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Differentiation of the serological response to Yersinia enterocolitica and Brucella abortus in cattle

Published online by Cambridge University Press:  15 May 2009

M. J. Corbel  and
G. A. Cullen
M. J. Corbel
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge
G. A. Cullen
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge
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The serological responses of cattle to inoculation with Brucella abortus and Yersinia enterocolitica type IX were compared. Complete cross-reactions were found in serum agglutination, antiglobulin, complement fixation and Rose Bengal plate tests. The cross-reaction between Br. abortus and Y. enterocolitica IX was confirmed by immunodiffusion tests. Although antibodies specific for each organism could also be detected by immunodiffusion tests with high titre rabbit or bovine sera, these tests were insufficiently sensitive for routine diagnostic use.

A quantitative Rose Bengal plate test, using Rose Bengal stained Br. abortus and Y. enterocolitica IX, was developed which enabled the antibody responses to the two organisms to be differentiated. The specificity of this test was confirmed by cross-absorption experiments and its sensitivity was sufficient to permit evaluation of all bovine sera giving positive reactions to the serum agglutination test.

Type
Research Article
Information
Journal of Hygiene , Volume 68 , Issue 4 , December 1970 , pp. 519 - 530
Copyright
Copyright © Cambridge University Press 1970

References

REFERENCES

Ahvonen, P., Jansson, E. & Aho, K. (1969). Marked cross-agglutination between Brucellae and a subtype of Yersinia enterocolitica. Acta pathologica et microbiologica scandinavica 75, 291.Google Scholar
Ahvonen, P. & Sievers, K. (1969). Yersinia enterocolitica infection associated with Brucella agglutinins. Acta medica scandinavica 185, 121.CrossRefGoogle ScholarPubMed
Alessandrini, A. & Sabatucci, M. (1931). A proposito della reazione di agglutinazione aspecifica alla tripaflavina. Annali d'igiene sperimentale 41, 852.Google Scholar
Becht, H. (1962). Untersuchungen über die Pseudotuberkulose beim Chinchilla. Deutsche tierärzlische Wochenschrift 69, 626.Google Scholar
Boyce, K. J. & Edgar, A. W. (1966). Production of freeze-dried Brucella abortus strain 19 vaccine using cells produced by continuous culture. Journal of Applied Bacteriology 29, 401.CrossRefGoogle Scholar
Brinley-Morgan, W. J. (1967). The serology of bovine brucellosis. Veterinary Record 80, 612.Google Scholar
Brinley-Morgan, W. J., MacKinnon, D. J., Lawson, J. & Cullen, G. A. (1969). The Rose Bengal plate agglutination test in the diagnosis of brucellosis. Veterinary Record 85, 636.CrossRefGoogle Scholar
Cherry, W. B., Goldman, M. & Carski, T. R. (1960). Fluorescent antibody techniques in the diagnosis of communicable diseases. U.S. Department of Health, Education and Welfare, Atlanta.Google Scholar
Coombs, R. R. A., Mourant, A. E. & Race, R. R. (1945). Detection of weak and ‘incomplete’ Rh agglutinins: a new test. Lancet ii, 15.Google Scholar
Daniels, J. J. H. M. (1963). Untersuchungen an als Pasteurella pseudotuberculosis diagnostizierten Stammen von Chinchillas. Zentralblatt für Veterinärmedizin 10, 413.Google Scholar
Dickinson, A. & Mocquot, G. (1961). Studies on the bacterial flora of the alimentary tract of pigs. 1. Enterobacteriaceae and other Gram-negative bacteria. Journal of Applied Bacteriology 24, 252.Google Scholar
Frederiksen, W. (1964). A study of some Yersinia pseudotuberculosis-like bacteria (‘Bacterium enterocoliticum and “Pasteurella X” ’). Proceedings of the XIV Scandinavian Congress of Pathology and Microbiology. Oslo.Google Scholar
Goyon, M. (1969). Endocardite végétante à Yersinia enterocolitica chez un bovin. Recueil de médicine vétérinaire de L'École d' Alfort 45, 61.Google Scholar
Huhtala, E. (1963). L'éradication de la Brucellose bovine en Finlande. Bulletin. Office international des épizooties 60, 447.Google Scholar
LoGrippo, G. A. & Hartman, F. W. (1955). Antigenicity of β-propiolactone-inactivated virus vaccines. Journal of Immunology 75, 123.CrossRefGoogle ScholarPubMed
Mair, N. S., Schubert, F. N. & Harbourne, J. F. (1970). Yersinia enterocolitica infection in the bush-baby (Galago). Veterinary Record 86, 69.Google Scholar
Mallman, W. L. (1930). The interagglutinability of members of the Pasteurella and Brucella genera. Journal of the American Veterinary Medical Association 77, 636.Google Scholar
Miles, A. A. & Misra, S. S. (1938). The estimation of the bactericidal power of the blood. Journal of Hygiene 38, 732.Google ScholarPubMed
Mollaret, H. H. (1968). Revue. Acquisitions nouvelles dans le domaine des Yersinioses. Bulletin de l' Institut Pasteur, Paris, 66, 871.Google Scholar
Mollaret, H. H. & Chevalier, A. (1964). Contribution á l'étude d'un nouveau groupe de germes proches du bacille de Malasse et Vignal. 1. Caractères cultureux et biochimiques. Annales de l' Institut Pasteur, Paris 107, 121.Google Scholar
Mollaret, H. H. & Guillon, J. C. (1965). Contribution á l'étude d'un nouveau groupe de germes (Yersinia enterocolitica) proches du bacille de Malassez et Vignal. II. Pouvoir pathogène expérimental. Annales de l' Institut Pasteur, Paris, 109, 608.Google Scholar
Mollaret, H. H. & Lucas, A. (1965). Sur les particularités biochimiques des souches de Yersinia enterocolitica isolées chez les lièvres. Annales de l' Institut Pasteur, Paris 108, 121.Google Scholar
Morse, E., Ristic, M., Robertstad, G. & Schneider, D. (1953). Cross-agglutination reactions among Brucella, Vibrio and other micro-organisms. American Journal of Veterinary Research 14, 324.Google Scholar
Niléhn, B. (1967).Studies on Yersinia enterocolitica. Characterization of 28 strains from human and animal sources. Acta pathologica et microbiologica scandinavica 69, 83.CrossRefGoogle Scholar
Ouchterlony, O. (1953). Antigen-antibody reactions in gels. Acta pathologica et microbiologica scandinavica 32, 231.Google Scholar
Schleifstein, J. & Coleman, M. (1943). Bacterium enterocoliticum. Annual Report of the Division of Laboratories and Research, New York State Department of Health, no. 56.Google Scholar
U.S. Department or Agriculture, National Animal Diseases Laboratory, Diagnostic Reagents Division, Ames, Iowa (1965). Diagnostic reagents manual 65C.Google Scholar
Winblad, S., Niléhn, B. & Sternby, N. H. (1966). Yersinia enterocolitica (Pasteurella X) in human enteric infections. British Medical Journal ii, 1363.Google Scholar
WHO, Monograph, no. 19 (1953). Advances in the control of zoonoses.Google Scholar