ScholarPubMed
Cantell, K., Strander, H., Saxen, L. & Meyer, B. (1968). Interferon response of human leukocytes during intrauterine and postnatal life. Journal of Immunology 100, 1304.CrossRefGoogle ScholarPubMed
Hardy, J. B., McCracken, G. H., Gilkeson, M. R. & Sever, J. L. (1969). Adverse fetal outcome following maternal rubella after the first trimester of pregnancy. Journal of the American Medical Association 207, 2414.CrossRefGoogle ScholarPubMed
Heineberg, F., Gold, E. & Robbins, F. C. (1964). Differences in interferon content in tissues of mice of various ages infected with coxsackie B1 virus. Proceedings of the Society for Experimental Biology and Medicine 115, 947.CrossRefGoogle ScholarPubMed
Hoorn, B. & Tyrrell, D. A. J. (1965). The growth of certain ‘newer’ respiratory viruses in organ cultures. British Journal of Experimental Pathology 46, 109.Google ScholarPubMed
Isaacs, A. & Baron, S. (1960). Antiviral action of interferon in embryonic cells. Lancet, ii, 946.CrossRefGoogle Scholar
Kistler, G. S., Best, J. M., Banatvala, J. E. & Tönbury, G. (1967). Elektronenmikroskopische Untersuchungen an rötelninfizierten menschlichen Organkulturen. Schweizerische Medizinische Wochenschrift 97, 1377.Google Scholar
Lucas, D. R. & Trowell, O. A. (1958). In vitro culture of the eye and the retina of the mouse and rat. Journal of Embryology and Experimental Morphology 6, 178.Google ScholarPubMed
McCarthy, K. & Taylor-Robinson, C. H. (1965). Growth and cytopathic effects of rubella virus in primary rabbit tissue culture. Archiv für die gesamte Virusforschung 16, 415.CrossRefGoogle ScholarPubMed
Mims, C. A. (1968). Pathogenesis of viral infections of the fetus. In Progress in Medical Virology, vol. 10, p. 194. Ed. Melnick, J. L.. Karger. Basel. New York.Google Scholar
Rawls, W. E. (1968). Congenital rubella: the significance of virus persistance. In Progress in Medical Virology, vol. 10, p. 238. Ed. Melnick, J. L.. Karger. Basel. New York.Google Scholar
Rawls, W. E., Desmyter, J. & Melnick, J. L. (1968). Serologic diagnosis and fetal involvement in maternal rubella. Journal of the American Medical Association 203, 627.CrossRefGoogle ScholarPubMed
Sawicki, L. (1961). Influence of age of mice on the recovery from experimental Sendai virus infection. Nature, London 192, 1258.CrossRefGoogle Scholar
Siewers, C. M. F., John, C. E. & Medearis, D. N. (1970). Sensitivity of human cell strains to interferon. Proceedings of the Society for Experimental Biology and Medicine 133, 1178.CrossRefGoogle ScholarPubMed
Smorodintsev, A. A. (1968). The production and effect of interferon in organ cultures of calf trachea. British Journal of Experimental Pathology 49, 511.Google ScholarPubMed
Thompson, K. M. & Tobin, J. O'H. (1970). Isolation of rubella virus from abortion material. British Medical Journal 1, 264.CrossRefGoogle Scholar
Trowell, O. A. (1959). The culture of mature organs in a synthetic medium. Experimental Cell Research 16, 118.CrossRefGoogle Scholar
Tyrrell, D. A. J. & Bynoe, M. L. (1965). Cultivation of a novel type of common-cold virus in organ cultures. British Medical Journal 1, 1467.CrossRefGoogle ScholarPubMed