Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-08T04:20:01.122Z Has data issue: false hasContentIssue false
  • English
  • Français

Do intestinal parasites interfere with the seroepidemiologic surveillance of Schistosoma mansoni infection?

Published online by Cambridge University Press:  15 May 2009

B. Alarcón De Noya ,
C. Colmenares ,
S. Losada ,
Z. Fermin ,
G. Masroua ,
L. Ruiz ,
L. Soto  and
O. Noya
B. Alarcón De Noya*
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
C. Colmenares
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
S. Losada
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
Z. Fermin
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
G. Masroua
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
L. Ruiz
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
L. Soto
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
O. Noya
Affiliation:
Sección de Biohelmintiasis, Instituto de Medicina Tropical, Universidad Central de Venezuela
*
* Address for correspondence: Dra. Belkisyolé Alarcón de Noya, Laboratorio de Estudios para Malaria. Universidad Central de Venezuela. Apartado Postal 4417. Caracas, 1010-A, Venezuela.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In view of the known cross-reactivity of sera from patients with intestinal parasites to some Schistosoma mansoni antigens, field work was conducted in an area of Venezuela non-endemic for schistosomiasis using the routine immunoenzymatic assay (ELISA) with soluble egg antigen (SEA). False positive reactions represented 15·3% of the total population as determined by SEA–ELISA. SEA-immunoblotting of the false positive sera indicated that protein fractions of 91 and 80 kDa appear to be responsible for cross-reactivity. Sera from hookworm infected individuals produced a higher frequency and intensity of cross-reaction than other sera. SEA-fractions of 105, 54, 46, 42, 32, 25 and 15 kDa were the most specific.

Type
Special Article
Information
Epidemiology & Infection , Volume 116 , Issue 3 , June 1996 , pp. 323 - 329
Copyright
Copyright © Cambridge University Press 1996

