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Experimental infection of badgers (Meles meles) with Mycobacterium bovis

Published online by Cambridge University Press:  19 October 2009

D. G. Pritchard
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey, U.K.
Fiona A. Stuart
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey, U.K.
Jacky I. Brewer
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey, U.K.
K. H. Mahmood
Affiliation:
School of Pathology, Middlesex Hospital Medical School, Riding House Street, London W1
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The intradermal inoculation of four badgers with small numbers of Mycobacterium bovis resulted in localized lesions with ulceration which slowly healed by 5 months after inoculation. Lesions of generalized tuberculosis were seen in three badgers, one of which died at 17 months post-inoculation and in the remaining two killed 22 months post-inoculation. In the fourth badger lesions were confined to the draining lymph node of the inoculation site but M. bovis was isolated from the liver. Monthly clinical sampling of faeces, urine, tracheal aspirate and inoculation site exudates detected only the excretion of M. bovis from the inoculation site of one badger. There were marked seasonal variations in body weight but significant weight loss was observed during the second year in all four badgers, particularly prior to death. Four badgers inoculated intratracheally with a similar inoculum of M. bovis and another two control badgers showed no evidence of infection with M. bovis.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1987

References

REFERENCES

Anon (1984). Bovine Tuberculosis in Badgers. Eighth Report by the Ministry of Agriculture, Fisheries and Food.Google Scholar
Barrow, P. & Gallagher, J. (1981). Aspects of the epidemiology of bovine tuberculosis in badgers and cattle. I. The prevalance of infection in two wild animal populations in South-West England. Journal of Hygiene 86, 237245.Google Scholar
Beutler, B., Greenwald, D., Hulmes, J. D., Chang, M., Pan, Y. C. E., Mathison, J., Ulevitch, R. & Cerami, A. (1985). Identity of tumour necrosis factor and the macrophage secreted factor cachectin. Nature 316, 552554.CrossRefGoogle ScholarPubMed
Brown, I. N. (1983). Animal models and immune mechanisms in mycobacterial infections. In Biology of Mycobacteria, vol. 2 (ed. Ratledge, C. and Stanford, J. L.), pp. 173234. London: Academic Press.Google Scholar
Cheeseman, C. L. & Harris, S. (1982). Methods of marking badgers (Meles meles). Journal of Zoology, London 197, 289292.Google Scholar
Cheeseman, C. L., Little, T. W. A., Mallinson, P. J., Rees, W. A. & Wilesmith, J. W. (1985). The progression of bovine tuberculosis infection in a population of Meles meles in South-West England. Acta Zoologica Fennica 173, 197199.Google Scholar
Corper, H. J. & Uyei, N. (1930). Oxalic acid as a reagent for isolating tubercle bacilli. Journal of Laboratory and Clinical Medicine 15, 348369.Google Scholar
Dinarello, C. A. (1984). In Advances in Inflammation Research (ed. Weissmann, G.), 8th ed. pp. 203225. New York: Raven.Google Scholar
Fox, M. W. (1968). Abnormal behaviour in zoo animals. In Abnormal Behaviour in Animals, p. 498. Philadelphia: W. B. Saunders.Google Scholar
Francis, J. (1958). In Tuberculosis in Animals and Man, pp. 67, 16–21, 301. London: Cassell.Google Scholar
Gallagher, J. & Horwill, D. M. (1977). A selective oleic acid albumin agar medium for the cultivation of Mycobacterium bovis. Journal of Hygiene 79, 155160.CrossRefGoogle ScholarPubMed
Gallagher, J., Muirhead, R. H. & Burn, K. J. (1976). Tuberculosis in wild badgers in Gloucestershire: pathology. Veterinary Record 105, 546551.Google Scholar
Higgins, D. A., Kung, I. T. M. & Or, R. S. B. (1985). Environmental silica in badger lungs: a possible association with susceptibility to Mycobacterium bovis infection. Infection and Immunity 48, 251256.CrossRefGoogle ScholarPubMed
