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Fluid and solid media for isolation of Brucella abortus

Published online by Cambridge University Press:  15 May 2009

J. Brodie  and
G. P. Sinton
J. Brodie
Affiliation:
Scottish Brucella Reference Laboratory, City Hospital, Aberdeen
G. P. Sinton
Affiliation:
Scottish Brucella Reference Laboratory, City Hospital, Aberdeen
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Summary

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The use of a fluid-enrichment technique increased by 10–16% the recovery rate of Brucella abortus from milks of herds positive to a milk ring test. Three solid media for direct cultural techniques were also tested on the same milks. Combinations of these techniques produced a recovery rate of ca. 88% from MRT-positive-herd milks using a mixture of the centrifuged deposit and cream layer as the inoculum as against a recovery rate of ca. 75% using the MRT cream layer as inoculum. B. abortus biotypes 1–5 and 9 were isolated and growth of all the biotypes was supported by all media used.

Type
Research Article
Information
Journal of Hygiene , Volume 74 , Issue 3 , June 1975 , pp. 359 - 367
Copyright
Copyright © Cambridge University Press 1975

References

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