Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-12-02T19:29:50.568Z Has data issue: false hasContentIssue false
  • English
  • Français

Epidemiology of Salmonella typhimurium: ribosomal DNA analysis of strains from human and animal sources

Published online by Cambridge University Press:  15 May 2009

A. Nastasi ,
C. Mammina  and
M. R. Villafrate
A. Nastasi
Affiliation:
Department of Hygiene & Microbiology ‘G. D’Alessandro’, Center for Enterobacteriaceae of Southern Italy, University of Palermo, via del Vespro 133, 90127 Palermo, Italy
C. Mammina
Affiliation:
Department of Hygiene & Microbiology ‘G. D’Alessandro’, Center for Enterobacteriaceae of Southern Italy, University of Palermo, via del Vespro 133, 90127 Palermo, Italy
M. R. Villafrate
Affiliation:
Department of Hygiene & Microbiology ‘G. D’Alessandro’, Center for Enterobacteriaceae of Southern Italy, University of Palermo, via del Vespro 133, 90127 Palermo, Italy
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Salmonella typhimurium is the most frequently identified serovar of Salmonella in Italy. This serovar is characterized by the widespread dissemination among human and non-human sources of phenotypically and genetically well-differentiated clones.

In this study 457 strains of S. typhimurium isolated in Italy in the years 1982–91 from human and animal sources were submitted to characterization by the rDNA fingerprinting technique. Application of this typing method, after digestion of chromosomal DNA with HincII endonuclease, confirmed the greatest genetic differentiation of clones of S. typhimurium, allowing reliable identification of 45 rDNA patterns linked into 9 major clusters. rDNA pattern clusters or ribotypes specific to man were not recognized, whereas some rDNA patterns were characteristically related to ducks, pigeons and pet birds. The ribotyping results for isolates from animal hosts suggest that pig and cattle are the main source of human infection.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 3 , June 1993 , pp. 553 - 565
Copyright
Copyright © Cambridge University Press 1993

References

REFERENCES

1.Barker, R, Old, DC, Sharp, JCM. Phage type/biotype groups of Salmonella typhimurium in Scotland 1974–6: variation during spread of epidemic clones. J Hyg 1980; 84: 115–25.Google ScholarPubMed
2.Brunner, F, Margadant, A, Peduzzi, R. The plasmid pattern as an epidemiological tool for Salmonella typhimurium epidemics: comparison with the Iysotype. J Infect Dis 1983: 148: 7–11.CrossRefGoogle Scholar
3.Forster, AC, McInnes, JL, Skingle, DC, Symons, RH. Non-radioactive hybridization probes prepared by chemical labelling of DNA and RNA with a novel reagent, photobiotin. Nucl Acids Res 1985; 3: 745–61.CrossRefGoogle Scholar
4.Anderson, ES, Ward, LR, DeSaxe, MJ, Old, DC, Barker, R. Duguid, JP. Correlation of phage type. biotype and source in strains of Salmonella typhimurium. J Hyg 1978; 81: 203–17.CrossRefGoogle ScholarPubMed
5.Van Leuween, WJ, Pruys, D, Guinée, PAM. Phage typing of S. typhimurium in the Netherlands. 2. Frequency distribution of S. typhimurium phage types in the Netherlands during 1971 and 1972. Zbl Bakt Hyg 1974; I Abt Orig A 226: 201–6.Google Scholar
6.Van Leuween, WJ, Guinée, PAM. Frequency distribution of S. typhimurium phage types in various countries. Zbl Bakt Hyg 1975; 1 Abt Orig A 230: 320–35.Google Scholar
7.Grimont, F, Grimont, PAD. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur/Microbiol 1986; 137 b: 165–75.CrossRefGoogle Scholar
8.Koblavi, S, Grimont, F, Grimont, PAD. Clonal diversity of Vibrio cholerae O1 evidenced by rRNA gene restriction patterns. Res Microbiol 1990; 141: 645–57.CrossRefGoogle ScholarPubMed
9.Stull, TL, LiPuma, JJ, Edlind, TD. A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis 1988; 157: 280–6.CrossRefGoogle ScholarPubMed
10.Thomson-Carter, FM, Carter, PE, Pennington, TH. Differentiation of staphylococcal species and strains by ribosomal RNA gene restriction patterns. J Gen Microbiol 1989; 135: 2093–7.Google ScholarPubMed
11.Holmberg, SD, Wachsmuth, IK, Hickmann-Brenner, FW, Cohen, ML. Comparison of plasmid profile analysis, phage typing, and antimicrobial sensitivity testing in characterizing Salmonella typhimurium isolates from outbreaks. J Clin Microbiol 1984; 19: 100–4.CrossRefGoogle ScholarPubMed
12.Nastasi, A, Mammina, C, Villafrate, MR. rDNA fingerprinting as a tool in epidemiological analysis of Salmonella typhi infections. Epidemiol Infect 1991; 107: 565–76.CrossRefGoogle ScholarPubMed
13.Wauters, G. Carriage of Y. enterocolitica serotype 3 by pigs as a source of human infection. Contr Microbiol Immunol 1979; 5: 249–52.Google ScholarPubMed