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Electrophoretic characterisation of the outer membrane proteins of Yersinia pestis isolated in north-east Brazil

Published online by Cambridge University Press:  15 May 2009

F. G. C. Abath ,
A. M. P. Almeida  and
L. C. S. Ferreira
F. G. C. Abath
Affiliation:
Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE-59.739Brazil
A. M. P. Almeida
Affiliation:
Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE-59.739Brazil
L. C. S. Ferreira*
Affiliation:
Laboratório de Imunopatologia Keizo Asami and Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, PE-59.739Brazil
*
*Dr L. C. S. Ferreira. Laboratório de Imunopatologia Keizo Asami, Cidade Universitária, Recife, PE-50. 739. Brazil
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The outer membrane proteins of 38 Yersinia pestis isolates from all known plague foci of north-east Brazil were analysed by SDS-PAGE. Approximately 20 bands were consistently found in all strains analysed and 11 were selected for comparative studies. Although qualitative differences among the electrophoretic profiles of outer membrane proteins of wild Y. pestis isolates were not observed, quantitative alterations were clearly noted for most of these proteins. No particular quantitative alteration of the electrophoretic profile of outer membrane proteins could be associated with the period of isolation and geographic origin of the isolates. The 64 kDa outer membrane protein was significantly expressed in higher amounts among Y. pestis strains isolated from a recent plague outbreak. The possible use of electrophoretic profiles of outer membrane proteins of wild Y. pestis isolates as a tool for epidemiological studies and for the analysis of virulence determinants is discussed.

Type
Research Article
Information
Epidemiology & Infection , Volume 103 , Issue 3 , December 1989 , pp. 595 - 602
Copyright
Copyright © Cambridge University Press 1989

References

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