References

REFERENCES

1.Alarcón de Noya, B, Noya, O, Balzan, C, Cesari, I. New approaches for the control and eradication of schistosomiasis in Venezuela. Mem do Inst Oswaldo Cruz 1992; 87: 227–31.Google Scholar
2.Mott, KE, Dixon, H. Collaborative study on antigens for a immunodiagnosis of schistosomiasis. Bull WHO 1982; 60: 729–53.Google Scholar
3.Hillyer, GV, Ruiz, Tiben E, Knight, WB, Gomez de Rios, I, Peley, RP. Immunodiagnosis of infection with Schistosoma mansoni: comparison of ELISA, radio-immunoassay and precipitation tests performed with antigens from eggs. Am J Trop Med Hyg 1979; 28: 661–9.Google Scholar
4.Spencer, L, Alarcón de Noya, B, Noya, O, Masroua, G. Prueba de precipitación circumoval y ELISA conantígenos solubles en el diagnóstico de la esquistosomiasis. GEN 1991; 45: 77–83.Google Scholar
5.Alarcón de Noya, B, Spencer, L, Noya, O. Pre and post-treatment immunodiagnostic evaluation in human schistosomiasis mansoni. Mem do Inst Oswaldo Cruz 1992; 87: 271–6.CrossRefGoogle Scholar
6.Correa-Oliveira, R, Oliveira, GC, Golgher, DB et al. , Analysis of antibody responses of Schistosoma mansoni infected patients against schistosomal antigens. Mem do Inst Oswaldo Cruz 1987; 82: 179–81.Google Scholar
7.Correa-Oliveira, R, Dusse, LMS, Viana, IRC, Colley, DG, Carvalho, OS, Gazzinelli, G. Human antibody responses against schistosomal antigens. Antibodies from patients with Ancylostoma, Ascaris lumbricoides or Schistosoma mansoni infections react with schistosome antigens. Am J Trop Med Hyg 1988; 38: 348–55.CrossRefGoogle ScholarPubMed
8.Pessõa, SB, Martins, AV. Pessõa parasitologí médica. Guanabara, Koogan SA, eds. Rio de Janeiro 1982: 832.Google Scholar
9.Boros, DL, Warren, KS. Delayed hypersensitivity-type granuloma formation and dermal reaction induced and elicited by a soluble factor isolated from Schistosoma mansoni eggs. J Exp Med 1970; 132: 488–507.Google Scholar
10.Lowry, OH, Rossenbrough, NJ, Farr, AL, Randall, RJ. Protein measurement with the Folin reagent. J Biol Chem 1951; 193: 265–75.Google Scholar
11.Voller, A, Bartlett, A, Bidwell, D. Enzyme immunoassays for parasitic diseases. Trans Roy Soc Trop Med Hyg 1976; 70: 98–105.Google Scholar
12.Oliver-González, J. Anti-egg precipitin in sera of humans infected with Schistosoma mansoni. J Infect Dis 1954; 95: 86–91.Google Scholar
13.Laemli, UK. Cleavage of structural proteins during the assembly of the head of bacteriophague T4. Nature 1970; 227: 680–5.Google Scholar
14.Towbin, H, Staehelin, T, Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Nat Acad Sciences 1979; 76: 4350–4.Google Scholar
15.Pelley, RP, Hillyer, GV. Demonstration of a common antigen between Schistosoma mansoni and Fasciola hepatica. Am J Trop Med Hyg 1978; 27: 1192–4.Google Scholar
16.Hillyer, GV, Serrano, AE. The antigens of Paragonimus westermani, Schistosoma mansoni and Fasciola hepatica adult worms. Am J Trop Med Hyg 1983; 32: 350–8.Google Scholar
17.Hanna, REB, Hillyer, GV. Fasciola hepatica and Schistosoma mansoni: Immunofluorescent antigen localization and cross-reactivity. Exp Parasitol 1984; 57: 1–14.Google Scholar
18.Aronstein, WS, Daton, JP, Strand, M. A Schistosoma mansoni surface glycoprotein cross-reactive with a T1 antigen of Fasciola hepatica. Am J Trop Med Hyg 1985; 34: 889–97.Google Scholar
19.Aronstein, WS, Dalton, JP, Weiss, JB, Strand, M. Identification and characterization of a major Schistosoma mansoni glycoprotein antigen crossreactive with Fasciola hepatica. Am J Trop Med Hyg 1985; 34: 879–88.Google Scholar
20.Aronstein, WS, Lewis, SA, Norden, AP, Dalton, JP, Strand, M. Molecular identity of a major antigen of Schistosoma mansoni which cross-reacts with Trichinella spiralis and Fasciola hepatica. Parasitol 1986; 92: 133–51.Google Scholar
21.Linder, E, Thors, C, Lundin, L et al. , Schistosome antigen GP50 is responsible for serological cross-reactivity with Trichinella spiralis. J Parasitol 1992; 78: 999–1005.CrossRefGoogle ScholarPubMed
22.Botero, D. Persistencia de parasitosis intestinales endémicas en América Latina. Bol Ofic Sanit Panam 1981; 90: 39–46.Google Scholar
23.Morales, G, Pino, LA, Rodriguez, E. Estudio de las geohelmintiasis en humanos en las zonas marginales de la ciudad de Trujillo, Venezuela. Bol Direc Malariol San Amb 1984; XXIV: 71–8.Google Scholar
24.Doenhoff, MJ, Butterworth, AE, Hayes, RJ et al. , Seroepidemiology and serodiagnosis of schistosomiasis in Kenya using crude and purified egg antigens of Schistosoma mansoni in ELISA. Trans Roy Soc Trop Med Hyg 1993; 87: 42–8.Google Scholar
25.Hillyer, GV, Gomez de Rios, I. The enzyme-linked immunosorbent assay (ELISA) for the immuno-diagnosis of schistosomiasis. Am J Trop Med Hyg 1979; 28: 237–41.CrossRefGoogle Scholar
26.Mc Laren, M, Long, EG, Goodgame, RW, Lillywhite, JE. Application of the enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of Schistosoma mansoni infections in St. Lucia. Trans Roy Soc Trop Med Hyg 1979; 73: 636–9.CrossRefGoogle ScholarPubMed
27.Alarcón de Noya, B, Pujol, FH. Diagnóstico inmunológico de la esquistosomiasis mansoni. Interciencia 1990; 15: 95–101.Google Scholar
28.Ramos, A, Cesari, I. Evaluatión de varias preparaciones solubles de huevos de Schistosoma mansoni, como antígenos para serodiagnóstico de la esquistosomiasis. ActaCient Ven 1991; 42: 202.Google Scholar
29.Noya, O, Losada, S, Alarcón de Noya, B et al. , Effect of chemotherapy on immune response to egg antigens of Schistosoma mansoni in chronically infected children from areas of low transmission. Parasite Immunol 1995; 17: 111–7.Google Scholar