Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-23T21:22:42.894Z Has data issue: false hasContentIssue false
  • English
  • Français

Dose-response relationships in a microneutralization test for foot-and-mouth disease viruses

Published online by Cambridge University Press:  15 May 2009

J. C. Booth ,
M. M. Rweyemamu  and
T. W. F. Pay
J. C. Booth
Affiliation:
The Wellcome Foot-and-Mouth Disease Vaccine Laboratory, Pirbright, Woking, Surrey, GU24 0NQ
M. M. Rweyemamu
Affiliation:
The Wellcome Foot-and-Mouth Disease Vaccine Laboratory, Pirbright, Woking, Surrey, GU24 0NQ
T. W. F. Pay
Affiliation:
The Wellcome Foot-and-Mouth Disease Vaccine Laboratory, Pirbright, Woking, Surrey, GU24 0NQ
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Two-dimensional quantal microneutralization tests on foot-and-mouth disease viruses, in which neutralizing antibody activity was titrated against a serial range of virus doses, demonstrated a variety of dose-response curves some of which were rectilinear, others clearly curvilinear. Moreover, in the case of the non-linear responses obtained with some antisera, the shape of the curve was such that antibody titres recorded with doses of virus ranging from 103–105 TCD 50 were closely similar. Studies were carried out on the effect of varying the conditions of the test on the shape of the dose-response curve: significant differences were obtained after treatment of the antiserum–virus mixtures with anti-species globulin, and when the test was assayed in cells of differing susceptibility to infection.

Type
Research Article
Information
Journal of Hygiene , Volume 80 , Issue 1 , February 1978 , pp. 31 - 42
Copyright
Copyright © Cambridge University Press 1978

References

REFERENCES

Ashe, W. K. & Notkins, A. L. (1966). Neutralization of an infectious herpes simplex virus-antibody complex by anti-γ-globulin. Proceedings of the National Academy of Sciences of the U.S.A. 56, 447–51.CrossRefGoogle ScholarPubMed
Axler, D. A. & Crowell, R. L. (1968). Effect of anticellular serum on the attachment of enteroviruses to HeLa cells. Journal of Virology 2, 813–21.CrossRefGoogle ScholarPubMed
Brown, F. & Cartwright, B. (1963). Purification of radioactive foot-and-mouth disease virus. Nature, London 199, 1168–70.CrossRefGoogle ScholarPubMed
Brown, F., Hyslop, N. St. G., Crick, J. & Morrow, A. W. (1963). The use of acetylethy-leneimine in the production of inactivated foot-and-mouth disease vaccines. Journal of Hygiene 61, 337–44.CrossRefGoogle ScholarPubMed
Cihak, J. (1973). Neutralization of infectious ME (Maus–Elberfeld) virus antibody complexes by anti-γ-globulin antibody. Medical Microbiology and Immunology 159, 73–82.CrossRefGoogle ScholarPubMed
De Castro, M. P. (1964). Behaviour of the foot-and-mouth disease virus in cell cultures; susceptibility of the IB-RS-2 cell line. Archivos do Instituto biologico, São Paulo 31, 63–78.Google Scholar
Fazekas de St Groth, S. (1961). Evaluation of quantal neutralization tests. Nature, London 181, 891–3.CrossRefGoogle Scholar
Fazekas de St Groth, S. & Webster, R. G. (1963). The neutralization of animal viruses. III. Equilibrium conditions in the influenza virus-antibody system. Journal of Immunology 90, 140–50.Google Scholar
Fazekas de St Groth, S., Withell, J. & Lafferty, K. J. (1958). An improved assay method for neutralizing antibodies against influenza viruses. Journal of Hygiene 56, 415–26.CrossRefGoogle Scholar
Gard, S. (1957). Immunoinactivation of poliovirus. Archiv für die gesamte Virusforschung 7, 449–60.CrossRefGoogle ScholarPubMed
Horsfall, F. L. (1939). Neutralization of epidemic influenza virus. The linear relationship between the quantity of serum and the quantity of virus neutralized. Journal of Experimental Medicine 70, 202–22.Google ScholarPubMed
Horsfall, F. L. & Lennette, E. H. (1941). Neutralization of influenza A virus by human serum. Journal of Experimental Medicine 73, 327–33.CrossRefGoogle ScholarPubMed
Huggett, D. O., Rodriguez, J. E. & McKee, A. P. (1972). Infectious antibody-reovirus complexes. Infection and Immunity 6, 996–1002.CrossRefGoogle ScholarPubMed
Karber, G. (1931). Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Archiv für experimentelle Pathologie und Pharmakologie 162, 480–3.CrossRefGoogle Scholar
Kjellén, L. E. & Schlesinger, R. W. (1959). Influence of host cell on residual infectivity of neutralized vesicular stomatitis virus. Virology 7, 236–9.CrossRefGoogle ScholarPubMed
Martin, W. B. & Chapman, W. G. (1961). The tissue culture colour test for assaying the virus and neutralizing antibody of foot-and-mouth disease virus and its application to the measurement of immunity in cattle. Research in Veterinary Science 2, 53–61.CrossRefGoogle Scholar
Rweyemamu, M. M., Booth, J. C. & Pay, T. W. F. (1977). Neutralization kinetics studies with type SAT2 foot-and-mouth disease virus strains. 1. Factors that influence the rate and pattern of neutralization. Journal of Hygiene 78, 99–110.CrossRefGoogle ScholarPubMed
Stalder, H., Oxman, M. N. & Herrman, K. L. (1975). Herpes simplex virus microneutralization: a simplification of the test. Journal of Infectious Diseases 131, 423–30.CrossRefGoogle ScholarPubMed
Timbury, Morag C. (1963). Antigenic variation in amnion cells after growth in tissue culture in relation to the inhibition of enteroviruses by anticellular serum. Virology 19, 501–8.CrossRefGoogle Scholar
Tyrrell, D. A. J. & Horsfall, F. L. (1953). Neutralization of viruses by homologous immune serum. 1. Quantitative studies on factors which affect the neutralization reaction with Newcastle disease, influenza A and bacterial virus, T3. Journal of Experimental Medicine 97, 845–61.CrossRefGoogle Scholar
Wallis, C. & Melnick, J. L. (1967). Virus aggregation as the cause of the non-neutralizable persistent fraction. Journal of Virology 1, 478–88.CrossRefGoogle ScholarPubMed
Wild, T. F. & Brown, F. (1967). Nature of the inactivating action of trypsin on foot-and-mouth disease virus. Journal of General Virology 1, 247–50.CrossRefGoogle ScholarPubMed