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Differentiation of Achromobacter-like strains from human blood by DNA restriction endonuclease digest and ribosomal RNA gene probe patterns

Published online by Cambridge University Press:  15 May 2009

B. Holmes ,
M. Costas ,
A. C. Wood ,
R. J. Owen  and
D. D. Morgan
B. Holmes
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, England
M. Costas
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, England
A. C. Wood
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, England
R. J. Owen
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, England
D. D. Morgan
Affiliation:
National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, England
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Variation amongst Achromobacter-like strains was examined by DNA restriction endonuclease digestion and rDNA gene patterns generated using a non-radioactive probe. Chromosomal DNA was extracted from 12 cultures representing Achromobacter groups B, E and F, all from human blood cultures. DNA fingerprinting using EcoRI, HaeIII or HindIII sub-divided the strains in a similar manner to that obtained by their protein patterns. The HaeIII patterns, with their small number of bands, were the easiest to interpret. The EcoRI patterns included a species-specific triplet of bands but minor band patterns allowed further differentiation. The Achromobacter group F strains comprised a separate taxon and were distinct from the group B and E strains by all techniques examined. The study demonstrates that, in addition to total DNA digest analysis, rDNA gene restriction patterns provide a simple but discriminatory electrophoretic method for distinguishing within Achromobacter groups B and E.

Type
Research Article
Information
Epidemiology & Infection , Volume 105 , Issue 3 , December 1990 , pp. 541 - 551
Copyright
Copyright © Cambridge University Press 1990

References

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