Huitema, H. (1970). The tuberculin test in cattle and other animals. First International Seminar on Bovine Tuberculosis for the Americas. Santiago, Chile, pp. 171188. WHO, Washington, U.S.A., 1972.Google Scholar
Inman, P. M., Beck, A., Brown, A. E. & Stanford, J. L. (1969). Outbreak of injection abscesses due to Mycobacterium abscessus. Archives of Dermatology 100, 141147.CrossRefGoogle ScholarPubMed
Kruuk, H. & Parrish, T. (1983). Seasonal and local differences in the weight of European badgers (Meles meles) in relation to food supply. Zeitschrift für Saugetierlcunde 48, 4550.Google Scholar
Lepper, A. W. D. & Corner, L. A. (1983). Naturally occurring Mycobacterioses in animals. In Biology of the Mycobacteria, vol. 2 (ed. Ratledge, C. and Stanford, J. L.), pp. 270472. London: Academic Press.Google Scholar
Lesslie, I. W. (1959). A comparison of biological and some cultural methods for the primary isolation of Mycobacterium tuberculosis. Journal of Comparative Pathology 69, 110.CrossRefGoogle ScholarPubMed
Little, T. W. A., Swan, C., Thompson, H. W. & Wilesmith, J. W. (1982). Bovine tuberculosis in domestic and wild mammals in an area of Dorset. II. The badger population, its ecology and tuberculosis status. Journal of Hygiene 89, 211224.Google Scholar
Little, T. W. A., Naylor, P. F. & Wilesmith, J. W. (1982). Laboratory study of Mycobacterium bovis infection in badgers and calves. Veterinary Record 111, 550557.Google ScholarPubMed
Lurie, M. B. (1941). Heredity, constitution and tuberculosis – an experimental study. II. Inherited native resistance and artificial tuberculous infection. American Review of Tuberculosis 44, 6879 (Supplement).Google Scholar
Mackintosh, C. G., MacArthur, J. A., Little, T. W. A. & Stuart, P. (1976). The immobilization of the badger (Meles meles). British Veterinary Journal 132, 609614.CrossRefGoogle ScholarPubMed
Mahmood, K. H. (1985). The immunological unresponsiveness of badgers to mycobacteria. Ph.D. thesis. University of London.Google Scholar
Mahmood, K. H., Rook, G. A. W., Stanford, J. L., Stuart, Fiona A. & Pritchard, D. G. (1987). The immunological consequence of challenge with bovine tubercle bacilli in badgers (Meles meles). Epidemiology and Infection 98, 155163.CrossRefGoogle ScholarPubMed
Marks, J. (1976). A system for the examination of tubercle bacilli and other mycobacteria. Tubercle 57, 207225.Google Scholar
Miles, A. A., Misra, S. S. & Irwin, J. O. (1938). The estimation of the bactericidal power of the blood. Journal of Hygiene 38, 732749.Google Scholar
Muirhead, R. H., Gallagher, J. & Burn, K. J. (1974). Tuberculosis in wild badgers in Gloucestershire: epidemiology. Veterinary Record 95, 552555.CrossRefGoogle Scholar
Neal, E. (1975). The Badger, pp. 34, 108–110. London: Collins.Google Scholar
Pritchard, D. G., Stuart, F. A.Wilesmith, J. W., Cheeseman, C. L., Bode, R. & Sayers, P. (1986). Tuberculosis in East Sussex. III. Comparison of the post mortem and clinical methods for the diagnosis of tuberculosis in badgers. Journal of Hygiene 97, 2736.Google Scholar
Vestal, A. L. (1975). Procedures for the Isolation and Identification of Mycobacteria. U.S. Department of Health, Education and Welfare Publication no. (CDC) 75–8230, p. 86.Google Scholar
Wells, W. F., Ratcliffe, H. L. & Crumb, C. (1948). On the mechanics of droplet nuclei infection. II. Quantitative experimental air-borne tuberculosis in rabbits. American Journal of Hygiene AT, 1128.Google Scholar
White, E. G. and Minett, F. C. (1941). Pathogenesis of tuberculosis in the calf. British Journal of Tuberculosis 35, 6987.Google Scholar
Wilesmith, J. W. (1983). Epidemiological features of bovine tuberculosis in cattle herds in Great Britain. Journal of Hygiene 90, 159176.CrossRefGoogle ScholarPubMed