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The effect of pre-enrichment on recovery of Streptococcus agalactiae, Staphylococcus aureus and mycoplasma from bovine milk

Published online by Cambridge University Press:  15 May 2009

M. C. Thurmond ,
J. W. Tyler ,
D. M. Luiz ,
C. A. Holmberg  and
J. P. Picanso
M. C. Thurmond
Affiliation:
Departments of Medicine, School of Veterinary Medicine/University of California (Davis), 18830 Road 112, Tulare, CA 93274, USA
J. W. Tyler
Affiliation:
Departments of Clinical Pathology, School of Veterinary Medicine/University of California (Davis), 18830 Road 112, Tulare, CA 93274, USA
D. M. Luiz
Affiliation:
and the Departments of Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine/University of California (Davis), 18830 Road 112, Tulare, CA 93274, USA
C. A. Holmberg
Affiliation:
Departments of Pathology, School of Veterinary Medicine/University of California (Davis), 18830 Road 112, Tulare, CA 93274, USA
J. P. Picanso
Affiliation:
and the Departments of Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine/University of California (Davis), 18830 Road 112, Tulare, CA 93274, USA
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The study was conducted to determine whether pre-enrichment would increase sensitivity of detecting Streptococcus (Str.) aqalactiae, Staphylococcus (S.) aureus, and mycoplasma in bovine milk. Two procedures were followed, one involving direct inoculation of milk on bovine blood agar, and the other involving preenrichment in broth followed by inoculation on agar. Logistic regression was used to predict the probability of isolation as a function of culture procedure and two additional covariates. the California Mastitis Test (CMT) score of the milk and the type of sample (indicating sample storage temperature and herd mastitis status). A total of 13778 milk samples was cultured for each of the three bacteria. By using results of both direct inoculation and pre-enrichment, the probability of isolation compared to use of direct inoculation only and adjusted for effects of other variables was increased 3·6-fold for Str. agalactiae, 1·6-fold for S. aureus and 1·7- fold for mycoplasma. The probability of isolation for all three bacteria increased as the CMT score increased. For Str. agalactiae, there was a statistical interaction predicting that enrichment improved the odds of isolation more from milk with high CMT scores than from milk with low scores. Results indicate that preenrichment can substantially increase the sensitivity of bacteriological screening of dairy cows for mastitis caused by Str. agalactiae, S. aureus, and mycoplasma.

Type
Research Article
Information
Epidemiology & Infection , Volume 103 , Issue 3 , December 1989 , pp. 465 - 474
Copyright
Copyright © Cambridge University Press 1989

References

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