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Enrichment procedures for the isolation of Salmonella, Arizona, Edwardsiella and Shigella from faeces

Published online by Cambridge University Press:  15 May 2009

J. B. Iveson
J. B. Iveson
Affiliation:
Salmonella Reference Laboratory, Box F. 312, State Health Laboratory Service, Perth, Western Australia
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Summary

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Strontium selenite A broth incubated at 37° C., and strontium chloride B broth incubated at 43° C., have been found an effective combination for the isolation of Salmonella, Arizona and Edwardsiella from human faeces.

Direct plating on deoxycholate citrate agar was superior to enrichment methods for the isolation of Shigella species.

Strontium selenite A broth was suitable for the isolation of Salmonella and Arizona bacteria at both 37° and 43° C.

Strontium chloride B broth incubated at 43° C. was best for the isolation of Salmonella and Edwardsiella.

Modified bismuth sulphite agar, although superior to D.c. agar for the growth of Salmonella and Arizona bacteria, was unsuitable for Edwardsiella and Shigella species.

A considerable difference was observed in the distribution of Salmonella, Arizona and Edwardsiella serotypes isolated from humans in remote areas, when compared with isolations from more densely populated urban and agricultural centres.

Epidemiological and zoogeographical aspects of host–parasite associations between humans, animals and pathogenic Enterobacteriaceae in Western Australia are discussed.

Type
Research Article
Information
Journal of Hygiene , Volume 71 , Issue 2 , June 1973 , pp. 349 - 361
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

Beijerinck, M. W. (1901). Enrichment culture studies with urea bacteria. Zentralblatt für Bacteriologie, Part II, 7, 33.Google Scholar
Bhat, P., Shanthakumari, S. & Myers, M. (1969). The Arizona group of organisms in the aetiology of childhood diarrhoea. Indian Journal of Medical Research 57, 1240.Google Scholar
Chau, P. Y. & Huang, C. T. (1971). Carriage rate of Salmonella serotypes in hospital patients and comparison of enrichment media for their isolation. Tropical Medicine 13, 94.Google Scholar
Cherubin, C. E. & Winter, J. (1970). Salmonella infection with multiple serotypes. American Journal of the Medical Sciences 260, 34.Google ScholarPubMed
Collard, P. & Unwin, M. (1958). A trial of Rappaport's medium. Journal of Clinical Pathology 11, 426.Google Scholar
Cook, G. T., Frisby, B. R. & Jebb, W. H. H. (1951). The routine use of selective and enrichment media for the isolation of salmonellae. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 10, 89.Google ScholarPubMed
Galton, M. M. & Quan, M. S. (1944). Salmonella isolated in Florida during 1943 with the combined enrichment method of Kauffmann. American Journal of Public Health 34, 1071.Google Scholar
Gray, J. D. A. (1931). New lithium selective and enrichment methods for the isolation of Salmonella organisms. Journal of Pathology and Bacteriology 34, 335.CrossRefGoogle Scholar
Harvey, R. W. S. & Price, T. H. (1962). Salmonella serotypes and Arizona paracolons isolated from Indian crushed bone. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 21, 54.Google Scholar
Harvey, R. W. S. & Price, T. H. (1968). Elevated temperature incubation of enrichment media for the isolation of Salmonella from heavily contaminated materials. Journal of Hygiene 66, 377.Google Scholar
Hooper, W. L. & Jenkins, H. R. (1965). An evaluation of Rappaport's magnesium chloride malachite green medium in the routine examination of faeces. Journal of Hygiene 63, 491.Google ScholarPubMed
Iveson, J. B. (1971). Strontium chloride B and E.E. enrichment broth media for the isolation of Edwardsiella, Salmonella and Arizona species from tiger snakes. Journal of Hygiene 69, 323.CrossRefGoogle ScholarPubMed
Iveson, J. B. & Kovacs, N. (1967). A comparative trial of Rappaport enrichment medium for the isolation of Salmonellae from faeces. Journal of Clinical Pathology 20, 290.Google Scholar
Iveson, J. B., Kovacs, N. & Laurie, W. (1964). An improved method for the isolation of Salmonellae from desiccated coconut. Journal of Clinical Pathology 17, 75.CrossRefGoogle ScholarPubMed
Iveson, J. B., Mackay-Scollay, E. M. & Bamford, V. W. (1969). Salmonella and Arizona in reptiles and man in Western Australia. Journal of Hygiene 67, 135.Google Scholar
Iveson, J. B. & Mackay-Scollay, E. M. (1969). Strontium chloride and strontium selenite enrichment broth media in the isolation of Salmonella. Journal of Hygiene 67, 457.CrossRefGoogle ScholarPubMed
Iveson, J. B. & Mackay-Scollay, E. M. (1972). An evaluation of strontium chloride, Rappaport and strontium selenite enrichment for the isolation of salmonellas from man, animals, meat products and abattoir effluents. Journal of Hygiene 70, 367.Google Scholar
Kaura, Y. K., Sharma, V. K., Martin, W. J., Singh, I. P. & Verma, P. S. (1971). Three new serotypes of Arizona (19:23:21, 16:29:25, 15:26:31). International Journal of Systematic Bacteriology 21, 83.Google Scholar
Miller, A. S. (1971). Salmonellosis in Botswana. I. Incidence in cattle. Journal of Hygiene 69, 491.Google Scholar
Rappaport, F., Konforti, N. & Navon, B. (1956). A new enrichment medium for certain salmonellae. Journal of Clinical Pathology 9, 261.CrossRefGoogle Scholar
Sachs, A. (1939). Difficulties associated with the bacteriological diagnosis of bacillary dysentery. Journal of the Royal Army Medical Corps 73, 235.Google Scholar
Sakasaki, R., Tamura, K., Prescott, L. M. & Bencic, Z. (1971). Bacteriological examination of diarrhoeal stools in Calcutta. Indian Journal of Medical Research 59, 1025.Google Scholar
Sharma, V. K., Kaura, Y. K. & Singh, I. P. (1970). Arizona infection in snakes, rats and man. Indian Journal of Medical Research 58, 409.Google Scholar
Zwart, P., Poelma, F. G. & Strik, W. J. (1970). The distribution of various types of Salmonellae and Arizonas in reptiles. Zentralblatt für Bakteriologie, Parasitenkunde, Infektion-skrankheiten und Hygiene (Abt. I, Orig) 213, 201.Google Scholar