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Effect of stool storage at room temperature on salmonella isolation from faeces

Published online by Cambridge University Press:  19 October 2009

R. W. S. Harvey  and
T. H. Price
T. H. Price
Affiliation:
Regional Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff, CF4 4XW
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Summary

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Stools, known to have contained salmonellas were cultured in three enrichment media on arrival at the laboratory. The three media were laboratory prepared selenite F, laboratory prepared Muller-Kauffmann tetrathionate and commercially obtained Oxoid Muller-Kauffmann tetrathionate (CM343). Where faecal samples were sufficiently large, they were stored at room temperature and re-examined using the same three enrichment broths. Whether fresh or stored stools were investigated, the laboratory prepared media were significantly more efficient than the commercial medium. In a straight comparison of the two laboratory prepared media, no significant difference in efficiency was evident with fresh stool samples. With stored specimens, however, Muller-Kauffmann tetrathionate was significantly better than selenite F. This finding might be relevant in examining samples delayed in the post.

Type
Research Article
Information
Journal of Hygiene , Volume 91 , Issue 2 , October 1983 , pp. 267 - 271
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Anon (1970). Can bacteriologist agree? Lancet i, 664.Google Scholar
Anon (1975). Meat and meat products – detection of salmonellae(reference method). International Standard ISO 3565–1975 (E). Geneva: International Organization for Standardization.Google Scholar
Edel, W. & Kampelmacheu, E. H. (1969). Salmonella isolation in nine European laboratories using a standardized technique. Bulletin of the World Health Organization 41, 297–300.Google ScholarPubMed
Harvey, R. W. S. (1956). Choice of a selective medium for the routine isolation of members of the salmonella group. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 15, 118–124.Google ScholarPubMed
Harvey, R. W. S. & Price, T. H. (1975). Studies on the isolation of Salmonella dublin. Journal of Hygiene 74, 369–374.CrossRefGoogle ScholarPubMed
Harvey, R. W. S. & Price, T. H. (1977). Observations on pre-enrichment for isolating Saltnonellas from sewage polluted natural water using Muller-Kauffmann tetrathionate broth prepared with fresh and desiccated ox bile. Journal of Applied Bacteriology 43, 145–148.CrossRefGoogle ScholarPubMed
Harvey, R. W. S. & Price, T. H. (1980). Salmonella isolation with Rappaport's medium after pre-enrichment in buffered peptone water using a series of inoculum ratios. Journal of Hygiene 85, 125–128.CrossRefGoogle ScholarPubMed
Harvey, R. W. S. & Price, T. H. (1982 a). A comparison of the R25 modification of Rappaport's enrichment medium with strontium chloride B for salmonella isolation from sewage polluted natural water. Journal of Hygiene 89, 111–117.CrossRefGoogle Scholar
Harvey, R. W. S. & Price, T. H. (1982 b). Salmonella isolation and identification techniques alternative to the standard method. In Isolation and Identification Methods fur Food Poisoning Organisms, p. 51. Society for Applied Bacteriology Technical Series, no. 17 (ed. Corry, , Roberts, and Skinner, ). Academic Press: London.Google Scholar
Harvey, R. W. S., Price, T. H. & Crone, P. B. (1975). Quality control tests of two salmonella enrichment media using different inocula. Journal of Hygiene 74, 375–384.CrossRefGoogle ScholarPubMed
Harvey, R. W. S., Price, T. H. & Xirouchaki, E. (1979). Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique. Journal of Hygiene 83, 451–460.CrossRefGoogle ScholarPubMed
Harvey, R. W. S. & Thomson, S. (1953). Optimum temperature of incubation for isolation of salmonellae. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 12, 149–150.Google ScholarPubMed
Iveson, J. B. & Mackay-Scollay, E. M. (1969). Strontium chloride and strontium selenite enrichment media in the isolation of Salmonella. Journal of Hygiene 67, 457–464CrossRefGoogle ScholarPubMed
Kauffmann, F. (1930). Ein kombiniertes Anreicherungsverfahren fur Typhus-und Paratyphusbazillen. Zentralblaft fur Bakteriologie, Parasitenkunde und Infektionskrankheiten I. Abt. Orig. 119, 148–152.Google Scholar
Kauffmann, F. (1935). Weitere Erfahrungen mit kombinierten Anreicherungsverfahren für Salmonella bazillen. Zeitschrift für Hygiene und Infektionskrankheiten 117, 26–32.CrossRefGoogle Scholar
Leifson, E. (1936). New selenite enrichment media for the isolation of typhoid and paratyphoid (salmonella) bacilli. American Journal of Hygiene 24, 423–432.Google Scholar
McCov, J. H. (1975). Trends in salmonella food poisoning in England and Wales 1941–72. Journal of Hygiene 74, 271–282.CrossRefGoogle Scholar
Muller, L. (1923). Un nouveau milieu d'enrichissement pour la recherche du bacille typhique et des paratyphiques. Comptes liendus des Seances de la Societe de Biologie et des ses Filiales 89, 434–437.Google Scholar
Price, T. H. (1976). Isolation of Shigella sonnei by fluid media. Journal of Hygiene 77, 341–348.CrossRefGoogle ScholarPubMed
Read, R. B. & Reyes, A. L. (1968). Variation in plating efficiency of salmonellae on eight lots of brilliant green agar. Applied Microbiology 16, 740–748.CrossRefGoogle ScholarPubMed
Roberts, D., Boag, K., Hall, M. L. M. & Ship, C. R. (1975). The isolation of salmonellas from British pork sausage meat. Journal of Hygiene 75, 173–184.CrossRefGoogle Scholar
Stokes, E. J. (1978). Quality control in the bacteriological laboratory. Health Care World Wide. Intake Issue No. 58, 1. Abbott Laboratories Ltd.Google Scholar
Taylor, W. I. & Schelhart, D. (1968). Isolation of shigellae. V. Comparison of enrichment broths with stools. Addendum. Applied Microbiology 16, 1383–1386.CrossRefGoogle Scholar
Thomson, S. (1955). The numbers of pathogenic bacilli in faeces in intestinal diseases. Journal of Hygiene 52, 217–224.CrossRefGoogle Scholar
Van Schothorst, M., Van Leusden, M., Jeunink, J. & De Dreu, J. (1977). Studies on the multiplication of salmonellae in various enrichment media at different incubation temperatures. Journal of Applied Bacteriology 42, 157–163.CrossRefGoogle ScholarPubMed
Vassiliadis, P., Pateraki, E., Papadakis, J. & Trichopoulos, D. (1974). Evaluation of the growth of salmonellae in Rappaport's broth and in Muller-Kauffmann's tetrathionate broth. Journal of Applied Bacteriology 37, 411–418.CrossRefGoogle ScholarPubMed